Enhancing Food Testing Capabilities at the WVDA through Discipline D: Special Projects Analytical Track 3: Method Development and Validation

2020-2021财年实验室灵活资助模式 西弗吉尼亚州农业部提案 PAR-20-105： 不允许进行LFFM(U19)临床试验 学科D：轨道3：特殊项目 摘要： 大多数类型的沙门氏菌都会引起一种叫做沙门氏菌病的疾病，这种疾病会导致 腹泻、发烧和胃痉挛。症状通常在6小时至6天后开始 感染，持续四到七天。然而，有些人不会出现症状。 感染后会持续几周，其他人会出现几周的症状。 沙门氏菌菌株有时会导致尿液、血液、骨骼、关节或 神经系统(脊髓液和脑)，并可导致严重疾病。沙门氏菌活着 在人和动物的肠道中。人们可以通过感染沙门氏菌 各种来源，包括食用受污染的食物或饮用受污染的水 以及接触受感染的动物、它们的粪便或它们的环境。疾控中心估计 沙门氏菌导致约135万人患病，26500人住院，420人死亡 在美国每年都有。 使用ABI 7500的定量聚合酶链式反应是获得实时检测结果的一种快速且经济实惠的方法 一株从WVDA微生物学正常食品检测方法中恢复的分离物 实验室。在韦斯特的微生物实验室中加入这一水平的技术 弗吉尼亚州农业部将允许该部门在 实时设置并协助更快地进行疫情检测。WVDA目前有 拥有ABI 7500 FAST，并准备加入多实验室验证利用 这项技术用于沙门氏菌的分离。 规程D的目的：轨道3，方法开发和方法验证 第1期是与本跟踪中的其他实验室一起参与多实验室验证 利用定量聚合酶链式反应技术进一步研究西方沙门氏菌分离株 弗吉尼亚州农业部监管和环境事务部。 建设这一能力将包括购买配套的工具包、实验室 补给和消耗品。包括在多实验室验证中的内容将继续 提高WVDA微生物学实验室的能力和能力。 在实验室灵活筹资模式的第二至第五阶段，WVDA将 致力于FDA在规程5：轨道3-方法中指定的项目 开发和方法验证。WVDA在方法方面有丰富的经验 开发和验证，并将是第二阶段到第二阶段的优秀候选人 LFFM的这条轨道的5。

Laboratory Flexible Funding Model FY2020-2021 West Virginia Department of Agriculture Proposal PAR-20-105: LFFM (U19) Clinical Trials Not Allowed Discipline D: Track 3: Special Projects Abstract: Most types of Salmonella cause an illness called salmonellosis, which can cause diarrhea, fever, and stomach cramps. Symptoms usually begin six hours to six days after infection and last four to seven days. However, some people do not develop symptoms for several weeks after infection and others experience symptoms for several weeks. Salmonella strains sometimes cause infection in urine, blood, bones, joints, or the nervous system (spinal fluid and brain) and can cause severe disease. Salmonella lives in the intestines of people and animals. People can acquire Salmonella infection from a variety of sources, including eating contaminated food or drinking contaminated water and touching infected animals, their feces, or their environment. CDC estimates Salmonella cause about 1.35 million illnesses, 26,500 hospitalizations, and 420 deaths in the United States every year. qPCR using the ABI 7500 is a fast and affordable way to obtain real-time test results for an isolate recovered from the normal food testing protocol of the WVDA microbiology laboratory. Adding this level of technology within the Microbiology laboratory at West Virginia Department of Agriculture will allow the department to obtain bacterial data in a real-time setting and assist in faster outbreak detection. The WVDA currently has possession of the ABI 7500 fast and are prepared to join in a multi-lab validation utilizing this technology for Salmonella isolates. The purpose of Discipline D: Track 3, Method Development and Method Validation for period 1 is to participate with other laboratories within this track in a multi-lab validation utilizing a qPCR instrument to further studies of Salmonella isolates within the West Virginia Department of Agriculture Regulatory and Environmental Affairs Division. Building this capacity will include the purchase of accompanying kits, laboratory supplies, and consumables. The inclusion in this multi-lab validation will continue to increase the capabilities and capacity of the microbiology laboratory of the WVDA. In periods two through five of the Laboratory Flexible Funding Model, the WVDA will be committed to FDA assigned projects given in Discipline 5: Track 3 – Method Development and Method Validation. WVDA has extensive experience with method developments and validations and would be an excellent candidate for periods 2 through 5 of this track of the LFFM.