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Russia-Japan Cooperative Survey and Breeding of Wild Mice

俄日合作野生小鼠调查与繁育

基本信息

批准号：
10044222
负责人：
YONEKAWA Hiromichi
金额：
$ 10.56万
依托单位：
Tokyo Organization for Medical Research, The Tokyo Metropolitan Institute of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044222/
关键词：
Wild mice Russia China mtDNA polymorphism Karyotype analysis Hemoglobin β chain variants specimen Inbreeding 型解析遺伝的多型亜種分化ヘモグロビン mtDNA ネパールカザフスタン

项目摘要

Collaborating mainly with Russian scientists, we collected about 400 individuals of wild mice from 18 localities in Russia. All of these mice were subjected by morphological (visual observation and measurement of bodies, type specimens with image data), karyological (C-banding pattern and FISH with centromere specific-probes), biochemical (polymorphisms of hemoglobin β chain, complement components, etc.) and genetical analyses (polymorphisms of mitochondrial, ribosomal and microsatellite DNA) to disclose their biological characteristics. Based on the studies, we found that there have been three subspecies of Mus musculus in Russia ; e.g. M.m. musculus, M m. castaneus and M.m. domesticus. M.m. musculus occupied major part of Russia, and M.m. castaneus did only in the Far-eastern coast of Russia. M.m. domesticus is a new member found in the Russia and is distributed near Vladivostok and its Northern area along railway, suggesting that the domesticus subspecies was recetly invaded into Russia from other habitat(s).After the analyses mentioned above, we discovered many mutants including hemoglobin β chain variants, deficiency of complement components and of mast cell activities in the colon, etc. We selected more than 25 breeding pairs including above-mentioned mutants and started to establish them to ve inbred strains at the Miyazaki Medical College (Dr. K.Tsuchiya). Ten strains have passed F5 generations. After their establishment as inbred strains, they will be provided to our mouse community as new biological resources.

我们主要与俄罗斯科学家合作，从俄罗斯18个地方收集了大约400只野生小鼠。所有这些小鼠都接受了形态学（视觉观察和身体测量、带有图像数据的类型标本）、细胞核学（C带模式和带有着丝粒特异性探针的FISH）、生化（血红蛋白β链、补体成分的多态性等）遗传学分析（线粒体、核糖体和微卫星DNA多态性）以揭示其生物学特性。通过研究发现，俄罗斯小家鼠（Mus musculus）有3个亚种，即M.m. musculus、M m. castaneus和M.m.阿提库斯M.M. musculus占据了俄罗斯的大部分，M. m. Castaneus只在俄罗斯远东海岸。M.M.在海参崴及其沿着铁路北方地区发现了一个新成员，表明该亚种是最近从其它栖息地传入俄罗斯的。我们选择了包括上述突变体在内的超过25对育种对，并开始在宫崎医学院（K.Tsuchiya博士）建立它们以获得近交系。有10个菌株通过了F5代。在建立近交系后，它们将作为新的生物资源提供给我们的小鼠社区。