Microbiology Capability/Capacity Development

项目摘要 （微生物学：能力/能力发展，全基因组测序） 新泽西农业部（NJDA）实验室的目标是， 微生物学能力/能力开发项目，是开发下一代测序 (NGS)食源性病原体和相关兽医临床分离株的能力。NJDA 微生物学项目拥有包括设施、设备和人员在内的资源， 从样品中分离病原微生物。NJDA微生物学项目缺乏设备 完成全基因组测序所需的所有基因。为此，NJDA希望 购买Illumina iSEQ 100下一代测序仪、试剂、耗材， 软件，并获得全基因组测序（WGS）的培训。微生物测序 iSEQ 100是集成工作流程系统的一部分，该系统包括文库制备和 Nextera DNA Flex文库制备试剂盒、Illumina测序和按钮数据分析， 基地空间序列枢纽。实验室人员将参加在线和现场WGS， 生物信息学培训，并通过初步能力评估，然后再继续进行 WGS人员还将参加GenomeTrakr能力测试并参加年度 GenomeTrakr和面对面会议。下一代测序仪的获得沿着 所有的供应品、消耗品和人员将大大提高NJDA实验室的能力 和国家能力。

Project Summary (Microbiology: Capability/Capacity Development, Whole Genome Sequencing) The New Jersey Department of Agriculture (NJDA) laboratory’s aim, in participating in the microbiology capability/capacity development project, is to develop next generation sequencing (NGS) capability of food borne pathogens and related veterinary clinical isolates. The NJDA microbiology program has resources including facility, equipment, and personnel to isolate pathogenic microorganisms from samples. The NJDA microbiology program lacks equipment required to accomplish the whole genome sequencing. To this end, the NJDA would like to purchase an Illumina iSEQ 100 next generation sequencer, reagents, consumables, and software, and obtain training in whole genome sequencing (WGS). Microbial sequencing on the iSEQ 100 is part of an integrated workflow system that consists of library preparation with the Nextera DNA Flex Library preparation kit, Illumina sequencing, and push button data analysis in Base Space Sequence hub. Laboratory personnel will attend online and onsite WGS and bioinformatics trainings and pass an initial competency assessment before proceeding to do the WGS. Personnel will also participate in GenomeTrakr proficiency tests and attend the annual GenomeTrakr and face-to-face meeting. The acquisition of next generation sequencer along with all supplies, consumables, and personnel will greatly enhance the NJDA laboratory’s capability and the national capacity.