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Imaging Research Core

影像研究核心

基本信息

批准号：
10165747
负责人：
Gregg W Roman
金额：
$ 19.88万
依托单位：
UNIVERSITY OF MISSISSIPPI
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-05-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10165747
关键词：
Address Awareness Biological Biological Assay Carbohydrates Cell physiology Cells Cellular Structures Cellular biology Centers of Research Excellence Chemical Structure Complex Computer Assisted Computer Workstations Computer software Computers and Advanced Instrumentation Confocal Microscopy Consult Data Development Disease Ensure Extracellular Structure Fiji Fluorescence Resonance Energy Transfer Goals Health Human Image Image Analysis Imaging Techniques Individual Instruction Label Laser Scanning Confocal Microscopy Lectin Location Maintenance Measures Methods Microscope Microscopic Microscopy Mississippi Modernization Molecular Organism Phase-Contrast Microscopy Physiological Polysaccharides Preparation Property Protocols documentation Reagent Research Research Personnel Resolution Role Sampling Schedule Specific qualifier value Specificity Structural Chemistry Subcellular structure System Techniques Time Tissues Training Training Programs Universities automated analysis imager imaging platform imaging system in vivo insight instrument instrumentation molecular dynamics novel strategies operation particle physical property preservation programs protein protein interaction protein transport response tool

项目摘要

There is an unequivocal need to develop and employ advanced techniques in microscopy toward understanding the cellular functions of glycans. Glycans participate in many cellular properties, including cell structure, cell-cell recognition, protein trafficking, and protein-protein interaction. Hence, changes in glycan abundance or composition can have considerable effects on cellular function, and as a consequence human health. Advanced imaging techniques are becoming a vital part of an integrative approach to understanding the roles of glycans in health and disease. These techniques provide insights into molecular composition, while preserving the spatial context of the chemical, structural, and physiological responses to changes in glycans. Recent advances in identify lectin specificities and the availability of a broad range of fluorescently labeled and biotinylated lectins has rapidly advanced our ability to identify changes in the location and abundance of specific glycans in cells and tissues. Moreover, several powerful approaches in confocal microscopy, including FRET, FRAP, and FLIP, permit highly sensitive measures of molecular dynamics within living cells. These measures will help researchers understand the fundamental effect glycans have on cellular function. The central goal of this proposal is to bring the ability to employ advanced imaging techniques to the GlyCORE investigators in their diverse research programs and to help develop new approaches in the cell biology of glycans. These goals will be accomplished in three aims: 1) establish an imaging core that contains three different imaging platforms, including a powerful new confocal microscope; 2) support the GlyCORE investigators in their individual projects by working to develop new imaging applications; and 3) develop an extensive training program to develop expertise and awareness of new methods in imaging and image analysis. This proposal will transform research in the glycosciences at the University of Mississippi by providing access to advanced instrumentation and expertise and training in the use of these imaging systems.

有一个明确的需要，以发展和采用先进的技术，在显微镜