Establishment of a screening method to detect factors affecting neural cell migration

我们通过大鼠前脑吻侧迁移流（rostral migration stream， RMS）研究了神经细胞的迁移，得到了以下新发现。(A)在迁移过程中，RMS内的一小群细胞正在发生神经发生。RMS的calretinin免疫反应神经元共表达巢蛋白（20.8%）和多唾液化的神经细胞粘附分子（61.8%）。RMS中calretinin免疫反应神经元仍然表达未成熟的神经和神经元表型。(B) BrdU示踪法发现，嗅球内新形成的肾小球周围细胞在生命过程中大部分被替换。(C)采用^ 3h -胸腺嘧啶放射自显影和神经元制造细胞免疫组化相结合的方法，65.3-76.9%的球神经元在通过前脑迁移途径（RMS）迁移后，在6周内发生神经元细胞死亡。(D)通过阻止神经细胞迁移，我们证明了嗅球中局部神经干细胞的神经发生。此外，我们发现局部和迁移的神经干细胞在大脑中有不同的神经发生和胶质瘤发生。

We investigated neural cell migration through the rostral migratory stream (RMS) in the rat forebrain and obtained the new findings as follows.(A) Neurogenesis is ongoing in a small population of cells within the RMS during migration. Calretinin-immunoreactive neurons of the RMS co-express nestin (20.8%) and polysialylated neural cell adhesion molecule (61.8%). Calretinin-immunoreactive neurons in the RMS still express immature neural and neuronal phenotypes.(B) By the BrdU tracing method, newly formed periglomerular cells in the olfactory bulb are mostly replaced during life.(C) With the combined method of autoradiography using ^3H-thymidine and immunohistochemistry for a neuronal maker, 65.3-76.9% of bulbar neurons, following migration through the forebrain migratory route (RMS), undergo neuronal cell death during six weeks.(D) By preventing neural cell migration, we demonstrated neurogenesis by local neural stem cells in the olfactory bulb. Further, we revealed differential neurogenesis and gliogenesis by local and migrating neural stem cells in the brain.