Genetic Relatedness and Genomic Characteristics of Vibrio Species Found in Oysters and Clams Harvested for Human Consumption

申请名称：新泽西食品测试计划-食品安全和国防（2020-2025） 项目名称：在蚝和蚬中发现的弧菌的遗传相关性及基因组特征 收获供人类消费 项目概要/摘要-学科A：微生物学，分析轨道4：全基因组测序 新泽西卫生部（NJDOH）正在寻求美国食品和药物管理局（FDA）的 实验室灵活供资模式合作协议，重点是提高实验室的能力 新泽西州维护食品安全和保障。肠道、食源性和全基因组测序 NJDOH的公共卫生实验室服务（PHLS）微生物学计划。该项目旨在提高 致病性弧菌的遗传特性的了解。蛤和牡蛎中发现的 这些贝类是人类食源性疾病的常见来源，由于其特定的环境， 特征及其消费频率。这些贝类是在特拉华州海湾收获的 和新泽西州的大西洋海岸沃茨，并在整个夏季采样检测弧菌属（弧菌 创伤弧菌和副溶血性弧菌）。通常，从收获的分离物中回收40-50个分离物。 每年夏天都吃贝类。过去收获季节的细菌分离株已保存并冷冻储存。 其中约100个分离株将被复活，并进行DNA分离和全基因组测序。 这些基因组序列将被上传到国家数据库，迄今为止，这些数据库中只有很少的弧菌基因组 包括，尽管近年来人类发病率的病例增加。这些基因组的加入 将有助于扩大对这些贝类中发现的弧菌属物种的遗传特征的了解， 增强检测人类样本中分离株之间遗传相关性的能力。这些数据将 还允许在各种环境设置中发现的分离株之间进行比较。最后，指导和/或 将向其他成员实验室提供全基因组测序过程的培训， 这一重要的实验室工具的扩展。 该项目将有助于大大扩大对粮食供应新出现的威胁的了解， 水生食物来源。通过更好地了解贝类弧菌污染背后的科学，NJDOH， 国家合作伙伴和FDA将能够更好地保障食品供应。

Application Title: New Jersey Food Testing Program – Food Safety and Defense (2020-2025) Project Title: Genetic Relatedness and Genomic Characteristics of Vibrio Species Found in Oysters and Clams Harvested for Human Consumption Project Summary/Abstract – Discipline A: Microbiology, Analytical Track 4: Whole Genome Sequencing The New Jersey Department of Health (NJDOH) is pursuing the U.S. Food and Drug Administration’s (FDA) Laboratory Flexible Funding Model cooperative agreement focused on improving the capability and capacity of NJ to maintain food safety and security. The Enterics, Foodborne, and Whole Genome Sequencing is housed within NJDOH’s Public Health Laboratory Services (PHLS) Microbiology Program. This project aims to enhance the understanding of genetic characteristics of pathogenic Vibrio spp. found in clams and oysters. These shellfish are a common source of foodborne illness in humans, due to their specific environmental characteristics and the frequency of their consumption raw. These shellfish are harvested in the Delaware Bay and Atlantic Coast waters of NJ and sampled throughout the summer months for detection of Vibrio spp (Vibrio vulnificus and Vibrio parahaemolyticus). Typically, between 40-50 isolates are recovered from harvested shellfish each summer. Bacterial isolates from past harvesting seasons have been saved and stored frozen. Approximately 100 of these isolates will be revived and undergo DNA isolation and whole genome sequencing. The genomic sequences will be uploaded to national databases, which to date have very few Vibrio genomes included, despite the increase in cases of human pathogenesis in recent years. The addition of these genomes will help expand understanding of genetic characteristics of Vibrio spp that are found in these shellfish and enhance the ability to detect genetic relatedness between isolates found in human specimens. These data will also allow comparisons between isolates found in various environmental settings. Finally, mentorship and/or training will be provided to other member laboratories in the processes of whole genome sequencing to aid in the expansion of this important laboratory tool. This project will serve to significantly expand the understand of an emerging threat to the food supply from aquatic food sources. By better understanding the science behind Vibrio contamination of shellfish, NJDOH, its State partners, and FDA will be able to better safeguard the food supply.