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Enhancing bioremediation of groundwater co-contaminated by chlorinated volatile organic compounds and 1,4-dioxane using novel macrocyclic materials

使用新型大环材料增强氯化挥发性有机化合物和 1,4-二恶烷共同污染的地下水的生物修复

基本信息

批准号：
10154239
负责人：
Yuexiao Shen
金额：
$ 29.16万
依托单位：
FLORIDA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-01 至 2025-10-31
项目状态：
未结题

项目摘要

Project Summary The project addresses a common challenge in the remediation of groundwater contaminated with chlorinated volatile organic compounds (CVOCs) and 1,4-dioxane. CVOCs include chlorinated solvents, such as trichloroethylene (TCE) and 1,1,1-trichloroethane (1,1,1-TCA), and their degradation products. Many CVOCs and 1,4-dioxane are known or potential human carcinogens and on the Substance Priority List (SPL) for Superfund sites. CVOCs bioremediation under anaerobic conditions (i.e. reductive dechlorination) is well established. However, bioremediation of mixtures of CVOCs and 1,4-dioxane is not yet feasible due to at least the following three obstacles: 1) low biodegradability of 1,4-dioxane at environmentally relevant concentrations, 2) requirement for aerobic conditions for 1,4-dioxane metabolism but anaerobic conditions for most CVOCs metabolism, and 3) inhibition of 1,4-dioxane biodegradation by CVOCs. This project proposes the following combined remediation approach to address these challenges: first, an innovative macrocyclic material approach to selectively adsorb CVOCs and promote the growth of dechlorinating biofilm on the material surface to anaerobically biodegrade CVOCs. After the CVOCs treatment, another type of innovative macrocyclic material as an effective and selective sorbent for 1,4-dioxane sustains biofilms consisting of a highly efficient culture to aerobically metabolize 1,4-dioxane. The macrocyclic molecules, which comprise repeating cyclic oligomers with unique geometry and internal chemistry, form specific host-guest complexes with only selected guest molecules (i.e., 1,4-dioxane or CVOCs). A highly efficient 1,4-dioxane-metabolizing culture (previously established) is much more effective at low, environmentally relevant concentrations compared to all others reported in literature. To understand the mechanisms of how the novel sorbents enhance bioremediation and to demonstrate the feasibility of the proposed remediation approach, the researchers will conduct the following work: 1) Computational study, synthesis, and characterization of novel macrocyclic materials. Two sorbents, one that selectively and reversibly adsorbs CVOCs and another that selectively adsorbs 1,4-dioxane will be optimized for use in the bioremediation studies. 2) Mechanistic study of the highly efficient 1,4-dioxane-metabolizing culture. Key microorganisms responsible for the high affinity to 1,4-dioxane in the mixed culture will be isolated and investigated for their degradation intermediates, pathways, and kinetics. 3) Elucidation of interactions among contaminants, microbial cultures, and the novel sorbents. To achieve this, completely mixed flow experiments will be performed, and they will be coupled with mathematical modeling that incorporates phenomena of both sorption and biodegradation in biofilms. 4) Proof-of-concept column studies for bioremediation of CVOCs and 1,4-dioxane mixtures. Two long-term column studies will be performed: ex situ treatment of 1,4-dioxane and in situ bioremediation of CVOCs and 1,4-dioxane mixture in series. Performance objectives will be Maximum Contaminant Levels for CVOCs and the Health Advisory Level for 1,4-dioxane (0.35 µg/L).

项目总结