Genetic Relatedness and Genomic Characteristics of Vibrio Species Found in Oysters and Clams Harvested for Human Consumption

申请标题：新泽西州食品检测计划--食品安全与防御(2020-2025年) 项目名称：牡蛎和文蛤中弧菌的遗传关系和基因组特征 为人类消费而收获 项目摘要/摘要-学科A：微生物学，分析轨道4：全基因组测序 新泽西州卫生部(NJDOH)正在追查美国食品和药物管理局(FDA) 实验室灵活资助模式合作协议侧重于提高能力和能力 新泽西州要维护食品安全和保障。肠道、食源性和全基因组测序 在NJDOH公共卫生实验室服务(PHLS)微生物学计划中。该项目旨在增强 对致病性弧菌遗传特性的认识。见于文蛤和牡蛎中。 由于其特殊的环境，这些贝类是人类食源性疾病的常见来源。 他们的特点和消费生鲜的频率。这些贝类是在特拉华湾捕捞的 和新泽西州的大西洋沿岸水域，并在整个夏季几个月进行采样，以检测弧菌(Vibrio 创伤弧菌和副溶血性弧菌)。通常，从收获的样品中回收40-50个分离物。 每年夏天的贝类。过去收获季节的细菌分离株已被保存并冷冻保存。 其中大约100个分离株将被复活，并接受DNA分离和全基因组测序。 基因组序列将被上传到国家数据库，到目前为止，这些数据库中几乎没有弧菌基因组 包括在内，尽管近年来人类发病病例有所增加。这些基因组的增加 将有助于扩大对这些贝类和贝类中发现的弧菌遗传特征的了解 提高检测在人类样本中发现的分离株之间遗传联系的能力。这些数据将 还可以对不同环境中发现的分离株进行比较。最后，导师和/或 将向其他成员实验室提供全基因组测序过程方面的培训，以帮助 这一重要实验室工具的扩展。 这一项目将大大扩大对粮食供应新威胁的了解。 水产食物来源。通过更好地了解弧菌污染贝类背后的科学，NJDOH，其 国家合作伙伴和FDA将能够更好地保障食品供应。

Application Title: New Jersey Food Testing Program – Food Safety and Defense (2020-2025) Project Title: Genetic Relatedness and Genomic Characteristics of Vibrio Species Found in Oysters and Clams Harvested for Human Consumption Project Summary/Abstract – Discipline A: Microbiology, Analytical Track 4: Whole Genome Sequencing The New Jersey Department of Health (NJDOH) is pursuing the U.S. Food and Drug Administration’s (FDA) Laboratory Flexible Funding Model cooperative agreement focused on improving the capability and capacity of NJ to maintain food safety and security. The Enterics, Foodborne, and Whole Genome Sequencing is housed within NJDOH’s Public Health Laboratory Services (PHLS) Microbiology Program. This project aims to enhance the understanding of genetic characteristics of pathogenic Vibrio spp. found in clams and oysters. These shellfish are a common source of foodborne illness in humans, due to their specific environmental characteristics and the frequency of their consumption raw. These shellfish are harvested in the Delaware Bay and Atlantic Coast waters of NJ and sampled throughout the summer months for detection of Vibrio spp (Vibrio vulnificus and Vibrio parahaemolyticus). Typically, between 40-50 isolates are recovered from harvested shellfish each summer. Bacterial isolates from past harvesting seasons have been saved and stored frozen. Approximately 100 of these isolates will be revived and undergo DNA isolation and whole genome sequencing. The genomic sequences will be uploaded to national databases, which to date have very few Vibrio genomes included, despite the increase in cases of human pathogenesis in recent years. The addition of these genomes will help expand understanding of genetic characteristics of Vibrio spp that are found in these shellfish and enhance the ability to detect genetic relatedness between isolates found in human specimens. These data will also allow comparisons between isolates found in various environmental settings. Finally, mentorship and/or training will be provided to other member laboratories in the processes of whole genome sequencing to aid in the expansion of this important laboratory tool. This project will serve to significantly expand the understand of an emerging threat to the food supply from aquatic food sources. By better understanding the science behind Vibrio contamination of shellfish, NJDOH, its State partners, and FDA will be able to better safeguard the food supply.