Biorepository and Tissue Technology Shared Resource

生物资源库与组织技术共享资源 生物储存库和组织技术共享资源（BTTSR）是生物技术的重要组成部分。 支持翻译研究的基础设施。它建立在一个完善的体制模式之上， 组织采集、处理、储存和多模式（组织学、免疫组织化学和分子学） 通过高度进化的 生物信息系统Alfredo Molinolo是一位拥有40多年人类经验的病理学家， 和实验病理学，是BTTSR的主任。截至2018年1月，生物储存库（BRPT） 现在是一个完整的美国病理学家学院（CAP）认证的资源。根据其自身IRB批准的方案 (IRB#090401），BRPT集成了患者同意、前瞻性采购服务、方案和 标准操作程序设计、组织处理和储存。杰森·西克里克是一名胃肠道外科医生， 负责临床事务的BRPT副主任。Sharmeela Kaushal是BRPT经理， 在NCI和CAP指导方针下的BRPT操作方面。三名协调员负责患者知情同意， 两名组织处理技术人员协助她。组织技术助理主任瓦莱里娅·埃斯特拉达， 管理组织技术共享资源（TTSR）。她是一位训练有素的学术病理学家， 在管理和雇佣政策方面的经验，以及对标准/新型病理学的广泛了解 技术.她管理着四名专门的组织技术员。TTSR服务包括组织固定、处理 切片、石蜡切片、冰冻切片、常规染色、特殊染色、个体化免疫组化（IHC） 免疫荧光（IF）分析、抗体优化和验证、数字成像和数字图像 储存、小鼠表型分析、组织微阵列开发和构建、多重IHC和IF，以及 病理学咨询和培训等服务。自2016年7月以来，TTSR设备库存 随着收购AT2 ScanScope载玻片扫描仪、Gemini H & E 染色仪和Biocare Medical免疫组织化学自动染色仪。在过去的五年里，BTTSR已经 支持了174个用户，包括89个MCC成员和11个来自Salk和Sanford-Burnham的成员 Prebys NCI指定的癌症中心。

BIOREPOSITORY AND TISSUE TECHNOLOGY SHARED RESOURCE ABSTRACT The Biorepository and Tissue Technology Shared Resource (BTTSR) is a critical component of the infrastructure supporting translational studies. It is founded on a well-established institutional model integrating tissue procurement, processing, storage, and multimodal (histologic, immunohistochemical, and molecular) analyses with traditional epidemiologic, clinicopathologic and outcome parameters through a highly evolved bioinformatics system. Alfredo Molinolo, a physician pathologist with more than 40 years’ experience in human and experimental pathology, is the Director of the BTTSR. As of January 2018, the Biorepository (BRPT) is now a full College of American Pathologist (CAP)-accredited resource. Under its own IRB approved protocol (IRB# 090401), the BRPT integrates patient consenting, prospective procurement services, protocol and standard operating procedure design, tissue processing, and storage. Jason Sicklick, a GI surgeon, is the Deputy Director of the BRPT for clinical affairs. Sharmeela Kaushal is the BRPT Manager overseeing operational aspects of the BRPT under NCI and CAP guidelines. Three coordinators for patient consenting and two technicians for tissue processing assist her. Valeria Estrada, the Assistant Director for Tissue Technology, supervises the Tissue Technology Shared Resource (TTSR). She is a well-trained academic pathologist with experience in management and employment policies, and extensive knowledge of standard/novel pathology techniques. She supervises four dedicated histotechnicians. TTSR services include tissue fixation, processing and sectioning, paraffin and frozen, routine stains, special stains, individualized immunohistochemistry (IHC) and immunofluorescence (IF) assays, antibody optimization and validation, digital imaging and digital image storage, mouse phenotyping, tissue microarrays developing and construction, multiplexing IHC and IF, and pathology consultation and training, among other services. Since July 2016, the TTSR equipment inventory has grown with the acquisition of, among other equipment, an AT2 ScanScope slide scanner, a Gemini H&E stainer, and a Biocare Medical immunohistochemistry autostainer. Over the past 5 years, the BTTSR has supported 174 users, including 89 MCC members and 11 members from the Salk and Sanford-Burnham Prebys NCI-designated Cancer Centers.