The ShEEP request for NanoString GeoMx Digital Spatial Profiling and nCounter MAX Complete System

ShEEP 对 NanoString GeoMx 数字空间分析和 nCounter MAX 完整系统的请求

• 批准号: 10161359
• 负责人: HUIPING Rose ZHOU
• 金额: --
• 依托单位: VA VETERANS ADMINISTRATION HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10161359
• 关键词: Alcohols; American; Antibiotic Resistance; Antibodies; Area; Award; Basic Science; Bile fluid; Biochemical; Bioinformatics; Biological; Biological Assay; Biological Process; Biopsy; Caliber; Cardiovascular Diseases; Cells; Cirrhosis; Clinical Investigator; Clinical Research; Clostridium difficile; Collaborations; Colon Carcinoma; Color; Complementary DNA; Computer software; Consumption; Contract Services; Core Facility; DNA; Data; Data Analyses; Data Collection; Detection; Development; Disease; Equipment; Formalin; Freezing; Funding; Grant; Health; Healthcare; Healthcare Systems; Hour; Image; Immunoglobulin Variable Region; Infection; Intuition; Laboratories; Libraries; Liver diseases; Measures; Messenger RNA; Metabolic; MicroRNAs; Mission; Molecular; Morphology; Oligonucleotides; Outcome; Paraffin Embedding; Pathway interactions; Patients; Pharmaceutical Preparations; Physicians; Post-Traumatic Stress Disorders; Productivity; Protein Analysis; Proteins; RNA; RNA Probes; RNA analysis; Reagent; Reporting; Reproducibility; Request for Applications; Research; Research Personnel; Resolution; Role; Running; Sampling; Scanning; Scientist; Secure; Security; Serum; Sheep; Slide; Solid; Specimen; System; Systems Analysis; Technology; Time; Tissues; Tube; United States National Institutes of Health; Variant; Veterans; Virginia; Work; animal tissue; base; clinical investigation; cost; cost effective; digital; experience; experimental study; instrumentation; interest; liver injury; nano-string; non-alcoholic fatty liver disease; protein expression; research facility; research study; tool; transcriptome; translational study; tumor

This ShEEP application requested funds to purchase a complete system of NanoString GeoMx® Digital Spatial Profiling and nCounter® MAX that will be used to support VA research at the Central Virginia VA Health Care System. Nine major VA-funded investigators, including Drs. Zhou, Hylemon, Patel, Wang, Bajaj, Ren, Pandak, Gartenhaus, and Ghosh require this equipment to measure mRNA, miRNA, lncRNA and protein expression in cells, fresh or frozen tissues, serum and bile for their funded VA Merit Awards and NIH grants. The requested NanoString’s GeoMx ® Digital Spatial Profiling technology can detect and quantify proteins and RNA at significantly higher plex with spatial resolution using antibodies or RNA probes conjugated to photo-cleavable DNA oligos that are quantified ex situ with nCounter® MAX readout from specific regions of interest (ROI) on Formalin-Fixed Paraffin-Embedded (FFPE) or Fresh Frozen tissue. Variable ROI selection permits the profiling of 700-μm diameter areas down to single cells (10- µm). No secondary antibodies or amplification reagents are required. Readout of the GeoMx® Digital Spatial Profiler is performed by the fully automated and easy-to-use nCounter® MAX Analysis System. The nCounter® MAX provides everything needed to cost-effectively complete projects in less than 24 hours. More accurate, reproducible and highly multiplexed than qPCR and simpler, quicker and more cost-effective than NGS, the nCounter® MAX allows for digital examination of multiple pathways in a single tube in an extraction-free workflow requiring only 15 minutes of hands-on time. This allows the Core facility to accelerate research by spending less time on sample prep and performing self-driven, simple, and rapid data analysis using the open use nSolver® Analysis Software, a great tool for collaborative work. The intuitive workflow with only fifteen minutes of hands-on time from sample to data. Separate Digital Analyzer and Prep Station units help eliminate bottlenecks in sample processing and data collection. No need for a specialized Bioinformaticist. Results generated as direct digital counts and reported in a standard CSV file that can be imported into any favorite application or use the included nSolver® Analysis Software. An enterprise software package is included for laboratories that require enhanced security. Control user access, automate data flow and generate audit logs. Currently, no similar equipment is available at our VA Research facility. The investigators are relying on outside contract services, which are not only costly and time-consuming but also require Bioinformatics’ support. Given the critical role of the molecular changes (RNA, DNA, miRNA and protein expression) in biological processes throughout development and disease, this equipment would allow our investigators to facilitate existing studies and pursue unexplored areas of research with efficiency and accuracy that could not be achieved by the current instrumentation in the research facility. Therefore, we request funds from ShEEP to purchase this system for use in our ongoing VA-funded studies related to metabolic liver diseases that are target areas of the VA research mission. Acquisition of this state-of-the-art system will significantly expand our Core capacity for both basic research, translational and clinical studies and promote collaborations between VA-funded basic research scientists and physician-scientists. Furthermore, it will provide an excellent platform for supporting junior investigators to generate solid preliminary data for competing for external grants. The successful completion of these VA-funded research will not only benefit US Veterans health care but also benefit all Americans.

