Surveillance of Bacterial Intestinal diseases in Southeast Asian countries

东南亚国家细菌性肠道疾病监测

• 批准号: 16406013
• 负责人: KURAZONO Hisao
• 金额: $ 7.23万
• 依托单位: Osaka Prefecture University (2005)Okayama University (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16406013/
• 关键词: Southeast Asia; Bacteria; Intestinal Infection; Virulence factor; Epidemiology; 腸管感染症

A gene encoding the resuscitation-promoting factor (Rpf) from Salmonella Typhimurium LT2 was cloned and characterized. The S.Typhimurium LT2 rpf gene is 696 bp long, and shared a conserved segment with Salmonella enterica serovar Oranienburg (99.4%). The rRpf not only promoted proliferation but also induced resuscitation of VNC (viable but non-culturable) cells to the culturable state in a dose-dependent manner. Therefore, rRpf may be useful for detection of bacterial contaminants present in the VNC form in food samples and the environment (Panutdaporn N. et al, Int.J.Food Microbiol. 106 : 241-247, 2006).In an enterohemorrhagic Escherichia coli (EHEC) O157:H7 outbreak caused by salted salmon roe that occurred in Japan, 1998, a food isolate (F2) was NaCl-resistant and a patient isolate (P5) was sensitive to NaCl. Hydrogen peroxidase, like NaCl, induced a significant loss of culturability in P5. The BacLight assay suggested that the EHEC O157:H7 entered a viable but non-culturable (VNC) state. Mouse-passaged isogenic variants of F2 became NaCl- and oxidation-sensitive, entered the non-culturable state in response to either of these stresses, and could be resuscitated by sodium pyruvate. In vivo passage negatively modulated RpoS expression, and the subsequent stress exposure induced the VNA state in the EHEC O157:H7 isolates (Asakura H. et al, FEMS Microbiol.Lett. 253 : 243-249, 2005).

从鼠伤寒沙门氏菌LT 2中克隆了复苏促进因子（Rpf）的编码基因。鼠伤寒沙门氏菌LT 2 rpf基因长696 bp，与肠道沙门氏菌Oranienburg血清型（99.4%）有一个保守片段。rRpf不仅促进增殖，而且以剂量依赖性方式诱导VNC（活的但不可培养的）细胞复苏至可培养状态。因此，rRpf可用于检测食品样品和环境中以VNC形式存在的细菌污染物（Panutdaporn N.等人，Int.J.Food Microbiol.一百零六：在1998年日本发生的由盐腌鲑鱼卵引起的肠出血性大肠杆菌（EHEC）O 157：H7爆发中，食物分离物（F2）是NaCl抗性的，而患者分离物（P5）对NaCl敏感。过氧化氢酶，如NaCl，诱导P5的可培养性的显着损失。BacLight检测表明，EHEC O 157：H7进入活但不可培养（VNC）状态。小鼠传代的F2的同基因变异体成为NaCl和氧化敏感，进入非培养状态，以响应这些压力，并可以复苏丙酮酸钠。体内传代负调节RpoS表达，随后的应激暴露诱导EHEC O 157：H7分离株中的VNA状态（Asakura H.等人，FEMS Microbiol.Lett. 253：243-249，2005）。

项目成果

Subtyping of uropathogenic Escherichia coli according to the pathogenicity island encoding uropathogenic-specific protein:: Comparison with phylogenetic groups

• DOI: 10.1111/j.1442-2042.2006.01398.x
• 发表时间: 2006-06-01
• 期刊: INTERNATIONAL JOURNAL OF UROLOGY
• 影响因子: 2.6
• 作者: Kanamaru, Sojun;Kurazono, Hisao;Yamamoto, Shingo
• 通讯作者: Yamamoto, Shingo

Functional antagonism between Helicobacter pylori CagA and VacA in control of the NFAT signaling pathway in gastric epitherial cells.

幽门螺杆菌 CagA 和 VacA 之间的功能拮抗作用控制胃上皮细胞中的 NFAT 信号通路。

• 发表时间: 2005
• 期刊: Proc.Natl.Acad.Sci.USA. 102
• 作者: Yokoyama;K.;Hirayama;T.et al.
• 通讯作者: T.et al.

Inhibitory effects of polyphenols on gastric injury by Helicobacter pylori VacA toxin

多酚对幽门螺杆菌VacA毒素胃损伤的抑制作用

• 发表时间: 2005
• 期刊: Helicobacter 10
• 作者: Yahiro K;et al.
• 通讯作者: et al.

Isolation of mini-Tn5Km2 insertion mutants of Salmonella enterica Serovar Oranienburg sensitive to NaCl-induced ostotic stress.

对 NaCl 诱导的骨应激敏感的肠沙门氏菌奥拉宁堡血清型微型 Tn5Km2 插入突变体的分离。

• 发表时间: 2004
• 期刊: Microbiol.Immunol. 48
• 作者: Asakura;H.;Makino;S.et al.;Ogushi K. et al.;Asakura H. et al.
• 通讯作者: Asakura H. et al.

Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin.

• DOI: 10.1016/j.ijmm.2004.09.012
• 发表时间: 2005-01
• 期刊: International journal of medical microbiology : IJMM
• 作者: A. Wada;Ai-ping Wang;H. Isomoto;Y. Satomi;T. Takao;A. Takahashi;Shizuka Awata;T. Nomura;Y. Fujii;S. Kohno;K. Okamoto;J. Moss;J. Millán;T. Hirayama