Transcriptomics and Repertoire Profiling

核心C：项目总结 这个核心的主要目标是建立和提供健壮的实验程序来分析 组织驻留淋巴细胞的转录本和免疫谱系。我们将采用创新的方法来 整体转录组和免疫谱分析、表观基因组学和单细胞分析。 对于低输入、种群水平的RNA-Seq，我们将使用我们最近建立的高敏感性mRNA的检测方法 抓捕和图书馆建设。我们还将提供用于测量开放染色质的散装ATAC-Seq。对于单人- Cell RNA-Seq，我们将部署我们的微流控系统，用于大规模的单细胞捕获和cDNA条形码 以及一种用于整个转录组扩增的高效平板方法。最后，为了解剖 组织驻留T和B细胞的克隆结构，我们将为T细胞受体和 抗体谱系分析。

CORE C: PROJECT SUMMARY The main goal of this core is to establish and provide robust experimental procedures for profiling the transcriptomes and immune repertoires of tissue-resident lymphocytes. We will employ innovative methods for whole transcriptome and immune repertoire profiling in bulk populations, epigenomics, and single cell analysis. For low-input, population-level RNA-Seq we will use our recently established protocol for high-sensitivity mRNA capture and library construction. We will also provide bulk ATAC-Seq for measuring open chromatin. For single- cell RNA-Seq, we will deploy our microfluidic system for large-scale single cell capture and cDNA barcoding as well as a highly efficient plate-based method for whole transcriptome amplification. Finally, for dissecting the clonal architecture of tissue-resident T and B cells, we will provide cutting-edge methods for T cell receptor and antibody repertoire profiling.