OHSU Neuroscience Imaging Center

项目摘要 俄勒冈州健康与科学大学（OHSU）神经科学成像中心将提供以下专业知识： 设计，进行和分析光和电子的最先进仪器的实验 显微镜神经科学成像中心有两个科学核心：核心1-分析荧光 成像和核心2-超微结构和单粒子显微镜。这些核心的仪器在 在过去的六年里，地方和技术能力得到了极大的扩展， 只有共聚焦显微镜和透射电子显微镜，还有体内双光子显微镜， 超分辨率显微镜、EM断层扫描和单粒子分析， 结构.所有的仪器都位于大学范围内的服务中心，由办公室的支持， 研究副总裁。神经科学成像中心为NINDS资助的OHSU研究人员提供服务， 以及其他NIH资助的从事神经科学研究的研究人员。目前有21个NINDS- 资助的研究人员谁提交了合格的项目，目前的建议，并有显微镜和 他们目前的研究需要成像。其他OHSU神经科学家也得到核心工作人员的支持。 共享工具的用户费用部分由P30赠款支持，NINDS的支持大于 非NINDS调查员;指导委员会定期审查使用费支助。显微镜岩心 设施位于Marquam Hill校区和邻近的Waterfront校区（请参阅参考资料）， 所有P30支持的调查人员都将开放访问所有这些资源。OHSU神经科学 成像中心赠款为核心员工提供工资支持，他们是本地的，面向服务的技术 专家神经科学成像中心主任Sue Aicher博士和副主任Catherine博士 摩根拥有广泛的科学和技术专长，将提供前端咨询， 研究人员，帮助指导每个研究人员适当的资源，以解决他们的研究目标。 指导委员会向中心主任提供核心战略和业务方面的投入。 问题，以及项目优先级和核心访问计划。我们提供不同级别的支持， 调查人员的需要。具有广泛成像专业知识的调查人员主要需要访问状态- 最先进的设备和偶尔与工作人员协商。其他调查人员需要更深入的支持， 并经常为电子显微镜研究提供全面的服务支持。P30工作人员将提供专家 提供适当的支持，以满足我们参与研究者的需求。神经科学 成像中心促进了NINDS研究人员和其他神经科学家之间的互动， OHSU为先进显微镜的投资提供了协同作用，并显着提高了科学研究的效率。 神经科学界的进步

Project Summary The Oregon Health & Science University (OHSU) Neuroscience Imaging Center will provide expertise in designing, conducting, and analyzing experiments on state-of-the-art instrumentation for light and electron microscopy. The Neuroscience Imaging Center has two scientific Cores: Core 1 - Analytical Fluorescence Imaging and Core 2 - Ultrastructure and Single Particle Microscopy. The instruments for these Cores are in place and the technical capacities have been expanded tremendously over the past six years to include not only confocal microscopy and transmission electron microscopy, but also in vivo two-photon microscopy, super-resolution microscopy, EM tomography and single particle analyses for determining molecular structures. All instruments are located in university-wide service centers that are supported by the office of the Vice President for Research. The Neuroscience Imaging Center serves NINDS-funded OHSU researchers, as well as other NIH-funded investigators engaged in neuroscience research. We currently have 21 NINDS- funded investigators who have submitted qualifying projects for the current proposal and have microscopy and imaging needs for their current research. Other OHSU neuroscientists also receive support from Core staff. User fees on shared instruments are partially supported by the P30 grant, with greater support for NINDS than non-NINDS investigators; user fee support is regularly reviewed by the Steering Committee. Microscopy core facilities are located on the Marquam Hill campus and at the adjacent Waterfront Campus (see Resources), and all P30-supported investigators will have open access to all of these resources. The OHSU Neuroscience Imaging Center grant provides salary support for Core staff, who are local, service-oriented technology experts. The Neuroscience Imaging Center Director Dr. Sue Aicher and Associate Director Dr. Catherine Morgans have extensive scientific and technical expertise and will provide front-end consultation with investigators, helping to direct each investigator to the appropriate resources to address their research goals. The Steering Committee provides input to the Center Director with regard to Core strategic and operational issues, as well as project prioritization and Core access plans. We provide different levels of support based on the needs of the investigators. Investigators with extensive imaging expertise primarily require access to state- of-the-art equipment and occasional consultation with staff. Other investigators need more in-depth support, and full service support is often provided for electron microscopy studies. The P30 staff will provide expert support at the appropriate level to meet the needs of our participating investigators. The Neuroscience Imaging Center has catalyzed interactions among NINDS researchers as well as other neuroscientist at OHSU, provided synergy for investments in advanced microscopy, and significantly enhanced scientific progress for our neuroscience community.