Core 2: Histopathology and Biospecimen Core

项目摘要/摘要-组织病理学和生物质学核心 组织病理学和生物学家核心(HBC)是弗雷德·哈钦森病的一个重要组成部分 癌症研究中心(FHCRC)肺癌孢子，支持项目的翻译目标 以及未来由孢子资助的发展项目。HBC的使命是促进发现和快速 将发现转化为临床护理。为此，HBC管理获取、注释、 病人来源和孢子资助的实验小鼠的生物菌种的加工和分布 模特们。HBC与西北生物信托公司(NWBioTrust)和华盛顿大学癌症研究中心弗雷德·哈钦森(Fred Hutchinson)合作 财团(CCSG)-一个资助的协作资源，用于获得高质量、注释良好的 用于创新研究的适当同意和不确定的生物谱系。除了生物库 活动，HBC负责管理下游基于组织的分析的性能， 利用弗雷德·哈钦森支持的一套尖端组织病理学和成像能力 癌症研究中心(FHCRC)在其广泛的共享资源网络中。这些功能包括： 但不限于，常规组织病理学服务、病理复查和会诊、免疫组织化学 (IHC)，支链原位杂交(ISH)，DNA/RNA提取，基于纳米串的转录图谱， 多重免疫组织化学(MIHC)和数字图像分析。HBC将提供孢子调查员 使用生物标记物(例如血清)、生物标记物的衍生物(例如未染色的玻片、DNA、RNA)以及质量- 受控数据(例如图像和基于图像分析的数据)。

Project Summary/Abstract – Histopathology and Biospecimen Core The Histopathology and Biospecimen Core (HBC) represents an integral component of the Fred Hutchinson Cancer Research Center (FHCRC) lung cancer SPORE, supporting the translational goals of the Projects as well as future SPORE-funded development projects. The mission of the HBC is to facilitate discovery and rapid translation of discoveries into clinical care. To this end, the HBC manages the acquisition, annotation, processing and distribution of biospecimens from patient-derived and SPORE-funded experimental mouse models. The HBC partners with NWBioTrust (NWBT), and Fred Hutchinson/University of Washington Cancer Consortium (CCSG) – a funded collaborative resource for obtaining high-quality, well-annotated and appropriately-consented and de-identified biospecimens for innovative research. In addition to biobanking activities, the HBC is responsible the for managing the performance of downstream tissue-based assays, leveraging a suite of cutting-edge histopathologic and imaging capabilities supported by the Fred Hutchinson Cancer Research Center (FHCRC) in its extensive network of Shared Resources. These capabilities include, but are not limited to, routine histopathologic services, pathology review and consultation, immunohistochemistry (IHC), branch-chain in-situ hybridization (ISH), DNA/RNA extraction, Nanostring-based transcriptional profiling, multiplex immunohistochemistry (mIHC) and digital image analysis. The HBC will provide SPORE investigators with biospecimens (e.g. serum), derivatives of biospecimens (e.g. unstained slides, DNA,RNA) as well as quality- controlled data (e.g. images and image analysis-based data).