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Leica SP8 Confocal Microscope

徕卡 SP8 共焦显微镜

基本信息

批准号：
10175244
负责人：
David Antonetti
金额：
$ 59.91万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2022-03-31
项目状态：
已结题

项目摘要

PROJECT DESCRIPTION This application requests funds to purchase a new confocal microscope to support the imaging needs of over 30 investigators at the University of Michigan. The Brehm and Kellogg Research towers of the Kellogg Eye Center house both Diabetes and Vision research scientists that have worked collaboratively to achieve high quality imaging for over 10 years with support from the NIDDK-funded Michigan Diabetes Research Center and the NEI-funded Vision Research Core. These investigators share space in the Brehm Research tower and have established a shared Imaging Facility that supports tissue and cellular processing and imaging with a strong emphasis on confocal microscopy. However, two of the three confocal microscopes are now over 10 years old and unable to provide the imaging requirements of our investigators. We propose to purchase a new confocal microscope to replace two of the existing microscopes based on four major needs common to this group of investigators. First, the system provides high speed resonant scanning to produce tiled images of large organ structures with significant improvement in image resolution compared to currently available systems. Second, the new system possesses a broad spectrum of excitation and emission capabilities with a high degree of precision to allow specific signal detection. Third, the system provides the ability to reduce background autofluorescence through time gating, a feature required by a number of our Imaging Facility users. Fourth, the system will be outfitted for live cell imaging to provide quantitative live cell assays. The new microscope will support a large group of Major Users with NIH funding addressing such fundamental biological issues as central control of feeding behavior and retinal light detection and neural circuitry as well as fundamental cell biological problems such as ion channel function and distribution, cilia formation and cell division. Other Major Users study disease pathology, including diabetes, diabetic retinopathy and neuropathy, age related macular degeneration and glaucoma. The new microscope will integrate into the shared Imaging Facility with a well-established infrastructure for providing service, training, and long-term support. Finally, the University of Michigan will provide substantial support to maintain and further equip the requested confocal microscope and the Imaging Facility. Collectively, the requested purchase will be part of a larger effort to provide imaging support to advance NIH funded research improving human health.

项目描述该申请要求资金购买一台新的共聚焦显微镜，以支持30多个国家的成像需求。密歇根大学的研究人员。凯洛格眼科中心的布鲁姆和凯洛格研究塔容纳糖尿病和视力研究科学家，他们共同努力，在NIDDK资助的密歇根糖尿病研究中心和 NEI资助的视觉研究核心。这些研究人员在Bengym研究塔共享空间，建立了一个共享的成像设施，支持组织和细胞的处理和成像，重点是共聚焦显微镜。然而，三台共焦显微镜中的两台现在已经超过10年了无法提供我们调查人员的影像学要求。我们建议购买一台新的共焦显微镜，以取代两个现有的显微镜的基础上，四个主要的共同需要，这组 investigators.首先，系统提供高速共振扫描以产生大器官的平铺图像与当前可用的系统相比，该结构在图像分辨率方面具有显著的改进。第二、新系统具有广泛的激发和发射能力，精确度，以允许特定的信号检测。第三，系统提供了减少背景的能力通过时间门控自动荧光，这是我们的成像设备用户所需的功能。四是系统将配备活细胞成像，以提供定量活细胞分析。新的显微镜将用NIH的资金支持一大群主要用户，解决诸如核心的基本生物学问题，控制进食行为和视网膜光检测和神经回路以及基本细胞生物学离子通道功能和分布、纤毛形成和细胞分裂等问题。其他主要用户研究疾病病理学，包括糖尿病、糖尿病性视网膜病变和神经病、年龄相关性黄斑变性和青光眼。新的显微镜将整合到共享的成像设施，提供服务、培训和长期支持的基础设施。最后，密歇根大学将提供为维护和进一步装备所要求的共聚焦显微镜和成像设施提供实质性支持。总的来说，所要求的购买将是提供成像支持以推进NIH的更大努力的一部分资助研究改善人类健康。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Obesity-induced neuroinflammation and cognitive impairment in young adult versus middle-aged mice.

DOI：
10.1186/s12979-022-00323-7
发表时间：
2022-12-22
期刊：
IMMUNITY & AGEING
影响因子：
7.9
作者：
Henn, Rosemary E.;Elzinga, Sarah E.;Glass, Emily;Parent, Rachel;Guo, Kai;Allouch, Adam A.;Mendelson, Faye E.;Hayes, John;Webber-Davis, Ian;Murphy, Geoffery G.;Hur, Junguk;Feldman, Eva L.
通讯作者：
Feldman, Eva L.

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures.

改进的脂褐质模型和高度极化人视网膜色素上皮培养物中外节吞噬能力的量化。