A fast fluorescence lifetime imaging microscope to track protein dynamics

用于追踪蛋白质动态的快速荧光寿命成像显微镜

• 批准号: LE210100046
• 负责人: A/Prof Elizabeth Hinde
• 金额: $ 19.3万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE210100046
• 关键词: fast; fluorescence; lifetime; imaging; microscope

This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolution. The benefit of this technology is that it will enable scientists in Australia to image, for the first time, the biophysical mechanism by which a protein navigates intracellular architecture to regulate a complex biological function at the single molecule level.

该项目旨在建立一种快速荧光寿命成像显微镜，可以在活细胞的整个结构框架中跟踪蛋白质的细胞内旅程。通过将单粒子跟踪技术与尖端的荧光寿命相机相结合，这种独一无二的显微镜将使蛋白质的流动性和相互作用能够以前所未有的时间分辨率进行空间映射。这项技术的好处是，它将使澳大利亚的科学家能够首次成像蛋白质导航细胞内结构以在单分子水平上调节复杂生物功能的生物物理机制。