Manufacture and Assembly of Thermoplastic, Modular, Integrated Fluidic Systems
热塑性、模块化、集成流体系统的制造和组装
基本信息
- 批准号:10172703
- 负责人:
- 金额:$ 19.07万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-16 至 2026-06-30
- 项目状态:未结题
- 来源:
- 关键词:AdoptedBacterial InfectionsBiologicalBiological AssayBiological MarkersBiopsy SpecimenBiotechnologyBloodBlood capillariesBlood specimenCell ExtractsCellsChIP-seqClinicalComplexDNADevelopmentDiagnosticDisease ManagementDrug resistanceElastomersElementsEnsureExonucleaseFailureFilmGenesGenomeGeometryGoalsHealthHeatingInjectionsLengthLiquid substanceMalignant NeoplasmsMembraneMetalsMicrofluidic MicrochipsMicrofluidicsMoldsMolecularMolecular AnalysisMutationNeoplasm Circulating CellsPartner in relationshipPatientsPlasmaPlayPolymersPositioning AttributePreparationProceduresProcessProductionProteinsRNAReactionReagentReportingResearch PersonnelResourcesSamplingSideStandardizationStressStrokeStructureSurfaceSystemSystems IntegrationTechnologyTimebaseclinical applicationcostdesignelastomericexperiencehigh standardimprovedinnovationinstrumentknowledge baselarge scale productionliquid biopsymanufacturing processnanofluidicnanosensorsnoveloperationprecision medicinepressurerapid techniqueresearch clinical testingsealsingle moleculesuccesstoolvirtual
项目摘要
TITLE: Biotechnology Resource Center of BioModular Multi-scale Systems (CBM2) for Precision Medicine
TR&D 3: Manufacture and Assembly of Thermoplastic, Modular, Integrated Fluidic Systems
Abstract/Summary
One of the primary limitations in the application of liquid biopsy markers to a diverse set of clinical problems,
such as cancer, stroke, and drug-resistant bacterial infections, is the mass-limits they impose on the
associated molecular assays. Mass limits are particularly problematic when using benchtop instruments and
the associated sample handling. Most liquid biopsy-based molecular assays require multiple steps, each
with a complex workflow, and the added problem of transferring small quantities of targets, such as DNA,
RNA, or proteins, from one instrument to the next. Mixed-scale fluidic systems offer the potential to compress
the entire set of assays into a single, integrated platform reducing sample loss, operator expertise, and
simpler workflows. The goal of CBM2 is to develop technologies to make modular, integrated systems available
for routine clinical use, even for mass-limited samples. Technologies evolving from this project will reduce
the complexity of building and operating integrated and modular fluidic systems specifically designed to
analyze liquid biopsy markers.
In order to reduce the complexity of building such systems, the platforms should be conducive to high-scale
production, which points to the use of injection molded thermoplastics. The modular concept facilitates this
by using the same or similar modules for different assays, ability to use the modules as standalone units,
and a motherboard containing standardized interconnects to host the modules. The focus of TR&D 3 is to
develop technologies for manufacturing and assembly of injection molded motherboards and modules.
High thermal expansion metals will enable robust mixed-scale mold inserts and reduce demolding stresses. A
novel, scalable, repeatable and rapid method of thermally bonding cover plates to polymer substrates
containing fluid networks (pressure assisted, boiling point thermal fusion bonding (PABP TFB)) will be used
that delivers high process yield rates. Another challenge for building modular systems is to ensure that the
small quantities of target DNA, RNA, or proteins can pass reliably between modules and the motherboard
without loss and eliminating the need for O-rings or gaskets. To eliminate the need for gaskets, the
modular systems will use gasketless superhydrophobic fluid interconnects that combine precision
alignment of components to set gaps <10 µm, with superhydrophobic films on the surfaces around a fluid
passage. The seal is a function of capillary forces, has a predictable failure pressure, and can transport
biofluids, such as plasma and blood, and reagents from module to motherboard. The gasketless interconnect
is tolerant of misalignment, which occurs in normal manufacturing processes; efforts in the renewal application
will establish what the range is and the maximum failure pressure.
To demonstrate the power of the modular fluidic systems, TR&D 3 will manufacture and assemble a
complete modular system that will enrich circulating tumor cells (CTCs) and sequence certain genes in their
genome. The system will have five modules connected to a universal motherboard and process a blood sample
to an electronic readout of selected genes of the CTCs, providing actionable information for clinicians.
题目:精准医学生物模块化多尺度系统(CBM2)生物技术资源中心
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MICHAEL C. MURPHY其他文献
MICHAEL C. MURPHY的其他文献
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{{ truncateString('MICHAEL C. MURPHY', 18)}}的其他基金
Manufacture and Assembly of Thermoplastic, Modular, Integrated Fluidic Systems
热塑性、模块化、集成流体系统的制造和组装
- 批准号:
10693393 - 财政年份:2015
- 资助金额:
$ 19.07万 - 项目类别:
Manufacture and Assembly of Thermoplastic, Modular, Integrated Fluidic Systems
热塑性、模块化、集成流体系统的制造和组装
- 批准号:
10493140 - 财政年份:2015
- 资助金额:
$ 19.07万 - 项目类别:
Biotechnology Resource Center of Biomodular Multi scale Systems CBM2 for Precision Molecular Diagnostics
用于精密分子诊断的生物模块化多尺度系统 CBM2 生物技术资源中心
- 批准号:
9145225 - 财政年份:
- 资助金额:
$ 19.07万 - 项目类别:
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