ICP-QQQ

ICP-MS/MS

• 批准号: 10175879
• 负责人: Danielle Mary Jorgens
• 金额: $ 159.09万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2023-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175879
• 关键词: 3-Dimensional; Biogenesis; Biological; Cellular Structures; Cellular biology; Cilia; Communities; Complex; Cryo-electron tomography; Cryoelectron Microscopy; Electron Microscope; Electron Microscopy; Faculty; Functional disorder; Funding; Image; In Situ; Infrastructure; Investments; Ions; Laboratories; Length; Mainstreaming; Methodology; Methods; Microscope; Minor; Modernization; Molecular Biology; Molecular Structure; Nutrient; Organelles; Plant Resins; Research Personnel; Research Project Grants; Resolution; Sampling; Scanning; Signal Transduction; Structure; System; Temperature; Titan; Viral Pathogenesis; Virus Replication; Visualization; Work; cellular imaging; complex biological systems; experience; instrument; instrumentation; millimeter; particle; structural biology; tumorigenesis; uptake

Project Summary Recent methodological and technological advances have resulted in a rapid expansion of electron microscopy (EM) applications at UC Berkeley and in the wider scientific community. In particular, single particle cryo-EM applications have led to numerous high-profile advances in the field of structural biology. The equally compelling parallel fields of cryo-electron tomography (cryo-ET) and volume EM, both enabled by advances in focused ion beam (FIB) milling, have become increasingly mainstream in the study of complex biological systems. These methods allow for the direct high-resolution visualization of macromolecular and cellular structures within a native context, bridging the fields of cell biology and structural biology in an unprecedented manner. The UC Berkeley electron microscopy and cell biology communities, long at the forefront of their respective fields, have embarked upon a sustained investment in combining these promising in situ EM visualization methods. As research projects grounded in these methods have begun to mature, the need for our faculty to access cutting-edge instrumentation has become both urgent and abundantly clear. Currently, there exists no high-end cryo-capable FIB scanning electron microscope (cryo-FIB-SEM) accessible to researchers on our campus with complex, biological samples. In this proposal, we request funds to purchase a cryo-capable Zeiss Crossbeam (XB) 550 FIB-SEM and ancillary cryo-instrumentation. The cutting-edge capabilities of this instrument will greatly advance efforts at UC Berkeley to characterize biological structures across many orders of magnitude, from angstrom to millimeter length scales. The Zeiss XB 550 FIB-SEM microscope enables the determination of 3D volume-EM imaged cellular structures at both room (3 nm resolution, resin-embedded) and cryo (30 nm resolution) temperatures. With its cutting-edge capabilities in cryo-FIB milling, the Zeiss XB 550 will also be seamlessly integrated into the campus cryo-pipeline to generate cellular cryo-lamellae for later high resolution cryo-ET imaging using Berkeley’s two existing Titan Krios cryo-electron microscopes. Acquisition of the proposed system will greatly impact the work of three Major User and five Minor User groups, which are focused on important biological and biomedical questions regarding viral replication and pathogenesis, cellular signaling and nutrient uptake, oncogenesis, cilia dysfunction, and organelle biogenesis. The UC Berkeley Electron Microscopy Laboratory, expertly staffed by experienced biological microscopists who have served campus academic researchers for decades, will house the Zeiss XB 550. Our acquisition of the Zeiss XB 550 will completely modernize our EM infrastructure, accelerating UC Berkeley’s progress on a sustainable path to drive fundamental breakthroughs in cell and molecular biology.

项目概要 最近的方法和技术进步导致电子显微镜的快速扩展 （EM）在加州大学伯克利分校和更广泛的科学界的应用。特别是，单粒子冷冻电镜 应用已在结构生物学领域带来了许多备受瞩目的进展。同样引人注目的 冷冻电子断层扫描 (cryo-ET) 和体积 EM 的平行场，均通过聚焦离子的进步实现 光束（FIB）铣削已成为复杂生物系统研究中越来越主流的技术。这些 方法允许直接高分辨率可视化天然体内的大分子和细胞结构 背景下，以前所未有的方式弥合细胞生物学和结构生物学领域。加州大学伯克利分校 电子显微镜和细胞生物学界长期处于各自领域的最前沿，已经开始 基于对这些有前景的原位电磁可视化方法的持续投资。作为研究 基于这些方法的项目已经开始成熟，我们的教师需要接触尖端技术 仪器仪表已变得既紧迫又非常明确。目前，还没有高端的低温技术 FIB 扫描电子显微镜 (cryo-FIB-SEM) 供我们校园的研究人员使用，具有复杂、 生物样本。在此提案中，我们请求资金购买具有低温功能的 Zeiss Crossbeam (XB) 550 FIB-SEM 和辅助冷冻仪器。该仪器的尖端功能将大大提高 加州大学伯克利分校致力于表征多个数量级的生物结构，从埃到 毫米长度刻度。 Zeiss XB 550 FIB-SEM 显微镜能够确定 3D 体积-EM 在室温（3 nm 分辨率，树脂嵌入）和低温（30 nm 分辨率）下对细胞结构进行成像 温度。凭借其在冷冻 FIB 铣削领域的尖端功能，Zeiss XB 550 也将无缝地 集成到校园冷冻管道中，生成细胞冷冻片层，用于以后的高分辨率冷冻电子断层扫描 使用伯克利现有的两台 Titan Krios 冷冻电子显微镜进行成像。收购拟议系统 将极大地影响三个主要用户组和五个次要用户组的工作，这些用户组专注于重要的 有关病毒复制和发病机制、细胞信号传导和营养的生物学和生物医学问题 摄取、肿瘤发生、纤毛功能障碍和细胞器生物发生。加州大学伯克利分校电子显微镜 实验室由经验丰富的生物显微镜专家组成，他们曾为校园学术服务过 研究人员几十年来，将配备蔡司 XB 550。我们对蔡司 XB 550 的收购将完全 实现我们的新兴市场基础设施现代化，加速加州大学伯克利分校在可持续发展道路上的进步 细胞和分子生物学的根本性突破。

项目成果

Crystalline Polyphenylene Covalent Organic Frameworks.

• DOI: 10.1021/jacs.3c11688
• 发表时间: 2023-12
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Xing Han;Zihui Zhou;Kaiyu Wang;Zhiling Zheng;S. E. Neumann;Heyang Zhang;Tianqiong Ma;O. Yaghi-O

Directing Molecular Weaving of Covalent Organic Frameworks and Their Dimensionality by Angular Control.

• DOI: 10.1021/jacs.3c09691
• 发表时间: 2023-10
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Xing Han;S. E. Neumann;B. Nannenga;Kaiyu Wang;Kam-Yun Li;Saber Mirzaei;Xuan Yao;Chenhui Zhu;Mei-Yan Gao;Yue-Biao Zhang;Yong Cui;O. Yaghi;Kelvin Kam-Yun