Biospecimen and Pathology Core

生物样本和病理学核心

• 批准号: 10044051
• 负责人: ROBERT H PIERCE
• 金额: $ 36.6万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10044051
• 关键词: Address; African American; Agreement; Alaska Native; Biological Specimen Banks; Clinical; Clinical Data; Collaborations; Collection; Colorectal Cancer; DNA; Data; Data Element; Development; Diagnosis; Ensure; Epidemiology; Fostering; Funding; Future; Genomics; Hispanics; Immunohistochemistry; Incidence; Infrastructure; Institution; Institutional Review Boards; Laboratories; Latino; Lead; Not Hispanic or Latino; Outcome; Pathologist; Pathology; Patients; Pilot Projects; Population; Population Heterogeneity; Positioning Attribute; Preparation H; Process; Program Research Project Grants; Proteomics; Public Health; RNA; Redwood; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; Scientist; Slide; Specimen; Stains; Standardization; System; Technology; Time; Tissue Extracts; Tissue Microarray; Tissue Sample; Training; Translational Research; Tumor Tissue; Work; cancer health disparity; colon cancer patients; community based participatory research; data harmonization; data management; data warehouse; design; digital imaging; ethnic diversity; inclusion criteria; infrastructure development; laboratory equipment; laser capture microdissection; mortality; new technology; programs; racial and ethnic; racial diversity; repository; sample collection; sequencing platform; single-cell RNA sequencing; translational research program; tumor; tumor DNA; user-friendly

Project Summary/Abstract of the Biospecimen and Pathology Core The Biospecimen and Pathology Core (BPC) will provide key infrastructure support for the two Full Research Projects and the Developmental Research Program (DRP), as well as allow for infrastructure development for a subsequent SPORE application. This Core will bring together tumor specimens from colorectal cancer (CRC) cases with diverse racial/ethnic backgrounds; provide a standardized system for specimen collection, tracking, processing, storage, and distribution; provide access to state-of-the-art laboratory technologies; and ensure that the research will be conducted using a community-based participatory research framework. Additionally, this core will build a new, highly annotated biospecimen repository that includes tumor tissue microarrays and tumor DNA and RNA from racially/ethnically diverse CRC patients available for future studies and collaborations. These activities will be achieved through completing the following specific aims: 1. Biospecimen Collection: We will bring together high quality biospecimens from well-characterized racially and ethnically diverse CRC cases. Specifically, we will collect tumor tissue from 840 CRC cases from the following four racial/ethnic populations with vastly different incidence and mortality rates: African Americans, Alaska Natives, Hispanics/Latinos, and non-Hispanic whites. In addition to these key resources, we have access to a number of different biospecimen collections from diverse populations for DRP and future SPORE Projects. 2. Biospecimen Processing: We will process, evaluate, store, and track all biospecimens from the 840 CRC cases using LabmatrixTM, an established, user-friendly laboratory tracking system. These specimens will undergo a detailed centralized pathology review and will be used to extract tumor RNA and DNA. 3. Establish Resources for Future Activities: We will create a new biospecimen repository accompanied by detailed clinical annotation using the tumor samples assembled for this project. It will consist of tumor tissue microarrays and extracted tumor RNA and DNA. 4. Data Management: We will acquire and harmonize demographic, clinical, epidemiologic, and outcomes data associated with the biospecimens to maximize their value. 5. Expanding Capacities for Future Research: We will provide access to state-of-the-art technologies available through the integration of our Core with existing Shared Resources. This will allow us to address the needs of DRP Projects and future Full SPORE Research Projects. In summary, this core will provide critical support to both of the Full Projects, is well-positioned to provide support for Developmental Projects, and will develop a critical data and biospecimen repository for future internal and external collaborations aimed at advancing CRC disparities research.

生物标本和病理学核心项目摘要/摘要 生物标本和病理学核心（BPC）将为两个完整的研究项目提供关键的基础设施支持。 项目和发展研究计划（DRP），以及允许基础设施的发展， 后续的SPORE应用程序。该核心将汇集结直肠癌（CRC）的肿瘤标本 具有不同种族/民族背景的病例;提供样本采集，跟踪， 处理、储存和分配;提供最先进的实验室技术;并确保 这项研究将采用基于社区的参与性研究框架进行。此外，本发明还 这个核心将建立一个新的，高度注释的生物标本库，包括肿瘤组织微阵列， 来自不同种族/人种CRC患者的肿瘤DNA和RNA可用于未来研究， 合作。这些活动将通过完成以下具体目标来实现：1.生物样本 收集：我们将收集高质量的生物标本， 不同的CRC案例。具体而言，我们将从以下四个方面收集840例CRC病例的肿瘤组织 发病率和死亡率差异很大的种族/民族人群：非裔美国人，阿拉斯加土著人， 西班牙裔/拉丁裔和非西班牙裔白人。除了这些关键资源，我们还可以获得一些 为DRP和未来的SPORE项目收集来自不同种群的不同生物标本。2. 生物标本处理：我们将处理、评估、储存和跟踪840 CRC的所有生物标本 使用LabmatrixTM，一个成熟的，用户友好的实验室跟踪系统。这些标本将 进行详细的集中病理学审查，并将用于提取肿瘤RNA和DNA。3.建立 未来活动的资源：我们将创建一个新的生物标本库，并附有详细的临床资料。 使用为该项目组装的肿瘤样本进行注释。它将包括肿瘤组织微阵列， 提取肿瘤RNA和DNA。4.数据管理：我们将收集和协调人口统计学、临床、 与生物标本相关的流行病学和结果数据，以最大限度地发挥其价值。5.扩大 未来的研究能力：我们将通过提供最先进的技术， 整合我们的核心与现有的共享资源。这将使我们能够满足DRP的需求 项目和未来的全孢子研究项目。总之，这一核心将为以下两个方面提供关键支持： 全面项目，是很好的定位，以提供支持的发展项目，并将制定一个关键的 数据和生物标本储存库，用于未来旨在推进CRC的内部和外部合作 差距研究。