此 ShEEP 申请要求资金购买完整的 NanoString GeoMx® Digital 系统 空间分析和 nCounter® MAX 将用于支持弗吉尼亚中部 VA 的 VA 研究 医疗保健系统。退伍军人管理局资助的九名主要研究人员，包括博士。周海莱蒙帕特尔王 Bajaj、Ren、Pandak、Gartenhaus 和 Ghosh 需要此设备来测量 mRNA、miRNA、lncRNA 以及细胞、新鲜或冷冻组织、血清和胆汁中的蛋白质表达，获得 VA 优异奖 和美国国立卫生研究院的资助。所要求的 NanoString 的 GeoMx ® 数字空间分析技术可以检测和 使用抗体或 RNA 以显着更高的空间分辨率定量蛋白质和 RNA 与可光裂解 DNA 寡核苷酸缀合的探针，可通过 nCounter® MAX 读数进行异位定量 来自福尔马林固定石蜡包埋 (FFPE) 或新鲜冷冻的特定感兴趣区域 (ROI) 组织。可变 ROI 选择允许对 700 μm 直径区域小至单个细胞（10- 微米）。不需要二抗或扩增试剂。 GeoMx® Digital 的读数 Spatial Profiler 由全自动且易于使用的 nCounter® MAX 分析系统执行。 nCounter® MAX 提供了在 24 天内经济高效地完成项目所需的一切 小时。比 qPCR 更准确、可重复且高度多重化，并且更简单、更快、更多 nCounter® MAX 比 NGS 更具成本效益，允许对多个路径进行数字化检查 免萃取工作流程中的单管仅需 15 分钟的手动操作时间。这允许 核心设施通过减少样本准备和执行自我驱动的时间来加速研究， 使用开放式 nSolver® 分析软件进行简单、快速的数据分析，这是一个很好的工具 协同工作。从样品到数据的直观工作流程仅需十五分钟的实践时间。 独立的数字分析仪和制备站单元有助于消除样品处理和制备过程中的瓶颈 数据收集。不需要专门的生物信息学家。结果生成为直接数字计数和 以标准 CSV 文件形式报告，该文件可以导入到任何喜爱的应用程序中或使用包含的 nSolver® 分析软件。包含一个企业软件包，供需要的实验室使用 增强安全性。控制用户访问、自动化数据流并生成审核日志。目前，没有 我们的 VA 研究机构也有类似的设备。调查人员依靠外部 合同服务不仅成本高、耗时长，而且需要生物信息学的支持。 鉴于分子变化（RNA、DNA、miRNA 和蛋白质表达）在生物中的关键作用 在整个发育和疾病过程中，该设备将使我们的研究人员能够促进 现有的研究并以无法达到的效率和准确性探索尚未探索的研究领域 通过研究设施中现有的仪器实现。因此，我们向 ShEEP 请求资金 购买该系统用于我们正在进行的 VA 资助的与代谢性肝病相关的研究 是 VA 研究任务的目标领域。购买这一最先进的系统将显着 扩大我们的基础研究、转化和临床研究的核心能力，并促进 VA 资助的基础研究科学家和医师科学家之间的合作。此外，它将 为支持初级研究人员生成可靠的初步数据提供了一个优秀的平台 竞争外部资助。这些由 VA 资助的研究的成功完成不仅将 有利于美国退伍军人的医疗保健，也有利于所有美国人。