Disclipine A - Analytical Track 4: Chemistry Capability/Capacity Development

学科 A - 分析轨道 4：化学能力/能力发展

• 批准号: 10160666
• 负责人: TIMOTHY JOHN HERRMAN
• 金额: $ 13.5万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10160666
• 关键词: Disclipine; Analytical; Track; Chemistry; Capability

Abstract PROJECT FOUR: Capacity Expansion: Texas Whole Genome Sequencing of Isolates from Animal Food Office of the Texas State Chemist (OTSC) has the ability to screen and/or confirm the following organisms: Salmonella spp. in animal food, animal food ingredients, biosolids, human food; Shiga like toxin producing E.coli (STEC) O157:H7 and non-O157 in animal food, animal food ingredients and human food; Listeria monocytogenes in raw milk, animal food and animal food ingredients, human food; SEB in raw milk, Brucella spp. antigen in raw milk, ricin toxin via Tetracore ELISA, Shigella spp. in human food, Bacillus anthracis sterne in human food, Staphylococcal enterotoxins in human food, T-2 toxin and alpha- amanitin by LCMSMS. The microlab received BSL3 training in screening methodologies of Bacillus anthracis and Yersinia pestis at USAMRIID facility, Ft Detrick, MD in 2018 and are moving forward to establish this method (screening and confirmatory) at OTSC facility. The OTSC whole genome sequencing capability development project includes: 1) purchasing MiSeq sequencing platform and necessary instruments, 2) completing and passing competency assessment, 3) expanding the OTSC ISO/IEC 17025.2017 scope to include genome sequencing. As an outcome of the Capacity Expansion: Texas Whole Genome Sequencing of Isolates from Animal Food project, OTSC will begin DNA sequencing using a MiSeq internally within the lab for Listeria and Salmonella DNA, which will strengthen disease surveillance and epidemiology.

摘要 项目四：产能扩张：德克萨斯州分离株全基因组测序 动物食品 德克萨斯州化学家办公室（OTSC）有能力筛选和/或确认以下内容 微生物：沙门氏菌属动物食品、动物食品配料、生物固体、人类食品; 动物性食品中的产滋贺样毒素大肠杆菌（STEC）O 157：H7和非O 157，动物性食品 原料和人类食品;生奶、动物食品和动物食品中的单核细胞增生李斯特菌 成分，人类食品; SEB在生牛奶，布鲁氏菌属。原料奶中的抗原，蓖麻毒素， Tetracore ELISA，志贺氏菌属在人类食物中，炭疽杆菌在人类食物中存在， 人类食物中的葡萄球菌肠毒素、T-2毒素和α-鹅膏蕈碱（LCMSMS）。的 microlab接受了炭疽杆菌和耶尔森氏菌筛查方法的BSL 3培训 鼠疫在USAMRIID设施，Ft Detrick，MD在2018年，并正在向前迈进，以建立这一 方法（筛选和确认）在OTSC机构。OTSC全基因组测序 能力开发项目包括：1）购买MiSeq测序平台， 必要的工具，2）完成并通过能力评估，3）扩大 OTSC ISO/IEC 17025.2017范围包括基因组测序。的成果是 产能扩张：德克萨斯州动物食品分离株全基因组测序项目， OTSC将开始在实验室内部使用MiSeq进行李斯特菌DNA测序， 沙门氏菌DNA，这将加强疾病监测和流行病学。