Current Trends in Stem Cell Therapies

干细胞疗法的当前趋势

• 批准号: 10158506
• 负责人: Sham S. Kakar
• 金额: $ 22.69万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10158506
• 关键词: stem cell therapy; trend

PROJECT SUMMARY/ABSTRACT Stem cell biology has become one of the most exciting scientific areas of investigation. Stem cells are a key to understanding: i) the physiology of tissue rejuvenation, ii) organ regeneration after injury and iii) the regulation of the lifespan. A looming educational challenge is the preparation of young investigators to engage in research on these very important cells in the body. The focus of this training program “Current Trends in Stem Cell Therapies (CTSCT)” Training Program is to understand the role of stem cells in hematology and tissue injury. The training program is derived from the experience of mentoring faculty and the strength of clinical utilization of adult stem cells isolated from postnatal tissues in stem cell processing laboratories employed in the Bone Marrow Transplant Unit, Department of Cardiology, and the Department of Pediatrics at the University of Louisville (UofL). This grant will involve the post-doctoral fellows by engaging them in conceptual laboratory work aimed at the optimization of the: i) isolation of versatile populations of stem cells from adult issues, ii) their ex vivo expansion alone, or in combination with synthetic scaffolds iii) studies on the role of paracrine-related effects of stem cells in regeneration, with special emphasis on extracellular microvesicles (ExMV) and iv) strategies of stem cell delivery to damaged organs including mechanism orchestrating their homing and engraftment. The goals of our training program (CTSCT) will be achieved with a multidisciplinary approach that includes the dedicated faculty from various departments, centers, institutes, and schools. The highly experienced and multidisciplinary faculty at the UofL will provide critical resources and expertise in performing cutting-edge basic and clinical research in this field. This proposal is an innovative postdoctoral training program that will serve as a platform for developing interdisciplinary and integrated approaches that will shed more light on the stem cell compartment under steady-state and pathological conditions. The proposed training program will support five postdoctoral trainees. Two of them will be recruited in year one and then three more in year two to maintain a total of 5 trainees. These may be existing postdoctoral fellows who have matriculated at UofL, or candidates selected by an open recruitment from highly reputed research institutions. This training program will also serve as an important vehicle to integrate the stem cell research performed at UofL. This will be achieved by organizing integrated stem cell-related special courses, seminars, lectures, journal clubs, and open discussions to exchange new ideas among postdoctoral fellows participating in this program and with other students and faculty members at UofL. In addition, the teams of mentors (including both basic and clinical scientists) will promote the importance of multidisciplinary training and the translation of basic science research findings to the community of Kentucky. In summary, our CTSCT training program will effectively facilitate multidisciplinary approaches to studying stem cell biology and is consistent with the NIH Roadmap and the strategic plans of the NHLBI and the overall developmental goals of the UofL.

项目摘要/摘要 干细胞生物学已经成为最令人兴奋的科学研究领域之一。干细胞是一把钥匙 理解：i)组织再生的生理学，ii)损伤后的器官再生，iii)调节 寿命的长短。一个迫在眉睫的教育挑战是年轻研究人员的准备工作 对人体内这些非常重要的细胞进行研究。本培训项目的重点是《茎的最新趋势》 细胞疗法(CTSCT)“培训计划旨在了解干细胞在血液学和组织学中的作用 受伤。培训计划源于指导教师的经验和临床的实力 从出生后组织中分离的成体干细胞在干细胞处理实验室中的利用 骨髓移植单位，心脏内科，儿科 路易斯维尔大学(UofL)。这项资助将使博士后研究员参与到概念性研究中来 旨在优化的实验室工作：i)从成人中分离多种干细胞群体 问题，二)单独进行体外扩张，或与合成支架联合使用三)研究 干细胞在再生中的旁分泌相关作用，特别是细胞外微泡 (ExMV)和IV)将干细胞运送到受损器官的战略，包括协调其 归位和嫁接。我们的培训计划(CTSCT)的目标将通过多学科的合作来实现 这种方法包括来自不同部门、中心、研究所和学校的敬业教师。这个 UofL经验丰富的多学科教员将在以下领域提供关键资源和专业知识 在该领域开展前沿的基础和临床研究。这项建议是一位创新性的博士后 培训计划，将作为开发跨学科和综合方法的平台， 将对稳态和病理条件下的干细胞室提供更多的信息。这个 拟议的培训计划将支持5名博士后实习生。他们中的两人将在第一年和 然后在第二年再增加3名，共维持5名学员。这些可能是现有的博士后研究员 已被UofL录取，或通过公开招聘从知名研究机构中挑选出候选人 机构。这一培训计划也将成为整合干细胞研究的重要载体。 在UofL执行。这将通过组织与干细胞有关的综合特别课程、研讨会、 讲座、期刊俱乐部和公开讨论，以在参与的博士后研究员之间交流新想法 在这个项目中，以及与UofL的其他学生和教职员工一起。此外，导师团队 (包括基础和临床科学家)将促进多学科培训的重要性和 将基础科学研究成果翻译给肯塔基州社区。总之，我们的CTSCT培训 该计划将有效地促进多学科方法来研究干细胞生物学，并保持一致 根据NIH路线图和NHLBI的战略计划以及UofL的总体发展目标。

项目成果

Novel view of the adult stem cell compartment - a developmental story of germline and parental imprinting.

成体干细胞区室的新观点——种系和亲代印记的发育故事。

• 发表时间: 2019
• 期刊: Proceedings of stem cell research and oncogenesis
• 作者: Ratajczak,MariuszZ;Domingues,Alison;Suman,Suman;Straughn,AlexR;Kakar,ShamS;Suszynska,Malwina
• 通讯作者: Suszynska,Malwina

Pannexin-1 channel "fuels" by releasing ATP from bone marrow cells a state of sterile inflammation required for optimal mobilization and homing of hematopoietic stem cells.

• DOI: 10.1007/s11302-020-09706-1
• 发表时间: 2020-09
• 期刊: Purinergic signalling
• 影响因子: 3.5
• 作者: Cymer M;Brzezniakiewicz-Janus K;Bujko K;Thapa A;Ratajczak J;Anusz K;Tracz M;Jackowska-Tracz A;Ratajczak MZ;Adamiak M
• 通讯作者: Adamiak M

Overview of follicle stimulating hormone and its receptors in reproduction and in stem cells and cancer stem cells.

• DOI: 10.7150/ijbs.63721
• 发表时间: 2022
• 期刊: International journal of biological sciences
• 影响因子: 9.2
• 作者: Haldar S;Agrawal H;Saha S;Straughn AR;Roy P;Kakar SS
• 通讯作者: Kakar SS

Withaferin A and Ovarian Cancer Antagonistically Regulate Skeletal Muscle Mass.

• DOI: 10.3389/fcell.2021.636498
• 发表时间: 2021
• 期刊: Frontiers in cell and developmental biology
• 影响因子: 5.5
• 作者: Straughn AR;Kelm NQ;Kakar SS
• 通讯作者: Kakar SS

Application of Stem Cell Therapy for Infertility.

• DOI: 10.3390/cells10071613
• 发表时间: 2021-06-28
• 期刊: Cells
• 影响因子: 6
• 作者: Saha S;Roy P;Corbitt C;Kakar SS
• 通讯作者: Kakar SS