Point-scanning confocal with area detector

该仪器将用作研究显微镜，用于研究活细胞/固定细胞/组织的细胞过程。超分辨率探测器将允许超过理论分辨率限制，低至90纳米左右，并将提供出色的信噪比和高灵敏度。有了这项技术，我们可以在短时间和低光强度下解决甚至最小的细胞过程，这对活细胞成像特别重要。对于我们的相关光学和电子显微镜，特别是高分辨率的快速扫描时间对于在合理的时间内进行大体积扫描以及在支持与EM图像相关的分辨率下进行扫描非常重要。为了充分支持活细胞成像，环境室是可用的。该仪器将放置在显微镜核心设施中，作为仪器服务的中心场所。

The instrument is going to be used as a research microscope to study cellular processes of live/fixed cells/tissue. The super-resolution detector will allow for exceeding the theoretical resolution limit, down to around 90 nm, and it will provide excellent signal-to-noise ratio and high sensitivity. With this technology we can resolve even smallest cell processes at short times and with low light intensities, which is particular important for live cell imaging. For our correlative light and electron microscopy especially the fast scan times at high resolution are important to allow scans of large volume in a reasonable time but also at a resolution that supports correlation with the EM images. For full support of live cell imaging an environmental chamber is available. The instrument will be placed in a microscopy core facility to serve as a central place for instrument-based services.