Tracking DNA repair dynamics in the nuclear landscape of a living cell

追踪活细胞核景观中的 DNA 修复动态

• 批准号: FT200100401
• 负责人: A/Prof Elizabeth Hinde
• 金额: $ 62.12万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT200100401
• 关键词: Tracking; DNA; repair; dynamics; nuclear

This project aims to track DNA repair factor recruitment in the nuclear landscape of a living cell and quantify the role of nucleus architecture in maintenance of genome integrity. By coupling advanced fluorescence microscopy with a novel DNA double strand break inducible cell system, this project expects to uncover how the nucleus spatially coordinates DNA damage detection, assessment and repair in real time. This research is important because DNA damage threatens organism survival and this project has the potential to define how this genomic threat is resolved at the single molecule level. The benefit of this research is a fundamental insight into DNA repair biology and development of imaging technology to quantify genome function.

该项目旨在跟踪活细胞核景观中DNA修复因子的募集，并量化细胞核结构在维持基因组完整性中的作用。通过将先进的荧光显微镜与一种新型的DNA双链断裂诱导细胞系统相结合，该项目有望揭示细胞核如何在空间上协调DNA损伤的真实的检测、评估和修复。这项研究很重要，因为DNA损伤威胁着生物体的生存，该项目有可能确定如何在单分子水平上解决这种基因组威胁。这项研究的好处是对DNA修复生物学和成像技术的发展的基本见解，以量化基因组功能。