OHSU Neuroscience Imaging Center

OHSU 神经科学影像中心

• 批准号: 10169583
• 负责人: SUE A AICHER
• 金额: $ 15.33万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10169583
• 关键词: 3-Dimensional; Address; Basic Science; Biological Sciences; Brain; Cancer Grant Supplements (P30); Communities; Confocal Microscopy; Consultations; Core Facility; Coupled; Cryoelectron Microscopy; Electron Microscope; Electron Microscopy; Equipment; Face; Fees; Funding; Goals; Grant; Health Sciences; Image; Imaging technology; Institutes; Investments; Light; Manuscripts; Methods; Microscope; Microscopy; Mission; Molecular Structure; National Institute of Neurological Disorders and Stroke; Neurosciences; Neurosciences Research; Optics; Oregon; Pathologic; Primates; Public Health; Publications; Publishing; Qualifying; Research; Research Personnel; Resolution; Resources; Scientist; Services; Technical Expertise; Technology; Time; Training; Transmission Electron Microscopy; United States National Institutes of Health; Universities; University Hospitals; Wages; base; cost; design; digital; experience; experimental study; falls; federal policy; fluorescence imaging; imaging approach; imaging study; immunocytochemistry; in vivo; instrument; instrumentation; light microscopy; microscopic imaging; nervous system disorder; particle; ranpirnase; reconstruction; synergism; technology development; tomography; two photon microscopy

Project Summary The Oregon Health & Science University (OHSU) Neuroscience Imaging Center will provide expertise in designing, conducting, and analyzing experiments on state-of-the-art instrumentation for light and electron microscopy. The Neuroscience Imaging Center has two scientific Cores: Core 1 - Analytical Fluorescence Imaging and Core 2 - Ultrastructure and Single Particle Microscopy. The instruments for these Cores are in place and the technical capacities have been expanded tremendously over the past six years to include not only confocal microscopy and transmission electron microscopy, but also in vivo two-photon microscopy, super-resolution microscopy, EM tomography and single particle analyses for determining molecular structures. All instruments are located in university-wide service centers that are supported by the office of the Vice President for Research. The Neuroscience Imaging Center serves NINDS-funded OHSU researchers, as well as other NIH-funded investigators engaged in neuroscience research. We currently have 21 NINDS- funded investigators who have submitted qualifying projects for the current proposal and have microscopy and imaging needs for their current research. Other OHSU neuroscientists also receive support from Core staff. User fees on shared instruments are partially supported by the P30 grant, with greater support for NINDS than non-NINDS investigators; user fee support is regularly reviewed by the Steering Committee. Microscopy core facilities are located on the Marquam Hill campus and at the adjacent Waterfront Campus (see Resources), and all P30-supported investigators will have open access to all of these resources. The OHSU Neuroscience Imaging Center grant provides salary support for Core staff, who are local, service-oriented technology experts. The Neuroscience Imaging Center Director Dr. Sue Aicher and Associate Director Dr. Catherine Morgans have extensive scientific and technical expertise and will provide front-end consultation with investigators, helping to direct each investigator to the appropriate resources to address their research goals. The Steering Committee provides input to the Center Director with regard to Core strategic and operational issues, as well as project prioritization and Core access plans. We provide different levels of support based on the needs of the investigators. Investigators with extensive imaging expertise primarily require access to state- of-the-art equipment and occasional consultation with staff. Other investigators need more in-depth support, and full service support is often provided for electron microscopy studies. The P30 staff will provide expert support at the appropriate level to meet the needs of our participating investigators. The Neuroscience Imaging Center has catalyzed interactions among NINDS researchers as well as other neuroscientist at OHSU, provided synergy for investments in advanced microscopy, and significantly enhanced scientific progress for our neuroscience community.

