The ShEEP request for NanoString GeoMx Digital Spatial Profiling and nCounter MAX Complete System

该ShEEP申请要求资金购买完整的NanoString GeoMx®数字系统 Spatial Profiling和nCounter® MAX将用于支持弗吉尼亚州中部的VA研究 卫生保健系统。九个主要的VA资助的研究人员，包括周博士，海勒蒙，帕特尔，王， Bajaj、Ren、潘达克、Gartenhaus和Ghosh需要这种设备来测量mRNA、miRNA、lncRNA 以及细胞、新鲜或冷冻组织、血清和胆汁中的蛋白质表达，以获得他们资助的VA优异奖 和NIH赠款。所要求的NanoString的GeoMx ®数字空间轮廓技术可以检测和 使用抗体或RNA以显著更高的空间分辨率定量蛋白质和RNA 与可光裂解的DNA寡核苷酸结合的探针，可通过nCounter® MAX读数进行非原位定量 从福尔马林固定石蜡包埋（FFPE）或新鲜冷冻的特定感兴趣区域（ROI） 组织.可变ROI选择允许对直径为700 μm的区域进行分析，直至单个细胞（10- 200 μ m）。 µm）。不需要第二抗体或扩增试剂。GeoMx® Digital的读数 Spatial Profiler由全自动且易于使用的nCounter® MAX分析系统执行。 nCounter® MAX提供了在不到24小时内经济高效地完成项目所需的一切 小时比qPCR更准确、可重复和高度多重化，更简单、更快速、更 nCounter® MAX比NGS更具成本效益，可以在一个特定的环境中对多个路径进行数字检查。 在一个无需提取的工作流程中，只需15分钟的动手时间。这允许 核心设施，通过减少样品制备时间和执行自我驱动， 使用开放式nSolver®分析软件进行简单、快速的数据分析，这是一个很好的工具， 协同工作。直观的工作流程，从样品到数据只需15分钟的动手时间。 单独的数字分析仪和制备站单元有助于消除样品处理中的瓶颈， 数据收集。不需要专门的生物信息学家。直接数字计数生成的结果， 报告在一个标准的CSV文件，可以导入到任何喜爱的应用程序或使用包括 nSolver®分析软件。企业软件包包括实验室， 增强安全性。控制用户访问、自动化数据流并生成审计日志。当前没有任何 我们的VA研究机构也有类似的设备。调查人员依靠外界 合同服务，这不仅是昂贵和耗时的，而且还需要生物信息学的支持。 鉴于分子变化（RNA、DNA、miRNA和蛋白质表达）在生物学中的关键作用， 在整个发育和疾病过程中，这种设备将使我们的研究人员能够促进 现有的研究和追求未探索的研究领域的效率和准确性，不能 通过研究设施中的现有仪器实现。因此，我们向ShEEP申请资金， 购买该系统用于我们正在进行的VA资助的与代谢性肝病相关的研究， 是退伍军人事务部研究使命的目标区域。收购这一最先进的系统将大大 扩大我们在基础研究、转化研究和临床研究方面的核心能力， VA资助的基础研究科学家和医生科学家之间的合作。此外，它将 为支持初级研究人员提供一个极好的平台，以生成可靠的初步数据， 争取外部赠款。成功完成这些VA资助的研究不仅将 这不仅有利于美国退伍军人的医疗保健，也有利于所有美国人。

This ShEEP application requested funds to purchase a complete system of NanoString GeoMx® Digital Spatial Profiling and nCounter® MAX that will be used to support VA research at the Central Virginia VA Health Care System. Nine major VA-funded investigators, including Drs. Zhou, Hylemon, Patel, Wang, Bajaj, Ren, Pandak, Gartenhaus, and Ghosh require this equipment to measure mRNA, miRNA, lncRNA and protein expression in cells, fresh or frozen tissues, serum and bile for their funded VA Merit Awards and NIH grants. The requested NanoString’s GeoMx ® Digital Spatial Profiling technology can detect and quantify proteins and RNA at significantly higher plex with spatial resolution using antibodies or RNA probes conjugated to photo-cleavable DNA oligos that are quantified ex situ with nCounter® MAX readout from specific regions of interest (ROI) on Formalin-Fixed Paraffin-Embedded (FFPE) or Fresh Frozen tissue. Variable ROI selection permits the profiling of 700-μm diameter areas down to single cells (10- µm). No secondary antibodies or amplification reagents are required. Readout of the GeoMx® Digital Spatial Profiler is performed by the fully automated and easy-to-use nCounter® MAX Analysis System. The nCounter® MAX provides everything needed to cost-effectively complete projects in less than 24 hours. More accurate, reproducible and highly multiplexed than qPCR and simpler, quicker and more cost-effective than NGS, the nCounter® MAX allows for digital examination of multiple pathways in a single tube in an extraction-free workflow requiring only 15 minutes of hands-on time. This allows the Core facility to accelerate research by spending less time on sample prep and performing self-driven, simple, and rapid data analysis using the open use nSolver® Analysis Software, a great tool for collaborative work. The intuitive workflow with only fifteen minutes of hands-on time from sample to data. Separate Digital Analyzer and Prep Station units help eliminate bottlenecks in sample processing and data collection. No need for a specialized Bioinformaticist. Results generated as direct digital counts and reported in a standard CSV file that can be imported into any favorite application or use the included nSolver® Analysis Software. An enterprise software package is included for laboratories that require enhanced security. Control user access, automate data flow and generate audit logs. Currently, no similar equipment is available at our VA Research facility. The investigators are relying on outside contract services, which are not only costly and time-consuming but also require Bioinformatics’ support. Given the critical role of the molecular changes (RNA, DNA, miRNA and protein expression) in biological processes throughout development and disease, this equipment would allow our investigators to facilitate existing studies and pursue unexplored areas of research with efficiency and accuracy that could not be achieved by the current instrumentation in the research facility. Therefore, we request funds from ShEEP to purchase this system for use in our ongoing VA-funded studies related to metabolic liver diseases that are target areas of the VA research mission. Acquisition of this state-of-the-art system will significantly expand our Core capacity for both basic research, translational and clinical studies and promote collaborations between VA-funded basic research scientists and physician-scientists. Furthermore, it will provide an excellent platform for supporting junior investigators to generate solid preliminary data for competing for external grants. The successful completion of these VA-funded research will not only benefit US Veterans health care but also benefit all Americans.