Whole Genome Sequencing

FDA食品安全和应用营养中心（CFSAN）创建了首个 州和联邦实验室的综合网络（GenomeTrakr）， 测序追踪食源性病原体，以改善疫情应对和有效 预防性控制的监测。GenomeTrakr网络创造了一个 全球数据库，包含数千种食源性疾病的基因组成 导致有机体。该项目旨在发展一个更强大的国际快速 通过进一步加强GenomeTrakr网络建立病原体追踪监测系统 不断增长的数据库。准确及时的分型和随后的聚类 与食源性爆发事件相关的细菌分离物对于成功地 调查并最终追溯到特定的食品或环境来源。基因组 GenomeTrakr网络提供的信息将调查人员指向特定的食品 可能与疫情有关，并提供了对受污染食品来源的深入了解。 该项目将促进公共卫生和国家农业实验室序列病原 将食源性细菌添加到在线数据库中。当地实验室将被要求 为最少250个食物、环境或动物分离物进行测序， 数据库，以确定未来的爆发。当地实验室也将被要求参加 GenomeTrakr在标准过程中需要测序和分析的一致性， 还参加各种会议和能力测试，以确保标准化 实验室之间。

The FDA’s Center for Food Safety and Applied Nutrition (CFSAN) has created the first integrated network of state and federal laboratories (GenomeTrakr) to use whole genome sequencing to track foodborne pathogens to improve outbreak response and effective monitoring of preventative controls. The GenomeTrakr network has created a publically available, global database containing the genetic makeup of thousands of foodborne disease causing organisms. This project is intended to develop a stronger international rapid surveillance system for pathogen trace-back by further enhancing the GenomeTrakr network and the growing database. The accurate and timely subtyping and subsequent clustering of isolates of a bacterium associated with a foodborne outbreak event is essential for successful investigation and eventual trackback to a specific food or environmental source. The genomic information provided by the GenomeTrakr network points investigators to specific food products potentially related to an outbreak, and provides insight into the origin of the contaminated food. This project will promote public health and state agricultural laboratories sequence pathogenic foodborne bacterium to be added to the on-line database. The local laboratories will be required to sequence a minimum of 250 food, environmental or animal isolates to be added to the database to identify future outbreaks. The local laboratories will also be required to participate in GenomeTrakr in a standard process requiring consistency of the sequencing and analysis while also participating in meetings, conferences and proficiency tests to ensure standardization between laboratories.