Functional roles of neurotrophic factors in the olfactory nervous system

神经营养因子在嗅觉神经系统中的功能作用

• 批准号: 08457003
• 负责人: MORIIZUMI Tetsuji
• 金额: $ 0.77万
• 依托单位: Shinshu Univ., Faculty of Med.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457003/
• 关键词: Olfaction; Olfactory bulb; Horizontal limb of the diagonal band of Broca; Neurotrophic factors; Nerve growth factor; Cholinergic neurons; 神経細胞の生存・維持; 神経細胞死; 嗅上皮; 受容体

We investigated survival of cholinergic neurons in the horizontal limb of the diagonal band of Broca (HLDB) following lesions of olfactory-related areas expressing neurotrophic factors in the rats. In order to label HLDB neurons projecting to the olfactory bulb, a retrograde fluorescent neuronal tracer (fast blue, FB) was injected into the bulb. After FB injections, we made lesions in the anterior olfactory nucleus (AON), and combined lesions in the AON and the olfactory bulb (OB). After 4 weeks'survival, the rats were perfused with a fixative, and the brains were removed. Frozen brain sections were serially made, and FB-positive HLDB neurons were counted to know neuronal survival in the control and lesioned rats. The brain sections were, in part, processed for immunohistochemistry for choline acetyltransferase (ChAT) to know survival of cholinergic HLDB neurons. In the AON-lesioned rats, no neuronal cell death occurred in the HLDB.In the AON・OB-lesioned rats, neuronal cell death occurred in the HLDB with marked decrease of the number of FB-positive neurons (n=124) compared with that of FB-positive neurons of the control rats (n=547). ChAT-positive HLDB neurons were also decreased in number in the AON・OB-lesioned rats. Thus these results show that cholinergic HLDB neurons are dependent, in their survival, on neurotrophic factors produced in the AON and OB.

在大鼠嗅觉相关区损伤表达神经营养因子后，我们研究了Broca斜角带水平支(HLDB)胆碱能神经元的存活情况。为了标记投射到嗅球的HLDB神经元，将一种逆行荧光示踪剂(快速蓝，FB)注入嗅球。FB注射后，我们在前嗅核(AON)进行损伤，并在AON和嗅球(OB)进行联合损伤。存活4周后，灌流固定剂，取出脑组织。连续制作冰冻切片，计数FB阳性HLDB神经元，以了解对照组和损毁大鼠神经元的存活情况。部分脑切片用于胆碱乙酰转移酶(ChAT)的免疫组织化学处理，以了解胆碱能HLDB神经元的存活情况。AON损毁大鼠HLDB区未见神经细胞死亡，AON·OB损毁大鼠HLDB区神经细胞死亡，FB阳性神经元数(n=124)较对照组(n=547)明显减少。Aon·OB损毁组大鼠HLDB区ChAT阳性神经元数量也明显减少。因此，这些结果表明，胆碱能HLDB神经元的存活依赖于AON和OB产生的神经营养因子。

项目成果

Miwa, T.et al.: "Retrograde transport of nerve growth factor from olfactory bulb to olfactory epithelium" NeuroReport. (in press). (1988)

Miwa, T.等人：“神经生长因子从嗅球到嗅上皮的逆行转运”NeuroReport。

Miwa,T.et al.: "Retrograde transport of nerve growth factor from olfactory bulb to olfactory epithelium" Neuro Report. (in press). (1998)

Miwa,T.等人：“神经生长因子从嗅球到嗅上皮的逆行转运”神经报告。

Miwa, T.et al.: "Retrograde transport of nerve growht factor from olfactory bulb to olfactory epithelium." NeuroReport. (in press). (1998)

Miwa, T.等人：“神经生长因子从嗅球逆行运输到嗅上皮。”