Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens

项目概要/摘要 生物储存库和组织技术共享资源（BTTSR）的这一申请要求资助 BioStore ™ M60 Cryo-190 ° C液氮（LN 2）系统，用于支持持续采集人体固体 生物标本和长期储存，以供未来研究，这一目标符合NCI指定的 综合摩尔癌症中心（MCC），在加州大学，圣地亚哥。这种自动化 BioStore M60冷冻系统是一种低温储存装置，提供样品储存自动化、冷链 管理，并提高了安全性和可访问性，同时保持液体内的样品保护 氮环境所要求的仪器超越了传统的手动液氮冷冻机， 最高的样品保护，全面的库存管理系统，上级用户安全措施，以及 LIMS连接。BioStore ™ M60冷冻-190 °C系统提供样品完整性、安全性和跟踪 具有完整的21-CFR-11报告能力。 生物标本的充分保存已被证明是获得可靠和可重复结果的关键 在基因组学、转录组学、蛋白质组学、开发PDX模型、免疫分析和许多其他测定中。 依赖于-190 °C储存的生物样本的生物测定可能对瞬时的 温度暴露，因此需要该系统的上级质量控制措施。新的BioStore ™ M60 Cryo-190 ° C系统将确保我们的用户能够获得高质量的人体生物标本， 该系统的自动化还将通过最小化 瞬态温度暴露。BioStore ™ M60 Cryo-190 ° C系统将被定位、管理和 在MCC现有的BTTSR设施中维护。

PROJECT SUMMARY/ABSTRACT This application by the Biorepository and Tissue Technology Shared Resource (BTTSR) requests funding for a BioStore™ M60 Cryo-190°C liquid nitrogen (LN 2) system to support ongoing collection of human solid biospecimen and long-term storage for future research, a goal in line with those of the NCI-designated comprehensive Moores Cancer Center (MCC), at the University of California, San Diego. This automated BioStore M60 Cryo system is a cryogenic storage unit that offers sample storage automation, cold chain management, and improved security and accessibility while maintaining sample protection within a liquid nitrogen environment. The requested instrument goes beyond conventional manual LN2 Freezers with the highest sample protection, comprehensive inventory management systems, superior user safety measures, and LIMS connectivity. The BioStore™ M60 Cryo -190°C System provides sample integrity, security and tracking with availability of Full 21-CFR-11 reporting capability. Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results in genomics, transcriptomics, proteomics, developing PDX models, immunoprofiling and many other assays. Biological assays which depend on -190°C stored biospecimens can be extremely sensitive to transient temperature exposure thus the superior quality control measures of this system are needed. The new BioStore™ M60 Cryo -190°C system will ensure that our users have access to high quality human biospecimens, and the automation of the system will also enhance the integrity of samples during storage and retrieval by minimizing transient temperature exposure. The BioStore™ M60 Cryo -190°C system will be located, administered and maintained in the existing BTTSR facility at the MCC.