Analysis of virulence factors in Salmonella speces
Analysis of virulence factors in Salmonella speces
批准号:
10041189
负责人:
KURAZONO Hisao
金额:
$1.86万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C).
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999
中文摘要
根据已报道的沙门氏菌内毒素基因序列,设计特异性寡核苷酸引物,用于聚合酶链反应(PCR)检测临床标本和食品中分离的沙门氏菌。作为对照,使用不同的细菌菌株,包括肠道细菌。所有沙门氏菌菌株(567株,58个血清型)均显示预期PCR阳性结果。用胰酪胨大豆肉汤或沙门氏菌增菌肉汤增菌,PCR体系的检出限分别为每克粪便和碎肉样品1个细菌细胞。该PCR体系在公共卫生领域的实际应用具有一定的价值。
英文摘要
The specific oligonucleotide primers for Salmonella enerotoxin gene (stn 101 and stn 111) were designed according to the reported sequences for polymerase chain reaction (PCR) method to examine various strains of Salmonella isolated from clinical specimens and food samples. As the control, different bacterial strains including enteric bacteria were used. All of the Salmonella strains (567 strains with 58 serovar) showed positive results for the expected PCR. On the other hand, the other bacterial genera (224 strains of 30 different species) did not yield and PCR product for these specific primers.The detection limit of the PCR system was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Trypticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public health.
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P. Tapchaisri et al.: "Detection of Salmonella Contamination in Food Samples by Dot-ELISA, DNA Amplification and Bacterial Culture"Asian Pacific Journal of Allergy and Immunology. 17. 41-51 (1999)
P. Tapchaisri 等人:“通过点 ELISA、DNA 扩增和细菌培养检测食品样品中的沙门氏菌污染”《亚太过敏与免疫学杂志》。
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P.Tapchaisri et al: "Detection of Salmonalla antigen in clinical samples using dot-beot ELISA,DNA amplification and culture method" Mahidol Journal. (in press). (1999)
P.Tapchaisri 等人:“使用 dot-beot ELISA、DNA 扩增和培养方法检测临床样本中的沙门氏菌抗原”Mahidol Journal。
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Makino S, H. Kurazono, M. Chongsa-nguan, H. Hayashi, H. Cheun, S. Suzuki and T. Shirahata: "Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples"The Journal of Veterinary Medical Scie
Makino S、H. Kurazono、M. Chongsa-nguan、H. Hayashi、H. Cheun、S. Suzuki 和 T. Shirahata:“沙门氏菌特异性 PCR 系统的建立及其在食品和粪便检查中的应用
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作者:
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通讯作者:
P. Tapchaisri: "Detection of Salmonella Contamination in Food Samples by Dot-ELISA, DNA Amplification and Bacterial Culture"Asian Pacific Journal of Allergy and Immunology. 17. 41-51 (1999)
P. Tapchaisri:“通过斑点 ELISA、DNA 扩增和细菌培养检测食品样品中的沙门氏菌污染”《亚太过敏与免疫学杂志》。
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通讯作者:
S. Makino et al.: "Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples"The Journal of Veterinary Medical Science. 61・11. 1245-1247 (1999)
S. Makino 等人:“沙门氏菌特异性 PCR 系统的建立及其在食品和粪便样本检查中的应用”《兽医医学杂志》61・11(1999 年)。
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共 7 条
Basic research on the safety of mucosal vaccine candidate substances against urinary pathogenic Escherichia coli infection
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批准号:18K07098
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2018
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负责人:KURAZONO Hisao
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依托单位:
Analysis of Uropathogenic-specific protein and development of the mucosal vaccine by using of USP
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批准号:20590436
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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财政年份:2008
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负责人:KURAZONO Hisao
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依托单位:
Analysis of Uropathogenic-specific protein and development of the mucosal vaccine by using of USP
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批准号:18590433
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.57万
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财政年份:2006
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负责人:KURAZONO Hisao
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依托单位:
Surveillance of Bacterial Intestinal diseases in Southeast Asian countries
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批准号:16406013
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.23万
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财政年份:2004
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负责人:KURAZONO Hisao
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依托单位:
Analysis of bacterial infectious-pathogens in Japan and Asian countries.
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批准号:13576013
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.45万
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财政年份:2001
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负责人:KURAZONO Hisao
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依托单位:
Analysis of virulence factors in Uropathogenic Escherichia coli
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批准号:10670249
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.05万
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财政年份:1998
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负责人:KURAZONO Hisao
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依托单位:
Analysis of virulence factors in Salmonella species
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批准号:09041172
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$0.96万
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财政年份:1997
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负责人:KURAZONO Hisao
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依托单位:
海外基金