Wadsworth Center's Radiochemical, Microbiological and Chemical Testing for Food Defense and Capability Development (Discipline A: Microbiology, Analytical Track 1: Food Defense and Maintenance)

沃兹沃斯中心的食品防护和能力开发放射化学、微生物和化学测试（学科 A：微生物学，分析轨道 1：食品防护和维护）

• 批准号: 10173138
• 负责人: Christina Egan
• 金额: $ 16.71万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10173138
• 关键词: Wadsworth; Center; Radiochemical; Microbiological; Chemical

ABSTRACT The goal of this project is to enhance the ability to analyze for microbiological threat agents utilizing FERN and other methods to improve laboratory capacities for food defense assignments and outbreak response. As a Tier 1 Select Agent registered facility with both Biosafety Level 2 (BSL-2) and Biosafety Level 3 (BSL-3) laboratories, our highly experienced staff is capable of performing more than 500 analyses per month for biothreat agents and toxins. The Wadsworth Center plays an active role in food testing in NYS. Our laboratory is involved with food outbreak investigations, routine food surveillance, and a resource for confirmatory testing for other state and federal agencies involved in the regulation of food. We perform identification and characterization on organisms isolated from human specimens as well as associated implicated food sources using methods such as classical microbiological methods, in-house developed rtPCR, DNA sequencing, molecular strain and serotyping, and DNA fingerprinting. In addition to the FERN and LRN, the Wadsworth Center also participates in the Emerging Infectious Program funded by the CDC which includes FoodNet, PulseNet, and the National Resistance Monitoring System. The Wadsworth Center works very closely with the NYS DOH Bureau of Communicable Disease Control and Bureau of Food Protection as well as other state and federal partners including Department of Agriculture and Markets and the FDA and USDA to assist in providing expertise in the isolation and identification of select agent microbes and toxins in food products and a timely, coordinated response to food related outbreaks. Staff in the Biodefense Laboratory have attended FERN sponsored trainings as well as developed and delivered several trainings as subject matter experts. The most recent training that was led by members of our laboratory was a MALDI-TOF MS applications course for biothreat agents and toxins. The MALDI-TOF MS methods are being adopted by many state and federal agricultural and public health laboratories. Our laboratory is currently assisting other state and federal agencies validate and implement these new methods in their laboratories as well as participate in a monthly Clostridium botulinum harmonization conference call with members of the FDA, USDA, and CDC where we are the only public health laboratory member. The expertise of our laboratorians has allowed for the development and validation of multiple new methods that are currently being used to screen food commodities for high risk pathogens and toxins. The expected outcome of this project is to ensure that FDA distributed food commodities are safe to consume for the general public and to provide enhanced capabiity and capacity for FERN. Additionally, it is expected that the food supply will be safe from intentional contamination during large gatherings and events, such as political conventions, inaugurations, and the Super Bowl.

摘要 该项目的目标是提高利用FERN分析微生物威胁因子的能力， 其他方法，以提高食品防御任务和疫情反应的实验室能力。作为 一级生物安全等级2（BSL-2）和生物安全等级3（BSL-3）的精选代理注册机构 我们经验丰富的员工每月能够进行500多项分析， 生物威胁剂和毒素 沃兹沃斯中心在纽约州的食品检测中发挥着积极的作用。我们的实验室与食品有关 疫情调查、常规食品监测和其他州的确证性检测资源， 参与食品管理的联邦机构。我们对以下对象进行识别和表征 从人类样本以及相关的相关食物来源中分离的微生物， 作为经典的微生物学方法，内部开发的rtPCR、DNA测序、分子菌株和 血清分型和DNA指纹分析除了FERN和LRN，沃兹沃斯中心也参与 在疾病预防控制中心资助的新兴传染病计划中，包括FoodNet，PulseNet和国家 电阻监测系统。沃兹沃斯中心与纽约州卫生署密切合作， 传染病控制和食品保护局以及其他州和联邦合作伙伴 包括农业和市场部以及FDA和USDA，以协助提供 隔离和鉴定食品中的特定病原微生物和毒素， 应对与食品有关的疫情。 生物防御实验室的工作人员参加了欧洲环境研究网络主办的培训， 作为专题专家提供了几次培训。最近的一次培训是由我们的 实验室是一个MALDI-TOF MS生物威胁剂和毒素的应用课程。MALDI-TOF MS 许多州和联邦农业和公共卫生实验室正在采用这些方法。我们 实验室目前正在协助其他州和联邦机构验证和实施这些新方法， 他们的实验室，以及参加每月肉毒梭菌协调电话会议， FDA，USDA和CDC的成员，我们是唯一的公共卫生实验室成员。的专门知识 我们的实验室人员已经允许开发和验证多种新方法， 用于筛查食品中的高风险病原体和毒素。 这一项目的预期成果是确保林业发展局分发的食品可以安全消费 并为FERN提供增强的能力和容量。此外，预计 在大型聚会和活动期间，食品供应将是安全的，不会受到故意污染， 政治大会就职典礼和超级碗