OHSU Neuroscience Imaging Center

项目摘要 俄勒冈健康与科学大学(OHSU)神经科学成像中心将提供 设计、进行和分析最先进的光与电子仪器实验 显微镜。神经科学成像中心有两个科学核心：核心1-分析荧光 成像和岩芯2-超微结构和单粒子显微镜。这些岩心的仪器都在 Place和技术能力在过去六年中得到了极大的扩展，包括 不仅有共焦显微镜和透射电子显微镜，还有活体双光子显微镜， 用于确定分子的超分辨显微镜、EM层析和单粒子分析 结构。所有仪器都位于大学范围内的服务中心，这些服务中心由 负责研究的副总裁。神经科学成像中心为NINDS资助的OHSU研究人员提供服务，如 以及其他由美国国立卫生研究院资助的从事神经科学研究的研究人员。我们目前有21个NIND- 资助调查人员，他们提交了符合当前提案条件的项目，并拥有显微镜和 他们目前研究的成像需求。OHSU的其他神经科学家也得到了核心工作人员的支持。 共享仪器的使用费部分由P30拨款支持，对NINDS的支持比 非NINDS调查员；指导委员会定期审查用户费用支持。显微岩心 设施位于Marquam Hill校区和邻近的滨水校区(见参考资料)， 所有P30支持的调查人员都将可以开放访问所有这些资源。OHSU神经科学 影像中心补助金为核心员工提供工资支持，这些员工是本地的、面向服务的技术人员 专家。神经科学成像中心主任苏·艾歇尔博士和副主任凯瑟琳博士 摩根公司拥有广泛的科学和技术专长，将为 研究人员，帮助指导每个研究人员找到适当的资源，以解决他们的研究目标。 指导委员会就核心战略和业务向中心主任提供意见 问题，以及项目优先顺序和核心接入计划。我们基于以下方面提供不同级别的支持 调查人员的需求。拥有广泛成像专业知识的调查人员主要需要访问国家- 最先进的设备和偶尔与员工进行咨询。其他调查人员需要更深入的支持， 并经常为电子显微镜研究提供全方位的服务支持。P30的工作人员将提供专家 提供适当级别的支持，以满足我们参与调查人员的需要。神经科学 影像中心催化了NINDS研究人员以及其他神经科学家之间的互动， OHSU，为先进显微镜的投资提供了协同效应，并显著增强了科学 为我们的神经科学界取得进展。

Project Summary The Oregon Health & Science University (OHSU) Neuroscience Imaging Center will provide expertise in designing, conducting, and analyzing experiments on state-of-the-art instrumentation for light and electron microscopy. The Neuroscience Imaging Center has two scientific Cores: Core 1 - Analytical Fluorescence Imaging and Core 2 - Ultrastructure and Single Particle Microscopy. The instruments for these Cores are in place and the technical capacities have been expanded tremendously over the past six years to include not only confocal microscopy and transmission electron microscopy, but also in vivo two-photon microscopy, super-resolution microscopy, EM tomography and single particle analyses for determining molecular structures. All instruments are located in university-wide service centers that are supported by the office of the Vice President for Research. The Neuroscience Imaging Center serves NINDS-funded OHSU researchers, as well as other NIH-funded investigators engaged in neuroscience research. We currently have 21 NINDS- funded investigators who have submitted qualifying projects for the current proposal and have microscopy and imaging needs for their current research. Other OHSU neuroscientists also receive support from Core staff. User fees on shared instruments are partially supported by the P30 grant, with greater support for NINDS than non-NINDS investigators; user fee support is regularly reviewed by the Steering Committee. Microscopy core facilities are located on the Marquam Hill campus and at the adjacent Waterfront Campus (see Resources), and all P30-supported investigators will have open access to all of these resources. The OHSU Neuroscience Imaging Center grant provides salary support for Core staff, who are local, service-oriented technology experts. The Neuroscience Imaging Center Director Dr. Sue Aicher and Associate Director Dr. Catherine Morgans have extensive scientific and technical expertise and will provide front-end consultation with investigators, helping to direct each investigator to the appropriate resources to address their research goals. The Steering Committee provides input to the Center Director with regard to Core strategic and operational issues, as well as project prioritization and Core access plans. We provide different levels of support based on the needs of the investigators. Investigators with extensive imaging expertise primarily require access to state- of-the-art equipment and occasional consultation with staff. Other investigators need more in-depth support, and full service support is often provided for electron microscopy studies. The P30 staff will provide expert support at the appropriate level to meet the needs of our participating investigators. The Neuroscience Imaging Center has catalyzed interactions among NINDS researchers as well as other neuroscientist at OHSU, provided synergy for investments in advanced microscopy, and significantly enhanced scientific progress for our neuroscience community.