权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Vanderbilt O'Brien Kidney Center-Core A Physiology-Pathiophysiology Core

范德比尔特奥布莱恩肾脏中心-核心生理学-病理生理学核心

基本信息

批准号：
10163164
负责人：
Mingzhi Zhang
金额：
$ 31.6万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-08 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10163164
关键词：
Acute Address Animals Bioenergetics CRISPR/Cas technology Clustered Regularly Interspaced Short Palindromic Repeats Communities Complement Drug Side Effects Education Ethylnitrosourea Experimental Models Functional disorder Gene Delivery Generations Genes Genetic Genome Imaging Techniques Immunotherapy Information Dissemination Infrastructure Injury Injury to Kidney International Intervention Kidney Kidney Diseases Kidney Function Tests Mass Spectrum Analysis Measurement Metabolic Metabolism Methodology Methods Modeling Mus Mutant Strains Mice Mutation Oxygen Oxygen Consumption Pathogenesis Pharmacologic Substance Phenotype Physiology Pilot Projects Renal function Reproducibility of Results Research Research Personnel Resources Services Structure TNFSF5 gene Testing Therapeutic Intervention Time Tissues Training Venous base blood flow measurement cost effective design design and construction drug testing glucose uptake in vivo insight interest investigator training kidney imaging mouse model mutant new technology non-invasive imaging novel overexpression prevent programs screening tool

项目摘要

ABSTRACT The Phenotyping and Pathophysiology Core is designed to address the need for effective phenotyping of kidney function in mouse models of selective gene overexpression or deletion. In addition, this Core is designed to provide experimental mouse models of acute or progressive renal injury to assess potential therapeutic interventions. This Core also helps investigators who may have developed genetic mouse models or potential therapeutic interventions but do not have the expertise and/or infrastructure required to design and carry out appropriate kidney phenotyping studies or studies investigating mouse models of kidney injury. In this proposal, we propose five distinct subcores that will offer and/or develop methodologies to define the pathogenesis of kidney disease and design therapeutic interventions. The Phenotyping Subcore will provide a battery of well-established invasive and non-invasive tests of kidney function. The Injury Model Subcore will provide investigators cost-effective testing of potential pharmaceutical or immunologic therapy strategies in appropriate mouse models of acute or progressive kidney injury. In addition, this subcore will provide murine models for screening of potential kidney side effects of drugs. This subcore provides a wide variety of experimental models of mouse kidney injury that offer consistent and reproducible results. Some of the mouse models available have been developed by the Center investigators. The Metabolism and Bioenergetics Subcore will offer direct measurements of kidney tissue partial oxygen consumption and will develop methodology for the measurements of whole kidney oxygen consumption based on kidney blood flow and measurements of arterial and renal venous partial oxygen. Furthermore this subcore will provide expertise in metabolic flux analysis, glucose uptake and quantification of various metabolites in kidney tissues using mass spectrometry methods. The Mouse Kidney Imaging Subcore will provide established non-invasive imaging techniques for the assessments of mouse renal diseases and will provide methods and further optimize novel methods for the noninvasive characterization of renal microstructure and function in mice. The CRISPR/cas9 Subcore will provide guidance for the design, construction, and generation of a CRISPR mouse. In summary, the Phenotyping and Pathophysiology Core provides a broad range of services that aid researchers interested in utilizing murine models for kidney-related research. The structure of this Core is unique in that it performs a variety of kidney functions, non-invasive quantitative kidney images analysis, and experimental platforms for the testing of drugs and interventions. Importantly, this Core performs all these services in house and directly oversees these studies. We have been very successful in providing these services not only to the local scientific community interested in kidney research, but also in serving as a national and international resource.

摘要