ShEEP Request for Zeiss Modular Laser Scanning Microscope LSM 980

ShEEP 请求蔡司模块化激光扫描显微镜 LSM 980

• 批准号: 10175791
• 负责人: Gregory A. Elder
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175791
• 关键词: 3-Dimensional; Alzheimer' s Disease; Area; Core Facility; Detection; Disease; Environment; Equipment; Goals; Health; Image; Imaging Techniques; Imaging technology; Lasers; Malignant Neoplasms; Medical center; Mental disorders; Microscope; Microscopy; Performance; Program Development; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Sheep; Spinal cord injury; System; Training; Traumatic Brain Injury; Veterans; confocal imaging; in vivo imaging; multiphoton imaging; multiphoton microscopy; operation; optical imaging; research and development

The Research & Development Program at the James J. Peters VA Medical Center (JJPVAMC) has for many years supported a variety of core equipment items that are available to VA investigators and their collaborators. Among the most utilized core equipment items are a set of microscopes that function as part of a Microscopy Core Facility. Central to the operations of this Microscopy Core is a Zeiss LSM 700 microscope that was purchased 7 years. This microscope has been a workhorse that has provided JJPVAMC researches with high-resolution laser scanning confocal imaging for many years. However, with advances in imaging technology, this microscope is now lacks the sensitivity and important functionalities that are essential for high performance confocal/multiphoton imaging and in vivo imaging applications. The goal of this application is to replace an outdated Zeiss LSM700 microscope with an optimally configured and fully functional confocal/multiphoton microscope system that will be be made available to JJPVAMC investigators. We propose to replace the LSM 700 system with a Zeiss LSM 980 confocal/multiphoton inverted laser-scanning microscope with Airyscan detection for 3D super-resolution imaging. The LSM 980 confocal/multiphoton system will be placed in a multiuser environment. Technical support as well user training will be provided to support all VA research projects that might benefit from high-end imaging. Acquisition of an LSM 980 confocal/multiphoton system will provide a cutting-edge system for high resolution laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques.

詹姆斯·J·彼得斯退伍军人医学中心(JJPVAMC)的研究和开发计划 多年来为退伍军人管理局调查人员和他们的 合作者。最常用的核心设备之一是一套显微镜，它的功能是 显微镜核心设备。该显微镜核心的操作中心是蔡司LSM 700显微镜 那是7年前买的。这台显微镜一直是JJPVAMC研究的主力 用高分辨率激光扫描共聚焦成像已有多年。然而，随着成像技术的进步 技术，这种显微镜现在缺乏灵敏度和重要的功能，这是必不可少的高 高性能共焦/多光子成像和活体成像应用。此应用程序的目标是 将过时的蔡司LSM700显微镜更换为配置优化、功能齐全的 将向JJPVAMC调查人员提供的共焦/多光子显微镜系统。我们 建议用蔡司LSM 980共焦/多光子倒置激光扫描系统取代LSM 700系统 配备AiryScan探测功能的显微镜，可实现3D超分辨率成像。LSM 980共焦/多光子 系统将放置在多用户环境中。将提供技术支持和用户培训，以 支持所有可能受益于高端成像的退伍军人管理局研究项目。购买一架LSM 980 共焦/多光子系统将为高分辨率激光扫描提供尖端系统 共焦/多光子显微镜和相关的高端光学成像技术。