Analysis of bacterial infectious-pathogens in Japan and Asian countries.

日本和亚洲国家的细菌传染性病原体分析。

• 批准号: 13576013
• 负责人: KURAZONO Hisao
• 金额: $ 8.45万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13576013/
• 关键词: Salmonella oranienburg; STEC; Helicobacter pylori; morphine; bead-ELISA; RPTP β; Salmonella Oranienburg; Salmonella enteritidis; タイ王国; インド

Salmonella Oranienburg strains which had isolated from the patient in 1999 were VNC and sensitive to NaCl osmotic stress. After the passage of these strains in mice, they became resistant to NaCl osmotic stress. VNC Salmonella Oranienburg would be potentially dangerous contaminants of NaCl-preserved foods. VNC Salmonella showed high pathogenicity to morphine-treated mice. The morphine-treated mouse is the good bioassay model for VNC Salmonella.We investigated the secretion of STEC from the sheep for 15 months. Once a sheep had STEC, it maintained STEC and secreted it for a long time. A sheep might be important as a reservoir of STEC.We found the homologue of the B-subunit of Cholera toxin and E. coli LT toxin in Citrobacter freundii and determined the sequence of the gene.We constructed the highly sensitive bead-ELISA system for Helicovacter pylori VacA and succeeded to detect VacA directly in the gastric juice of the gastric ulcer patients.The mice deficient in RPTP β do not show mucosal damage by VacA of Helicobacter pylori. This result indicates that erroneous RPTP β signaling induced gastric ulcers.

1999年从患者体内分离到的Oranienburg沙门氏菌为VNC株，对氯化钠渗透胁迫敏感。这些菌株在小鼠身上传代后，它们对氯化钠渗透胁迫产生了抵抗力。VNC橙色沙门氏菌可能是氯化钠腌制食品的潜在危险污染物。VNC沙门氏菌对吗啡处理的小鼠有较强的致病性。吗啡处理的小鼠是VNC沙门氏菌良好的生物测定模型。我们观察了绵羊15个月的STEC分泌情况。绵羊一旦有STEC，就会维持STEC并长期分泌。我们在弗氏柠檬酸杆菌中发现了霍乱毒素B亚基与大肠杆菌LT毒素的同源物，并测定了该基因的序列。构建了幽门螺杆菌VacA的高灵敏微球ELISA检测体系，并成功地从胃溃疡患者的胃液中直接检测到了VacA。这一结果表明错误的RTPβ信号导致了胃溃疡。

项目成果

Karasawa T. et al.: "Clirung and characterization of gene encoding homologues of the Bishlaunt of choazrec toxin and the Eschrichia coli heat-laibile enterotoxin than clinical isolates of Citrobacter freundii and E-coli"Infection and Immunity. 70. 7153-71

Karasawa T.等人：“与弗氏柠檬酸杆菌和大肠杆菌的临床分离株相比，choazrec毒素的Bishlaunt和大肠杆菌耐热肠毒素的Bishlaunt同源物的基因编码和特征”感染和免疫。

Karasawa T. et al.: "Cloning and characterization of gene encoding homologues of the B-subunit of Cholera toxin and the Escherichia coli heat-labile enterotoxin from clinical isolates of Citrobacter freundii and E.coli"Infection and Immunity. 70. 7153-715

Karasawa T. 等人：“从弗氏柠檬酸杆菌和大肠杆菌临床分离株中克隆和鉴定编码霍乱毒素 B 亚基和大肠杆菌不耐热肠毒素同源物的基因”感染和免疫。

Ogushi, K. et al.: "Salmonella enteritidis FilC (flagella filament protein) induces human β-defensin-2(hBD-2)mRNA production by Caco-2 cells"J.Biol.Chem.. 276. 30521-30526 (2001)

Ogushi, K. 等人：“肠炎沙门氏菌 FilC（鞭毛丝蛋白）诱导 Caco-2 细胞产生人 β-defensin-2(hBD-2)mRNA”J.Biol.Chem.. 276. 30521-30526（ 2001）

Suk K.et al.: "Long-term excretion of Shiga toxin-producing Escherichia coli (STEC) and experimental infection of O157 in a sheep"Journal of Veterinary Medical Science. 64. 927-931 (2002)

Suk K.等人：“产志贺毒素大肠杆菌（STEC）的长期排泄和绵羊中 O157 的实验性感染”《兽医医学科学杂志》。

Takahashi, A. et al.: "Production of β-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella structural protein"FEBS Lett.. 508(3). 484-488 (2001)

Takahashi, A. 等人：“肠炎沙门氏菌鞭毛结构蛋白诱导的人结肠上皮细胞产生 β-防御素-2” FEBS Lett.. 508(3) (2001)。