Single Cell Analysis Core (Core C)

单细胞分析核心（核心 C）

• 批准号: 10170969
• 负责人: Minsu Kim
• 金额: $ 37.1万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-05 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10170969
• 关键词: Antibiotics; Area; Bacteria; Bioreactors; Cell Maintenance; Cells; Clinical; Complement; Controlled Environment; Copy Number Polymorphism; Devices; Ensure; Environment; Fiber; Fluorescence; Fluorescent Probes; Fluorescent in Situ Hybridization; Gene Amplification; Gene Dosage; Gene Expression Profiling; Genotype; Glutathione Metabolism Pathway; Goals; Heterogeneity; Image; Image Analysis; Individual; Interdisciplinary Study; Intermediate resistance; Laboratories; Length; Measures; Metabolic; Metabolism; Methods; Microfluidics; Monitor; Nutrient; Physics; Physiology; Population; Prevalence; Process; Reporter; Research; Resolution; Scientist; Statistical Data Interpretation; Time; Universities; Variant; Waste Products; Work; experimental study; fluorescence microscope; glucose metabolism; interest; resistance gene; single cell analysis; single molecule

PROJECT SUMMARY/ABSTRACT - CORE C: Single-Cell Analysis Core HR arises from cell-to-cell variation of genotypic and metabolic processes. The goal of the Single-Cell Analysis Core (Core C) is to provide experimentally-validated and yet difficult-to-perform single-cell-level analyses to evaluate the mechanisms and dynamics of HR. Core C will support the single-cell-level studies proposed in all three Projects by providing a centralized platform for culturing bacterial cells under well-controlled antibiotic environments, measuring relevant processes at single-cell resolution, and performing image analysis as well as necessary statistical analysis. Specifically, Core C will i) provide microfluidic continuous culture platform, ii) quantify the copy number variation of resistance genes, and iii) determine metabolic activities of single cells in heteroresistant populations. Core C activities will be conducted by Dr. Minsu Kim's laboratory in the Department of Physics at Emory University, which has extensive expertise in single-cell-level imaging and analysis of gene expression, metabolism, and physiology in bacteria. Dr. Kim was one of the first scientists to apply a single-cell approach to antibiotic research and is considered a leading expert in this area. Dr. Kim and his team will work with the PI of individual projects collaboratively to ensure they can successfully complete their projects in a timely manner.

项目摘要/摘要-核心C：单细胞分析核心 HR由基因型和代谢过程的细胞间变异引起。单细胞分析的目标 核心（核心C）是提供实验验证，但难以执行的单细胞水平的分析， 评估HR的机制和动力学。核心C将支持所有研究中提出的单细胞水平的研究。 通过提供一个集中的平台，在良好控制的抗生素下培养细菌细胞， 环境，以单细胞分辨率测量相关过程，并执行图像分析，以及 必要的统计分析。具体地，核心C将i）提供微流体连续培养平台，ii） 定量抗性基因的拷贝数变异，和iii）测定单细胞在 异源抗性种群核心C活动将由Minsu Kim博士在该部门的实验室进行 埃默里大学的物理学教授，在单细胞水平的成像和基因分析方面拥有丰富的专业知识。 表达、代谢和生理学。金博士是最早将单细胞 他致力于抗生素研究，被认为是该领域的领先专家。金博士和他的团队 与各个项目的PI合作，以确保他们能够及时成功完成项目 方式