IDALS LFFM Special Projects Method Development Validation

项目摘要特殊项目-方法开发/验证 该项目是一个矩阵扩展单一实验室验证，将扩大对化学品的测试 动物饲料中的有害物质(农药)。IDALS一直在使用一种改进的多残基方法，其基础是 QuEChERS萃取-高效液相色谱-串联质谱仪联用 用LC-MS/MS法检测酸性除草剂的残留量。QuEChERS方法具有 提取和清理步骤，已被开发为快速、容易、廉价、高效、坚固 而且很安全。最初，修改后的方法被验证用于植物矩阵，但自那以来一直是 成功地适应了土壤和棉签，具有相当高的回收率。随着……的激增 鉴于检测饲料中除草剂残留的重要性，IDALS建议扩大这一多项技术的范围。 一种通过执行二级单一实验室进行基质延伸的残留农药方法 动物饲料中酸性除草剂的验证。一种检测动物体内农药残留的方法 饲料将支持FDA将两只动物的食源性危险化学品暴露降至最低的努力 和人类。

Project Summary Special Projects – Method Development/Validation This project is a matrix extension single laboratory validation that will expand testing for chemical hazards (pesticides) into animal feed. IDALS has been using a modified multi-residue method, based on QuEChERS extraction coupled with high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) for detecting residue levels of acid herbicides. The QuEChERS method has extraction and clean-up steps, which have been developed to be Quick, Easy, Cheap, Efficient, Rugged and Safe. Originally, the modified method was validated for plant matrices but since then has been successfully adapted for soil and swabs with reasonably high recovery rates. With the surge of importance of detecting herbicide residues in feed, IDALS proposes to extend the scope of this multi- residue pesticide method by conducting a matrix extension by executing a level two single laboratory validation for acid herbicides with animal feed. A method to detect the presence of pesticides in animal feeds will support FDA efforts to minimize foodborne exposure to hazardous chemicals for both animals and humans.