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TempO-LINC high throughput, high sensitivity single cell gene expression profiling assay

TempO-LINC 高通量、高灵敏度单细胞基因表达谱分析

基本信息

批准号：
10156786
负责人：
BRUCE E. SELIGMANN
金额：
$ 40.23万
依托单位：
BIOSPYDER TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-15 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10156786
关键词：
Address Archives Bar Codes Benchmarking Biological Assay Biological Markers Cell Line Cells Cellular Assay Complementary DNA Coupling Crosslinker Data Disease Dose Gene Expression Gene Expression Profiling Gene Fusion Genes Genomics Goals Human In Situ In Situ Hybridization Ligase Ligation Measurement Measures Methods Molecular Mouse Cell Line Mus Noise Normal Statistical Distribution Nucleotides Oligonucleotides Pathway interactions Performance Peripheral Blood Mononuclear Cell Phase Polyadenylation Process Protocols documentation RNA RNA Splicing Research Personnel SELL gene Sampling Signal Transduction Single Nucleotide Polymorphism Sorting - Cell Movement Species Specificity Specificity Sulfhydryl Compounds Sum Suspensions Time Tissues Transcript Untranslated RNA Variant adduct base cell preparation combinatorial cost crosslink data quality detector differential expression drug development drug efficacy experimental study improved innovation medication safety novel prevent programs response safety assessment single cell sequencing tool transcriptome transcriptome sequencing translational medicine

项目摘要

Summary/Abstract: Single cell gene expression assays have become important tools to identify functional subtypes of cells, as well as changes within specific subtypes resulting from diseases or treatments. However, most current methods require dedicated hardware and kits, making them expensive, and most are 3’ based and thus cannot measure splice variants, gene fusions, expressed single nucleotide variants, or RNAs that are not polyadenylated. Furthermore, there is no method to-date that enables investigators to measure low or even many moderately expressed genes from single cells, which prevents measurements of many key biomarkers and important genes in molecular pathways and functions. Current methods also only measure a limited number of genes/cell and do not provide quantitative measurements of the abundance of those genes, and thus cannot be used to measure changes in expression level along the order of magnitude possible using bulk preparations of cells, nor can they be used to carry out dose response experiments at the single cell level. Our approach will enable investigators to carry out single cell assays without purchase of proprietary hardware, and provide a level of performance comparable to the profiling of bulk cell samples. Based on the commercial targeted gene expression TempO-Seq® bulk cell assay, we will implement a TempO-LINC single cell assay which, based on preliminary data, will provide data measuring the same genes (low, moderate, high expressed) and similar number of genes/cell as can be measured from bulk samples, quantitatively, providing a similar dynamic expression range and normal distribution of counts as measured from bulk samples. TempO-LINC can be used to process a few 100 single cells up to 100,000+ cells at a time. TempO-LINC data will be benchmarked against both bulk cell data and 10x Genomics single cell data. Furthermore, we will demonstrate utility to measure splice variants and to provide single cell dose response data. These data will be generated using the commercial S1500 surrogate whole transcriptome assay adapted to the TempO-LINC process. In a follow-on Phase II program, the human and mouse whole transcriptome assays will be implemented, the TempO-LINC assay commercialized, and additional applications demonstrated using cell lines, purified cells, and cells dissociated from tissues. The performance and capabilities of the TempO-LINC single cell assay will drive expansion of applications pursued within the single cell field beyond identification of subpopulations of cells.

摘要/摘要：单细胞基因表达检测已成为鉴定细胞功能的重要工具