魚類のトリアシルグリセロール代謝の制御機構に関する研究

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To investigate the potential correlations of metabolisms among the internal energy sources, the analyses of the expression patterns of the genes involved in protein and glycogen/glucose metabolisms were further performed using next generation sequencing (NGS).Statistical analyses of the NGS data revealed complete diverse metabolic responses between muscle and liver of medaka. During 4 days of fasting, muscle appears to prefer to consume protein and glycogen for the energy required by the metabolic processes, whereas liver appears to expense protein, glycogen and TAG reserves. During 8 days of fasting, the TAG metabolic activity would be induced in the muscle, while the consumption of glycogen would be reduced. In contrast, liver would reduce the expenditure of TAG, protein and glycogen reserves. Re-feeding would induce the metabolic levels of TAG and carbohydrate resources and restoration of protein in medaka muscle. In contrast, the restoration of TAGs and expenditure of glycogens would occur in the livers of re-fed medaka.Among the metabolic processes, the variation of TAG transport regulated by lipoprotein lipase (LPL) appears to be closely related to those of TAG levels in both tissues. Therefore, the construction of a medaka LPL expression vector was performed to further investigate the function of LPL on TAG accumulation. Unfortunately, the zebrafish embryos microinjected with constructed plasmid showed no green fluorescence. To investigate the quality and efficiency of constructed LPL expression vector, transfection will be performed with ZF4 cell line.