IDALS LFFM Special Projects Method Development Validation

特殊项目-方法开发/验证 该项目是一个矩阵扩展单一实验室验证，将扩大测试化学 有害物质（杀虫剂）进入动物饲料。IDALS一直在使用一种改进的多残基方法，该方法基于 QuEChERS萃取-高效液相色谱-串联质谱联用 使用质谱法（LC-MS/MS）检测酸性除草剂的残留水平。QuEChERS方法具有 提取和清理步骤，已开发为快速，简单，便宜，高效，坚固耐用 和安全.最初，修改后的方法被验证用于植物基质，但从那时起， 成功地适用于土壤和拭子，回收率相当高。随着 鉴于检测饲料中除草剂残留的重要性，IDALS建议扩大这一多方面的范围， 通过执行二级单一实验室进行基质扩展的农药残留方法 验证酸性除草剂与动物饲料。一种检测动物体内农药残留的方法 饲料将支持FDA努力减少这两种动物对危险化学品的食源性暴露 还有人类

Project Summary Special Projects – Method Development/Validation This project is a matrix extension single laboratory validation that will expand testing for chemical hazards (pesticides) into animal feed. IDALS has been using a modified multi-residue method, based on QuEChERS extraction coupled with high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) for detecting residue levels of acid herbicides. The QuEChERS method has extraction and clean-up steps, which have been developed to be Quick, Easy, Cheap, Efficient, Rugged and Safe. Originally, the modified method was validated for plant matrices but since then has been successfully adapted for soil and swabs with reasonably high recovery rates. With the surge of importance of detecting herbicide residues in feed, IDALS proposes to extend the scope of this multi- residue pesticide method by conducting a matrix extension by executing a level two single laboratory validation for acid herbicides with animal feed. A method to detect the presence of pesticides in animal feeds will support FDA efforts to minimize foodborne exposure to hazardous chemicals for both animals and humans.