Peptide Modulation of Striatal Patch and Matrix Subdivisions

纹状体斑块和基质细分的肽调节

• 批准号: 9125895
• 负责人: Bernardo L Sabatini
• 金额: $ 41.75万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9125895
• 关键词: Acute; Axon; Basal Ganglia; Behavior; Biochemical; Biology; Brain; Cell Nucleus; Cells; Cholecystokinin; Corpus striatum structure; Coupled; Cyclic AMP-Dependent Protein Kinases; Development; Diffuse; Disease; Dopamine; Dorsal; Drug Addiction; Dynorphins; Electrophysiology (science); Enkephalins; Equilibrium; Genetic; Glutamates; Goals; Health; Heart; Human; Huntington Disease; In Vitro; Individual; Laboratories; Light; Neuromodulator; Neurons; Neuropeptides; Neurophysiology - biologic function; Neurotransmitters; Opioid; Opioid Receptor; Optics; Parkinson Disease; Pathway interactions; Pattern; Peptide Receptor; Peptide Signal Sequences; Peptides; Pharmaceutical Preparations; Physiologic pulse; Play; Process; Property; Proteins; Reagent; Receptor Activation; Regulation; Research; Rodent; Role; Route; Signal Pathway; Signal Transduction; Site; Slice; Somatostatin; Staging; Structure; Substance P; Synapses; Synaptic Transmission; System; Therapeutic; Tissues; Transgenic Mice; Work; analog; base; brain cell; brain tissue; cellular targeting; gamma-Aminobutyric Acid; human disease; improved; in vivo; insight; motor control; neuropeptide Y; neuropsychiatric disorder; neuropsychiatric symptom; neurotransmission; neurotransmitter release; new technology; novel; optogenetics; receptor coupling; receptor expression; striosome; synaptic function; tool; two-photon

DESCRIPTION (provided by applicant): The mammalian brain, including the human brain, contains dozens of peptides that act as neurotransmitters between cells but whose function and modes of action are relatively unknown. This is particularly true in the basal ganglia in which the expression of peptides and of peptide receptors varies from cell to cell and nucleus to nucleus. The basal ganglia play a central role in controlling motor action selection - i.e. deciding what we do - and are at the heart of human disorders such as Parkinson's, Huntington's and drug addiction. We propose to study the function of peptides in the basal ganglia in order to understand how they signal and control the circuitry of this structure. We will make use of new technology developed in the laboratory that allows us to precisely release peptides in mammalian brain tissue using pulses of light. This will be used to analyze peptide function in tissue in which specific classes of neurons are marked or placed under optogenetic control. The proposed work will shed light on the basic biology of this conserved signaling system and will inform how its perturbation contributes to disease.

描述（由申请人提供）：哺乳动物的大脑，包括人类的大脑，包含几十种多肽，它们作为细胞之间的神经递质，但其功能和作用方式相对未知。在基底神经节中尤其如此