Analysis of virulence factors in Salmonella speces

沙门氏菌属毒力因子分析

• 批准号: 10041189
• 负责人: KURAZONO Hisao
• 金额: $ 1.86万
• 依托单位: Okayama University (1999)University of Tsukuba (1998)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10041189/
• 关键词: salmonella; enterotoxin; PCR; fecal; meat; public health; サルモネラ; 食中毒; DNAプローブ; 検出法; ELISA; 蛋白毒素; 抗体

The specific oligonucleotide primers for Salmonella enerotoxin gene (stn 101 and stn 111) were designed according to the reported sequences for polymerase chain reaction (PCR) method to examine various strains of Salmonella isolated from clinical specimens and food samples. As the control, different bacterial strains including enteric bacteria were used. All of the Salmonella strains (567 strains with 58 serovar) showed positive results for the expected PCR. On the other hand, the other bacterial genera (224 strains of 30 different species) did not yield and PCR product for these specific primers.The detection limit of the PCR system was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Trypticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public health.

根据已报道的序列设计特异性的沙门氏菌肠毒素基因寡核苷酸引物（stn 101和stn 111），用于临床和食品分离沙门氏菌的聚合酶链反应（PCR）检测。采用不同菌株作为对照，包括肠道细菌。所有沙门氏菌（567株，58个血清型）的PCR结果均为阳性。另一方面，其他细菌属（30个不同种的224株）对这些特异性引物没有产生和PCR产物。PCR系统检测限为每克粪便和肉糜样品1个细菌细胞，分别采用胰酶豆汤或沙门氏菌富集肉汤进行富集。结果表明，该PCR系统在公共卫生领域具有实际应用价值。

项目成果

P. Tapchaisri et al.: "Detection of Salmonella Contamination in Food Samples by Dot-ELISA, DNA Amplification and Bacterial Culture"Asian Pacific Journal of Allergy and Immunology. 17. 41-51 (1999)

P. Tapchaisri 等人：“通过点 ELISA、DNA 扩增和细菌培养检测食品样品中的沙门氏菌污染”《亚太过敏与免疫学杂志》。

P.Tapchaisri et al: "Detection of Salmonalla antigen in clinical samples using dot-beot ELISA,DNA amplification and culture method" Mahidol Journal. (in press). (1999)

P.Tapchaisri 等人：“使用 dot-beot ELISA、DNA 扩增和培养方法检测临床样本中的沙门氏菌抗原”Mahidol Journal。

P. Tapchaisri: "Detection of Salmonella Contamination in Food Samples by Dot-ELISA, DNA Amplification and Bacterial Culture"Asian Pacific Journal of Allergy and Immunology. 17. 41-51 (1999)

P. Tapchaisri：“通过斑点 ELISA、DNA 扩增和细菌培养检测食品样品中的沙门氏菌污染”《亚太过敏与免疫学杂志》。

Makino S, H. Kurazono, M. Chongsa-nguan, H. Hayashi, H. Cheun, S. Suzuki and T. Shirahata: "Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples"The Journal of Veterinary Medical Scie

Makino S、H. Kurazono、M. Chongsa-nguan、H. Hayashi、H. Cheun、S. Suzuki 和 T. Shirahata：“沙门氏菌特异性 PCR 系统的建立及其在食品和粪便检查中的应用

S. Makino et al.: "Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples"The Journal of Veterinary Medical Science. 61・11. 1245-1247 (1999)

S. Makino 等人：“沙门氏菌特异性 PCR 系统的建立及其在食品和粪便样本检查中的应用”《兽医医学杂志》61・11（1999 年）。