Establishment of a screening method to detect factors affecting neural cell migration

神经细胞迁移影响因素筛选方法的建立

• 批准号: 11670007
• 负责人: MORIIZUMI Tetsuji
• 金额: $ 1.28万
• 依托单位: Shinshu Univ., Faculty of Med.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670007/
• 关键词: Cell migration; Neural stem cells; Neuronal differentitation; Olfactory bulb; Calretinin; 神経系乾細胞; ニューロン形成; 神経細胞; 細胞分化; ポリシアル酸; ネスチン

We investigated neural cell migration through the rostral migratory stream (RMS) in the rat forebrain and obtained the new findings as follows.(A) Neurogenesis is ongoing in a small population of cells within the RMS during migration. Calretinin-immunoreactive neurons of the RMS co-express nestin (20.8%) and polysialylated neural cell adhesion molecule (61.8%). Calretinin-immunoreactive neurons in the RMS still express immature neural and neuronal phenotypes.(B) By the BrdU tracing method, newly formed periglomerular cells in the olfactory bulb are mostly replaced during life.(C) With the combined method of autoradiography using ^3H-thymidine and immunohistochemistry for a neuronal maker, 65.3-76.9% of bulbar neurons, following migration through the forebrain migratory route (RMS), undergo neuronal cell death during six weeks.(D) By preventing neural cell migration, we demonstrated neurogenesis by local neural stem cells in the olfactory bulb. Further, we revealed differential neurogenesis and gliogenesis by local and migrating neural stem cells in the brain.

我们研究了大鼠前脑吻侧迁移流（RMS）中神经细胞的迁移，获得了如下新发现。(A)在迁移过程中，RMS内的一小部分细胞正在进行神经发生。RMS的钙视黄蛋白免疫反应阳性神经元共表达巢蛋白（20.8%）和多聚唾液酸化神经细胞粘附分子（61.8%）。RMS中的钙视黄蛋白免疫反应神经元仍然表达不成熟的神经和神经元表型。(B)通过BrdU示踪方法，嗅球中新形成的球周细胞在生命过程中大部分被替换。(C)结合使用^3H-胸苷的放射自显影和免疫组织化学的神经元标记方法，65.3-76.9%的延髓神经元在通过前脑迁移途径（RMS）迁移后，在6周内经历神经元细胞死亡。(D)通过阻止神经细胞迁移，我们证明了嗅球中局部神经干细胞的神经发生。此外，我们揭示了本地和迁移的神经干细胞在大脑中的差异神经发生和胶质细胞。