Microbiology Capability/Capacity Development

项目摘要 (微生物学：能力/能力发展、全基因组测序) 新泽西州农业部(NJDA)实验室的目标是参与 微生物学能力/能力发展项目，是开发下一代测序 (NGS)食源性病原体和相关兽医临床分离株的能力。NJDA 微生物计划有包括设施、设备和人员在内的资源来分离 样品中的致病微生物。NJDA微生物学计划缺乏设备 完成全基因组测序所需的。为此目的，NJDA希望 购买Illumina ISEQ 100新一代测序仪、试剂、耗材和 软件，并获得全基因组测序(WGS)方面的培训。微生物测序研究进展 ISEQ 100是集成工作流系统的一部分，该系统由图书馆准备和 NextEra DNA Flex Library准备试剂盒、Illumina测序和按钮数据分析 基地空间序列枢纽。实验室人员将参加在线和现场WGS，并 生物信息学培训，并通过初步能力评估，然后继续进行 WGS。工作人员还将参加GenomeTrakr熟练程度测试并参加年度 GenomeTrakr和面对面的会面。新一代测序仪的收购以及 所有的用品、消耗品和人员将极大地提高NJDA实验室的能力 和国家能力。

Project Summary (Microbiology: Capability/Capacity Development, Whole Genome Sequencing) The New Jersey Department of Agriculture (NJDA) laboratory’s aim, in participating in the microbiology capability/capacity development project, is to develop next generation sequencing (NGS) capability of food borne pathogens and related veterinary clinical isolates. The NJDA microbiology program has resources including facility, equipment, and personnel to isolate pathogenic microorganisms from samples. The NJDA microbiology program lacks equipment required to accomplish the whole genome sequencing. To this end, the NJDA would like to purchase an Illumina iSEQ 100 next generation sequencer, reagents, consumables, and software, and obtain training in whole genome sequencing (WGS). Microbial sequencing on the iSEQ 100 is part of an integrated workflow system that consists of library preparation with the Nextera DNA Flex Library preparation kit, Illumina sequencing, and push button data analysis in Base Space Sequence hub. Laboratory personnel will attend online and onsite WGS and bioinformatics trainings and pass an initial competency assessment before proceeding to do the WGS. Personnel will also participate in GenomeTrakr proficiency tests and attend the annual GenomeTrakr and face-to-face meeting. The acquisition of next generation sequencer along with all supplies, consumables, and personnel will greatly enhance the NJDA laboratory’s capability and the national capacity.