Action and interaction of ionotropic and metabotropic neurotransmission

离子型和代谢型神经传递的作用和相互作用

• 批准号: 10166240
• 负责人: Bernardo L Sabatini
• 金额: $ 2.35万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10166240
• 关键词: Acetylcholine; American; Bachelor' s Degree; Biological Assay; Biology; Biomedical Research; Brain; Cameroon; Cyclic AMP-Dependent Protein Kinases; Development; Development Plans; Doctor of Philosophy; Educational workshop; Enrollment; Ethnic group; Goals; Image; Measures; Mediator of activation protein; Mentors; Mentorship; Methods; Microglia; Morphology; NCI Scholars Program; Neuroglia; Neurons; Neurosciences; Neurosciences Research; Neurotransmitters; Postdoctoral Fellow; Preparation; Research; Schools; Signal Transduction; Signaling Molecule; Slice; Testing; Training; Underrepresented Groups; Universities; Woman; career; career development; design; falls; gamma-Aminobutyric Acid; laboratory experience; meetings; neurotransmission; parent grant; programs; receptor; research and development; response; symposium; tutoring

Abstract This is an application for an administrative diversity supplement to R37 NS046579-16, “Action and interaction of ionotropic and metabotropic neurotransmission” to support the scientific training and development of Rochinelle Dongmo. Ms. Dongmo is a baccalaureate degree holder from Emory University who is currently enrolled in a post-baccalaureate research scholars program at Harvard to prepare for Ph.D. graduate programs in neuroscience, with the goal of enrolling in a program by the Fall of 2021. As a black woman who is a U.S. permanent resident currently finishing her naturalization as an American citizen from Cameroon, she is part of an ethnic group underrepresented in biomedical research. Ms. Dongmo's research in the lab will pursue two aims related to the parent grant, both of them seeking to better understand the mechanism of metabotropic neurotransmission to non-neuronal microglia. In the first aim, Ms. Dongmo will develop an imaging assay to assess microglia activation in ex vivo brain slices via changes in gross morphology. This assay will allow us to test the activation of microglia to multiple neurotransmitters, most notably acetylcholine and GABA, for which microglia are known to possess metabotropic receptors. In the second aim, Ms. Dongmo will measure the effects of microglia activation on the intracellular activity of Ca2+ and protein kinase A (PKA), two central intracellular signaling molecules. She will test the Ca2+ and PKA activity in response to both known activators of microglia, as well as to GABA and acetylcholine, whose net effect on microglia activation will be determined in her first aim. She will be mentored during the execution of this research plan on both all scientific and technical aspects of the research. We have designed a comprehensive mentorship and training plan for Ms. Dongmo including lab experience, regular one-on-one meetings with the PI and a senior post-doc, conference presentations, all in addition to her participation in the Harvard Research Scholars Initiative, which includes classes, workshops, tutoring, GRE test preparation, and graduate school application guidance. These research and career development plans will prepare Ms. Dongmo for a successful academic career in research neuroscience.

摘要 这是对R37 NS 046579 -16“行动和互动”的行政多样性补充申请 离子型和代谢型神经传递”，以支持Rochinelle的科学训练和发展 东莫。Dongmo女士是埃默里大学的学士学位保持器持有者，目前就读于 哈佛的学士后研究学者项目，为博士学位做准备。研究生课程 神经科学，目标是在2021年秋季之前报名参加一个项目。作为一个黑人妇女，她是美国的一员。 她是一名永久居民，目前正在完成从喀麦隆入籍为美国公民的手续， 一个在生物医学研究中代表性不足的种族群体。 女士Dongmo在实验室的研究将追求两个与父母补助金相关的目标，这两个目标都是为了更好地 了解代谢型神经传递到非神经元小胶质细胞的机制。在第一个目标，女士。 Dongmo将开发一种成像检测方法，通过总的变化来评估离体脑切片中小胶质细胞的激活。 形态学这项试验将使我们能够测试小胶质细胞对多种神经递质的激活，最值得注意的是， 乙酰胆碱和GABA，已知小胶质细胞具有其代谢型受体。第二个目标， 女士Dongmo将测量小胶质细胞活化对细胞内Ca 2+和蛋白激酶活性的影响 蛋白激酶A（PKA），两种中心细胞内信号分子。她将检测钙离子和PKA活性， 这两种已知的小胶质细胞激活剂，以及GABA和乙酰胆碱，其对小胶质细胞激活的净效应 这将取决于她的第一个目标。她将在执行本研究计划期间接受指导， 研究的科学和技术方面。我们设计了全面的指导和培训 Dongmo女士的计划，包括实验室经验，与PI和高级博士后的定期一对一会议， 除了参加哈佛研究学者倡议外， 包括课程、研讨会、辅导、GRE考试准备和研究生院申请指导。这些 研究和职业发展计划将为Dongmo女士在研究方面的成功学术生涯做好准备 神经科学