Transcriptomics and Repertoire Profiling

核心C：项目总结 这个核心的主要目标是建立和提供强大的实验程序， 转录组和组织驻留淋巴细胞的免疫库。我们将采用创新的方法， 整体转录组和免疫谱分析，表观基因组学和单细胞分析。 对于低输入，群体水平的RNA-Seq，我们将使用我们最近建立的高灵敏度mRNA 捕获和库构建。我们还将提供批量ATAC-Seq用于测量开放染色质。对于单身- 细胞RNA-Seq，我们将部署我们的微流体系统进行大规模单细胞捕获和cDNA条形码编码， 以及用于全转录组扩增的高效板基方法。最后，为了解剖 克隆结构的组织驻留T和B细胞，我们将提供尖端的方法，T细胞受体和 抗体谱分析。

CORE C: PROJECT SUMMARY The main goal of this core is to establish and provide robust experimental procedures for profiling the transcriptomes and immune repertoires of tissue-resident lymphocytes. We will employ innovative methods for whole transcriptome and immune repertoire profiling in bulk populations, epigenomics, and single cell analysis. For low-input, population-level RNA-Seq we will use our recently established protocol for high-sensitivity mRNA capture and library construction. We will also provide bulk ATAC-Seq for measuring open chromatin. For single- cell RNA-Seq, we will deploy our microfluidic system for large-scale single cell capture and cDNA barcoding as well as a highly efficient plate-based method for whole transcriptome amplification. Finally, for dissecting the clonal architecture of tissue-resident T and B cells, we will provide cutting-edge methods for T cell receptor and antibody repertoire profiling.