Current Trends in Stem Cell Therapies

干细胞疗法的当前趋势

• 批准号: 10158506
• 负责人: Sham S. Kakar
• 金额: $ 22.69万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10158506
• 关键词: stem cell therapy; trend

PROJECT SUMMARY/ABSTRACT Stem cell biology has become one of the most exciting scientific areas of investigation. Stem cells are a key to understanding: i) the physiology of tissue rejuvenation, ii) organ regeneration after injury and iii) the regulation of the lifespan. A looming educational challenge is the preparation of young investigators to engage in research on these very important cells in the body. The focus of this training program “Current Trends in Stem Cell Therapies (CTSCT)” Training Program is to understand the role of stem cells in hematology and tissue injury. The training program is derived from the experience of mentoring faculty and the strength of clinical utilization of adult stem cells isolated from postnatal tissues in stem cell processing laboratories employed in the Bone Marrow Transplant Unit, Department of Cardiology, and the Department of Pediatrics at the University of Louisville (UofL). This grant will involve the post-doctoral fellows by engaging them in conceptual laboratory work aimed at the optimization of the: i) isolation of versatile populations of stem cells from adult issues, ii) their ex vivo expansion alone, or in combination with synthetic scaffolds iii) studies on the role of paracrine-related effects of stem cells in regeneration, with special emphasis on extracellular microvesicles (ExMV) and iv) strategies of stem cell delivery to damaged organs including mechanism orchestrating their homing and engraftment. The goals of our training program (CTSCT) will be achieved with a multidisciplinary approach that includes the dedicated faculty from various departments, centers, institutes, and schools. The highly experienced and multidisciplinary faculty at the UofL will provide critical resources and expertise in performing cutting-edge basic and clinical research in this field. This proposal is an innovative postdoctoral training program that will serve as a platform for developing interdisciplinary and integrated approaches that will shed more light on the stem cell compartment under steady-state and pathological conditions. The proposed training program will support five postdoctoral trainees. Two of them will be recruited in year one and then three more in year two to maintain a total of 5 trainees. These may be existing postdoctoral fellows who have matriculated at UofL, or candidates selected by an open recruitment from highly reputed research institutions. This training program will also serve as an important vehicle to integrate the stem cell research performed at UofL. This will be achieved by organizing integrated stem cell-related special courses, seminars, lectures, journal clubs, and open discussions to exchange new ideas among postdoctoral fellows participating in this program and with other students and faculty members at UofL. In addition, the teams of mentors (including both basic and clinical scientists) will promote the importance of multidisciplinary training and the translation of basic science research findings to the community of Kentucky. In summary, our CTSCT training program will effectively facilitate multidisciplinary approaches to studying stem cell biology and is consistent with the NIH Roadmap and the strategic plans of the NHLBI and the overall developmental goals of the UofL.

项目总结/摘要 干细胞生物学已成为最令人兴奋的科学研究领域之一。干细胞是 理解：i）组织再生的生理学，ii）损伤后的器官再生和iii） 的寿命。一个迫在眉睫的教育挑战是准备年轻的调查人员从事 研究这些人体中非常重要的细胞。本次培训计划的重点是“股骨柄的当前趋势” 细胞疗法（CTSCT）”培训计划是为了了解干细胞在血液学和组织中的作用 损伤该培训计划是来自指导教师的经验和临床的力量 在干细胞处理实验室中利用从出生后组织分离的成体干细胞， 骨髓移植单位，心脏病科，和儿科在 路易斯维尔大学（UofL）。这项赠款将涉及博士后研究员，让他们参与概念 实验室工作旨在优化：i）分离来自成人的多功能干细胞群体 ii）它们单独或与合成支架组合的离体扩增iii）关于 干细胞在再生中的旁分泌相关作用，特别强调细胞外微泡 （ExMV）和iv）干细胞递送至受损器官的策略，包括协调它们的机制 归巢和移植。我们的培训计划（CTSCT）的目标将通过多学科的 方法，包括来自各个部门，中心，研究所和学校的专门教师。的 UofL经验丰富的多学科教师将提供关键资源和专业知识， 在这一领域进行尖端的基础和临床研究。这一建议是一个创新的博士后 培训计划，将作为一个平台，开发跨学科和综合方法， 将在稳态和病理条件下揭示更多的干细胞区室。的 拟议的培训计划将支持5名博士后学员。其中两人将在第一年被招募， 然后在第二年增加三个，以保持总共5名受训人员。这些可能是现有的博士后研究员， 在UofL录取，或候选人通过公开招聘从高度知名的研究选择 机构职能体系这项培训计划也将成为整合干细胞研究的重要工具 在UofL。这将通过组织与干细胞相关的综合特别课程、研讨会、 讲座，期刊俱乐部和公开讨论，以交流博士后研究员之间的新思想， 在这个程序和其他学生和教职员工在UofL。此外，导师团队 （包括基础和临床科学家）将促进多学科培训的重要性， 将基础科学研究成果翻译给肯塔基州的社区。总之，我们的CTSCT培训 该计划将有效地促进多学科的方法来研究干细胞生物学，并符合 与NIH路线图和NHLBI的战略计划和UofL的总体发展目标。

项目成果

Novel view of the adult stem cell compartment - a developmental story of germline and parental imprinting.

成体干细胞区室的新观点——种系和亲代印记的发育故事。

• 发表时间: 2019
• 期刊: Proceedings of stem cell research and oncogenesis
• 作者: Ratajczak,MariuszZ;Domingues,Alison;Suman,Suman;Straughn,AlexR;Kakar,ShamS;Suszynska,Malwina
• 通讯作者: Suszynska,Malwina

Pannexin-1 channel "fuels" by releasing ATP from bone marrow cells a state of sterile inflammation required for optimal mobilization and homing of hematopoietic stem cells.

• DOI: 10.1007/s11302-020-09706-1
• 发表时间: 2020-09
• 期刊: Purinergic signalling
• 影响因子: 3.5
• 作者: Cymer M;Brzezniakiewicz-Janus K;Bujko K;Thapa A;Ratajczak J;Anusz K;Tracz M;Jackowska-Tracz A;Ratajczak MZ;Adamiak M
• 通讯作者: Adamiak M

Overview of follicle stimulating hormone and its receptors in reproduction and in stem cells and cancer stem cells.

• DOI: 10.7150/ijbs.63721
• 发表时间: 2022
• 期刊: International journal of biological sciences
• 影响因子: 9.2
• 作者: Haldar S;Agrawal H;Saha S;Straughn AR;Roy P;Kakar SS
• 通讯作者: Kakar SS

Withaferin A and Ovarian Cancer Antagonistically Regulate Skeletal Muscle Mass.

• DOI: 10.3389/fcell.2021.636498
• 发表时间: 2021
• 期刊: Frontiers in cell and developmental biology
• 影响因子: 5.5
• 作者: Straughn AR;Kelm NQ;Kakar SS
• 通讯作者: Kakar SS

The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation.

• DOI: 10.1007/s12015-022-10481-2
• 发表时间: 2023-01
• 期刊: STEM CELL REVIEWS AND REPORTS
• 影响因子: 4.8
• 作者: Abdelbaset-Ismail, Ahmed;Ciechanowicz, Andrzej K.;Bujko, Kamila;Ratajczak, Janina;Kucia, Magdalena;Ratajczak, Mariusz Z.
• 通讯作者: Ratajczak, Mariusz Z.