ShEEP Request for Zeiss Modular Laser Scanning Microscope LSM 980

ShEEP 请求蔡司模块化激光扫描显微镜 LSM 980

• 批准号: 10175791
• 负责人: Gregory A. Elder
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175791
• 关键词: 3-Dimensional; Alzheimer' s Disease; Area; Core Facility; Detection; Disease; Environment; Equipment; Goals; Health; Image; Imaging Techniques; Imaging technology; Lasers; Malignant Neoplasms; Medical center; Mental disorders; Microscope; Microscopy; Performance; Program Development; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Sheep; Spinal cord injury; System; Training; Traumatic Brain Injury; Veterans; confocal imaging; in vivo imaging; multiphoton imaging; multiphoton microscopy; operation; optical imaging; research and development

The Research & Development Program at the James J. Peters VA Medical Center (JJPVAMC) has for many years supported a variety of core equipment items that are available to VA investigators and their collaborators. Among the most utilized core equipment items are a set of microscopes that function as part of a Microscopy Core Facility. Central to the operations of this Microscopy Core is a Zeiss LSM 700 microscope that was purchased 7 years. This microscope has been a workhorse that has provided JJPVAMC researches with high-resolution laser scanning confocal imaging for many years. However, with advances in imaging technology, this microscope is now lacks the sensitivity and important functionalities that are essential for high performance confocal/multiphoton imaging and in vivo imaging applications. The goal of this application is to replace an outdated Zeiss LSM700 microscope with an optimally configured and fully functional confocal/multiphoton microscope system that will be be made available to JJPVAMC investigators. We propose to replace the LSM 700 system with a Zeiss LSM 980 confocal/multiphoton inverted laser-scanning microscope with Airyscan detection for 3D super-resolution imaging. The LSM 980 confocal/multiphoton system will be placed in a multiuser environment. Technical support as well user training will be provided to support all VA research projects that might benefit from high-end imaging. Acquisition of an LSM 980 confocal/multiphoton system will provide a cutting-edge system for high resolution laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques.

James J. Peters VA Medical Center（JJPVAMC）的研究与开发计划 多年来支持各种核心设备项目，可供退伍军人事务部调查人员及其 合作者使用最多的核心设备之一是一套显微镜，其功能是 显微镜核心设施。显微镜核心的核心操作是蔡司LSM 700显微镜 买了7年。这台显微镜一直是JJPVAMC研究的主力 高分辨率激光扫描共焦成像技术已经有很多年了。然而，随着成像技术的进步 技术，这种显微镜现在缺乏灵敏度和重要的功能，这是必不可少的高 高性能共焦/多光子成像和体内成像应用。此应用程序的目标是 将过时的蔡司LSM 700显微镜替换为配置最佳且功能齐全的 共聚焦/多光子显微镜系统，将提供给JJPVAMC研究人员。我们 建议用蔡司LSM 980共焦/多光子倒置激光扫描系统取代LSM 700系统 显微镜与Airyscan检测3D超分辨率成像。LSM 980共焦/多光子 系统将被放置在多用户环境中。将提供技术支持和用户培训， 支持所有可能受益于高端成像的VA研究项目。购买LSM 980 共焦/多光子系统将为高分辨率激光扫描提供一种尖端系统 共焦/多光子显微镜和相关的高端光学成像技术。