项目摘要 俄勒冈健康与科学大学（OHSU）神经科学成像中心将提供专业知识 设计，进行和分析光和电子最新仪器的实验 显微镜。神经科学成像中心有两个科学核心：核心1-分析荧光 成像和核心2-超微结构和单个粒子显微镜。这些核心的仪器在 在过去的六年中，地点和技术能力大大扩展，不包括 仅共聚焦显微镜和透射电子显微镜，但还具有体内两光子显微镜， 超分辨率显微镜，EM层析成像和单个粒子分析，以确定分子 结构。所有工具均位于大学范围内的服务中心，并由该办公室支持 研究副总裁。神经科学成像中心为Ninds资助的OHSU研究人员提供服务，作为 以及其他从事神经科学研究的研究人员。我们目前有21个Ninds- 资助的调查人员已提交了当前建议的合格项目，并具有显微镜和 他们当前研究的成像需求。其他OHSU神经科学家也得到核心人员的支持。 共享工具的用户费用得到了P30赠款的部分支持，对NIND的支持比 非核心调查员；指导委员会定期审查用户费用支持。显微镜核心 设施位于Marquam Hill校园和邻近的海滨校园（见资源）， 所有由P30支持的调查人员都将公开访问所有这些资源。 OHSU神经科学 Imaging Center Grant为核心人员提供薪水支持，这些人员是当地的，面向服务的技术 专家。神经科学成像中心主任Sue Aicher博士和副主任Catherine博士 Morgans具有广泛的科学和技术专业知识，并将与 调查人员，帮助将每个调查员引导到适当的资源以解决其研究目标。 指导委员会就核心战略和运营提供了中心主任的意见 问题，项目优先级和核心访问计划。我们基于 调查人员的需求。具有广泛成像专业知识的研究人员主要需要进入州 - 设备和偶尔与员工进行咨询。其他调查人员需要更多的深入支持， 通常为电子显微镜研究提供全面的服务支持。 P30员工将为专家提供 在适当水平上的支持，以满足我们参与的调查人员的需求。神经科学 成像中心已经催化了NINDS研究人员以及其他神经科学家之间的相互作用 OHSU，为高级显微镜投资提供了协同作用，并显着增强了科学 我们的神经科学社区的进步。

项目成果

Prenatal cerebral ischemia disrupts MRI-defined cortical microstructure through disturbances in neuronal arborization.

• DOI: 10.1126/scitranslmed.3004669
• 发表时间: 2013-01-16
• 期刊: Science translational medicine
• 影响因子: 17.1
• 作者: Dean JM;McClendon E;Hansen K;Azimi-Zonooz A;Chen K;Riddle A;Gong X;Sharifnia E;Hagen M;Ahmad T;Leigland LA;Hohimer AR;Kroenke CD;Back SA
• 通讯作者: Back SA

The effect of long-term ovariectomy on midbrain stress systems in free ranging macaques.

• DOI: 10.1016/j.brainres.2012.09.035
• 发表时间: 2012-12-07
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Bethea CL;Reddy AP
• 通讯作者: Reddy AP

Neuropeptide Y suppresses thermogenic and cardiovascular sympathetic nerve activity via Y1 receptors in the paraventricular nucleus and dorsomedial hypothalamus.

• DOI: 10.1111/jne.13006
• 发表时间: 2021-08
• 期刊: Journal of neuroendocrinology
• 影响因子: 3.2
• 作者: Shi Z;Bonillas AC;Wong J;Padilla SL;Brooks VL
• 通讯作者: Brooks VL

Quality control protocol for confocal systems.

共焦系统的质量控制协议。

• DOI: 10.1016/b978-0-12-407239-8.00003-3
• 发表时间: 2013
• 期刊: Methods in cell biology
• 作者: Cornea,Anda

Orientation selectivity in rabbit retinal ganglion cells is mediated by presynaptic inhibition.

• DOI: 10.1523/jneurosci.2081-10.2010
• 发表时间: 2010-11-17
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Venkataramani S;Taylor WR
• 通讯作者: Taylor WR