Micro Breath Biomarker Analyzer

项目摘要 这一阶段的SBIR将开发一种微型制造的呼吸生物标志物分析仪。有一个未满足的人 需要易于使用、经济高效的微型设备，以便进行快速、特定和灵敏的分析 呼吸生物标志物，特别是挥发性有机化合物(VOCs)。拟议的分析器是 建立在高性能MEMS(微电子机械系统)气相色谱(GC)基础上 柱子。初步研究表明，MEMS柱具有较高的分离效率和分离效率 速度，并开发了单MEMS一维微型GC的功能样机 纵队。在这一阶段的研究中，将采用MEMS柱技术来开发一种 微细加工的综合二维GC(Micro GCxGC)，从而显著 提高微型GC分离能力，解决呼气VOC需求 生物标志物/剖面分析。第一阶段的具体目标是创建一个原型采样设备，用于 用两个MEMS柱萃取和预富集呼气VOCs的GCxGC 在台式GC上对呼气VOCs进行正交选择，并创建概念验证 所提出的基于MEMS柱的呼气VOC分析仪的原型。 拟议的产品旨在解决对呼气生物标志物的需求未得到满足的问题 学习。它有望在个人暴露评估和健康方面有广泛的应用 状态监控。

Project Summary This Phase I SBIR will develop a microfabricated breath biomarker analyzer. There is an unmet need for easy-to-use, cost-effective, miniature devices for rapid, specific, and sensitive analysis of breath biomarkers, especially volatile organic compounds (VOCs). The proposed analyzer is built on high-performance MEMS (microelectromechanical systems) gas chromatography (GC) columns. A preliminary study demonstrated the MEMS column’s high separation efficiency and speed, and developed a functional prototype of one-dimensional micro GC with a single MEMS column. In this Phase I study, the MEMS column technology will be adopted to develop a microfabricated comprehensive two-dimensional GC (micro GCxGC), so as to significantly improve the separation power of micro GC and address the need for breath VOC biomarker/profile analysis. Phase I specific aims are to create a prototype sampling device for extraction and preconcentration of breath VOCs, demonstrate GCxGC with two MEMS columns having orthogonal selectivity for breath VOCs on a benchtop GC, and create a proof-of-concept prototype of the proposed MEMS column based breath VOC analyzer. The proposed product aims to address the unmet need for point-of-need breath biomarker studies. It is expected to have wide applications for personal exposure assessment and health status monitoring.