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Distribution of drebrin containing synapses in the brain

含有突触的drebrin在大脑中的分布

基本信息

批准号：
10044237
负责人：
SHIRAO Tomoaki
金额：
$ 1.34万
依托单位：
Gunma University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1998
资助国家：
日本
起止时间：
1998 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044237/
关键词：
drebrin spine neuron cyto keleton actin synapse development aging

项目摘要

1. We have systematically analyzed the Alzheimer's brain using biochemical and histol6gical technique. Dr.Hatanpaa demonstrated that drebrin expression was downregulated more specifically than other synaptic functional proteins. He also showed that drebrin expression decreased in parallel with normal human aging. Drs.Shirao and Sekino analyzed distribution of drebrin containing synapses in the rat brain during development. Wistar rats of various developmental stages from postnatal day 0 to day 20 were transcardially perfused with 3.7% formalin and the brain was removed, postfixed, and their cryostat section was immunostained with ABC method or with immunofluorescence. Before postnatal by 10 drebrin remain to be observed in cell body, axons and dendrites. In contrast at postnatal day 14 drebrin was localized in the dendritic spines, and was not observed in cell soma.2. Then we examined whether this developmental change in drebrin distribution was observed in primary cultured neurons or not. At 7 days in culture, drebrin was observed in neurites, and its staining pattern is continuous, In the cell soma drebrin was concentrated in cortical cytoplasm. Although at this developmental stage spine formation was started, drebrin clusters were not yet observed. At 14 days in culture drebrin staining pattern was most discontinuous and patchy although thin neurites was continuously immunostained. At 21 days in culture all drebrin-staining pattern was patchy. Double immunostaining with synaptophysin and drebrin antibodies demonstrated that drebrin is accumulated in the dendritic spines. Taken together it was demonstrated that drebrin accumulation was occurred in parallel with the maturation of synaptic function, and was indicated that this accumulation was regulated by neuronal activity.

1.我们采用生物化学和组织学技术对阿尔茨海默病的脑组织进行了系统的分析。Hatanpaa博士证明，与其他突触功能蛋白相比，dreplastin的表达下调更为特异。他还表明，dreplastin的表达与正常人的衰老平行下降。Shirao博士和Sekino博士分析了发育过程中大鼠大脑中含有神经元的突触的分布。用3.7%福尔马林灌流出生后0 ~ 20天不同发育阶段的Wistar大鼠，取脑后固定，冰冻切片用ABC法或免疫荧光法进行免疫染色。生后10天以前，在胞体、轴突和树突中仍能观察到树突。而在出生后14天，树突棘定位于树突棘，而细胞体中未观察到.然后，我们检查是否在原代培养的神经元中观察到这种发育性的变化。培养7 d时，神经突起内可见染色质，染色连续，细胞索马胞体内染色质集中于皮层细胞质内。虽然在这个发育阶段脊柱的形成开始，drepletum集群尚未观察到。在培养14天，尽管薄的神经突被连续免疫染色，但染色模式是最不连续和斑片状的。在培养21天时，所有drebrin染色模式均为斑片状。突触素和dreplastin抗体的双重免疫染色表明，dreplastin积累在树突棘。总之，证明了突触功能的成熟与突触积累平行发生，并且表明这种积累受神经元活动的调节。