Discipline A: Microbiology, Analytical Track 2: Human Food Product Testing

项目摘要/摘要 - 微生物学： 分析轨道 #2：人类食品测试： 国家卫生实验室（SHL）参与该项目有助于实现该项目的总体目标 通过为 FDA 和 SHL 建立的基于风险和预防为主的食品安全系统做出贡献来提供资助 爱荷华州合作伙伴（爱荷华州监察和上诉部以及爱荷华州公共部门） 生）可以利用。此外，生成的测试结果可用于消除商业中的掺假食品 并协助监管检查计划进行调查。 该项目的具体目标如下： • 通过测试 200 个新鲜农产品样品来参与大型单一商品/危害对分析。 多种病原体：沙门氏菌、志贺毒素大肠杆菌 (STEC) 和环孢菌（其中仅 40 种） 样品进行环孢子虫测试）。 • 测试 FDA 和 SHL 感兴趣的另外两种商品，这些商品最近导致了疫情爆发； 25 种针对沙门氏菌的即食麦片和 25 种针对沙门氏菌、STEC 和李斯特菌的什锦预切甜瓜 物种 • 参加FDA 要求的演习以支持和保持准备状态。 • 参加国家特殊安全活动演习（如有）。 • 参与与可疑或可信的食品供应威胁相关的测试，其中 根据 FDA 的要求，怀疑存在微生物制剂。 该项目的成功完成将有助于 FDA 加强其国家综合食品安全体系 并将加强检测、预防和尽量减少食源性接触的努力，并最终减少 食源性疾病。该项目也将有利于SHL提升其检测能力和能力 大量生产针对各种病原体的产品，向其他工作人员传授这些方法（尤其是 环孢子虫），继续与爱荷华州检验和上诉部就样品进行各种流程合作 收集和监管链在疫情爆发期间至关重要。任何发现的病原体也将被 通过全基因组测序进行 DNA 指纹识别，该数据由伊利诺伊州公共卫生局 (IDPH) 在全州范围内使用，在全国范围内由 FDA 用于快速疫情检测和来源识别，以减少食源性疾病。

Project Summary/Abstract - Microbiology: Analytical Track #2 : Human Food Product Testing: The State Hygienic Laboratory’s (SHL) participation in this project contributes to the overall objective of the grant by contributing to a risk-based and prevention focused food safety system that both FDA and SHL’s State of Iowa partners (Iowa Department of Inspections and Appeals and the Iowa Department of Public Health) can utilize. In addition, test results generated can be used to remove adulterated food from commerce and aide regulatory inspection programs in conducting investigations. The specific aims of this project are as follows: • Participate in the large single commodity/hazard pair analysis by testing 200 fresh produce samples for multiple pathogens: Salmonella, Shiga-toxin Escherichia coli (STEC) and Cyclospora (only 40 of these samples tested for Cyclospora). • Test two other commodities of interest to both FDA and SHL that have contributed to outbreaks recently; 25 ready to eat cereal for Salmonella and 25 assorted precut melons for Salmonella, STEC, and Listeria species • Participate in FDA-requested exercises to support and maintain readiness. • Participate in national special security event exercises, as available. • Participate in testing associated with suspected or credible threats to the food supply where a microbiological agent is suspected, as requested by FDA. Successful completion of this project will help the FDA enhance its nationally integrated food safety system and will strengthen its efforts to detect, prevent, and minimize foodborne exposures and ultimately reduce foodborne illness. This project will also be beneficial to SHL to improve its capability and capacity for testing large volumes of produce for a variety of pathogens, teach additional staff members these methods (especially Cyclospora), continue to work various processes with Iowa Department of Inspection and Appeals on sample collection and chain of custody which is crucial during outbreaks. Any pathogens that are found will also be DNA fingerprinted by whole genome sequencing and this data utilized statewide by IDPH and nationally by FDA for rapid outbreak detection and source identification to reduce foodborne illness.