Biorepository and Tissue Technology Shared Resource

生物信息库与组织技术共享资源摘要 生物信息库和组织技术共享资源(BTTSR)是 支持翻译研究的基础设施。它建立在完善的制度模式基础上， 组织采购、加工、储存和多模式(组织学、免疫组织化学和分子学) 用传统的流行病学、临床病理和预后参数进行分析 生物信息学系统。Alfredo Molinolo，一位拥有40多年人类研究经验的内科病理学家 和实验病理学是BTTSR的主任。截至2018年1月，生物库(BRPT)为 现在是全美病理学家学院(CAP)认可的资源。根据IRB批准的协议 (IRB#090401)，BRPT将患者同意、预期采购服务、协议和 标准的操作程序设计、组织处理和储存。GI外科医生Jason Sicklick是 BRPT临床事务副主任。Sharmeela Kaushal是BRPT经理，负责监督 在NCI和CAP准则下的BRPT的操作方面。患者同意的三名协调员和 两名组织处理技术人员协助她。瓦莱里娅·埃斯特拉达，组织技术助理主任， 监督组织技术共享资源(TTSR)。她是一位训练有素的学术病理学家， 有管理和雇佣政策方面的经验，对标准/新颖的病理学有广泛的了解 技巧。她管理着四名敬业的组织技术人员。TTSR服务包括组织固定、加工 切片、石蜡切片、冰冻切片、常规染色、特殊染色、个体化免疫组织化学(IHC) 和免疫荧光(IF)分析、抗体优化和验证、数字成像和数字图像 储存，小鼠表型鉴定，组织微阵列开发和构建，多路IHC和IF，以及 病理咨询和培训等服务。自2016年7月以来，TTSR设备库存 在收购AT2 ScanScope幻灯片扫描仪、Gemini H&E等设备后，该公司实现了增长 Stainer和Biocare医学免疫组织化学自动染色。在过去的5年里，BTTSR已经 支持174名用户，包括89名MCC成员和11名来自索尔克和桑福德-伯纳姆的成员 Prebys NCI指定的癌症中心。

BIOREPOSITORY AND TISSUE TECHNOLOGY SHARED RESOURCE ABSTRACT The Biorepository and Tissue Technology Shared Resource (BTTSR) is a critical component of the infrastructure supporting translational studies. It is founded on a well-established institutional model integrating tissue procurement, processing, storage, and multimodal (histologic, immunohistochemical, and molecular) analyses with traditional epidemiologic, clinicopathologic and outcome parameters through a highly evolved bioinformatics system. Alfredo Molinolo, a physician pathologist with more than 40 years’ experience in human and experimental pathology, is the Director of the BTTSR. As of January 2018, the Biorepository (BRPT) is now a full College of American Pathologist (CAP)-accredited resource. Under its own IRB approved protocol (IRB# 090401), the BRPT integrates patient consenting, prospective procurement services, protocol and standard operating procedure design, tissue processing, and storage. Jason Sicklick, a GI surgeon, is the Deputy Director of the BRPT for clinical affairs. Sharmeela Kaushal is the BRPT Manager overseeing operational aspects of the BRPT under NCI and CAP guidelines. Three coordinators for patient consenting and two technicians for tissue processing assist her. Valeria Estrada, the Assistant Director for Tissue Technology, supervises the Tissue Technology Shared Resource (TTSR). She is a well-trained academic pathologist with experience in management and employment policies, and extensive knowledge of standard/novel pathology techniques. She supervises four dedicated histotechnicians. TTSR services include tissue fixation, processing and sectioning, paraffin and frozen, routine stains, special stains, individualized immunohistochemistry (IHC) and immunofluorescence (IF) assays, antibody optimization and validation, digital imaging and digital image storage, mouse phenotyping, tissue microarrays developing and construction, multiplexing IHC and IF, and pathology consultation and training, among other services. Since July 2016, the TTSR equipment inventory has grown with the acquisition of, among other equipment, an AT2 ScanScope slide scanner, a Gemini H&E stainer, and a Biocare Medical immunohistochemistry autostainer. Over the past 5 years, the BTTSR has supported 174 users, including 89 MCC members and 11 members from the Salk and Sanford-Burnham Prebys NCI-designated Cancer Centers.