The role of HP1 alpha dimerisation in maintaining chromatin structure

HP1 α二聚化在维持染色质结构中的作用

• 批准号: DP210102984
• 负责人: A/Prof Elizabeth Hinde
• 金额: $ 28.33万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210102984
• 关键词: role; HP1; alpha; dimerisation; maintaining

Heterochromatin protein 1 alpha (HP1a) is an architectural protein that decorates three-dimensional genome organisation and through self-association into HP1a dimers regulates global gene expression. While there is extensive biochemical evidence on how HP1a molecules bind DNA, dimerise and bridge nucleosomes close together, we still do not know how HP1a regulates higher order chromatin structure in the context of a living cell. Thus, by use of cutting-edge fluorescence microscopy methods, the overall aim of this research project is to determine the biophysical mechanism by which the HP1a monomer to dimer transition spatially and temporally modulates live cell chromatin network organisation to ensure faithful transmission of the genome.

异染色质蛋白1 α (HP1a)是一种修饰三维基因组组织的结构蛋白，并通过与HP1a二聚体的自结合调节全球基因表达。虽然有广泛的生物化学证据表明HP1a分子如何结合DNA，二聚体和桥接核小体紧密相连，但我们仍然不知道HP1a如何在活细胞中调节高阶染色质结构。因此，通过使用尖端的荧光显微镜方法，本研究项目的总体目标是确定HP1a单体到二聚体在空间和时间上转变的生物物理机制，调节活细胞染色质网络组织，以确保基因组的忠实传递。