Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens

项目摘要/摘要 这项由生物信息库和组织技术共享资源(BTTSR)提出的申请申请 BioStore™M60低温-190C液氮(LN)系统，支持持续收集人体固体 生物质谱学和长期储存用于未来研究，目标与NCI指定的目标一致 位于加州大学圣地亚哥分校的综合摩尔癌症中心(MCC)。这是自动的 BioStore M60低温存储系统是一种低温存储单元，提供样品存储自动化、冷链 管理，提高安全性和可及性，同时保持液体中的样品保护 氮素环境。所要求的仪器超越了传统的手动液氮冷冻机，具有 最高的样品保护、全面的库存管理系统、卓越的用户安全措施，以及 LIMS连接。BioStore™M60低温-190C系统提供样品完整性、安全性和跟踪 具备完整的21-CFR-11报告能力。 充分保存生物标本已被证明是获得可靠和可重复性结果的关键 在基因组学、转录组学、蛋白质组学、开发PDX模型、免疫图谱和许多其他分析中。 依赖于-190°C储存的生物样品的生物检测方法可能对瞬变非常敏感 因此，需要对该系统采取更好的质量控制措施。全新的BioStore™ M60 Cryo-190°C系统将确保我们的用户能够获得高质量的人类生物标本，并且 该系统的自动化还将增强样品在储存和取回过程中的完整性，方法是将 瞬时温度暴露。BioStore™M60低温-190C系统将被定位、管理和 维护在MCC现有的BTTSR设施中。

PROJECT SUMMARY/ABSTRACT This application by the Biorepository and Tissue Technology Shared Resource (BTTSR) requests funding for a BioStore™ M60 Cryo-190°C liquid nitrogen (LN 2) system to support ongoing collection of human solid biospecimen and long-term storage for future research, a goal in line with those of the NCI-designated comprehensive Moores Cancer Center (MCC), at the University of California, San Diego. This automated BioStore M60 Cryo system is a cryogenic storage unit that offers sample storage automation, cold chain management, and improved security and accessibility while maintaining sample protection within a liquid nitrogen environment. The requested instrument goes beyond conventional manual LN2 Freezers with the highest sample protection, comprehensive inventory management systems, superior user safety measures, and LIMS connectivity. The BioStore™ M60 Cryo -190°C System provides sample integrity, security and tracking with availability of Full 21-CFR-11 reporting capability. Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results in genomics, transcriptomics, proteomics, developing PDX models, immunoprofiling and many other assays. Biological assays which depend on -190°C stored biospecimens can be extremely sensitive to transient temperature exposure thus the superior quality control measures of this system are needed. The new BioStore™ M60 Cryo -190°C system will ensure that our users have access to high quality human biospecimens, and the automation of the system will also enhance the integrity of samples during storage and retrieval by minimizing transient temperature exposure. The BioStore™ M60 Cryo -190°C system will be located, administered and maintained in the existing BTTSR facility at the MCC.