Advanced Single Cell Technology Innovation Core
先进单细胞技术创新核心
基本信息
- 批准号:10729389
- 负责人:
- 金额:$ 14.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-19 至 2028-08-31
- 项目状态:未结题
- 来源:
- 关键词:ATAC-seqAddressApoptosisBar CodesCRISPR interferenceCancer CenterCell CommunicationCell DeathCell SurvivalCell modelCellsChromatinClustered Regularly Interspaced Short Palindromic RepeatsComputer ModelsCytometryEpitopesGene ExpressionGene Expression ProfileGene Expression ProfilingGene PoolGenomic LibraryGenomicsGoalsGuide RNAHumanImageImmunofluorescence ImmunologicIn SituIndividualJointsLibrariesLinkMalignant NeoplasmsMessenger RNAMethodsMicrofluidicsMolecularMolecular AnalysisMolecular TargetMusPeriodicityPharmaceutical PreparationsPreparationProteinsProteomicsProtocols documentationQuality ControlRNAResearch PersonnelResolutionSamplingSliceSolid NeoplasmSystemTechnologyTherapeuticTissuesTranscriptValidationanalytical methodcell typecellular imagingdroplet sequencingexperimental analysislive cell imagingmicrofluidic technologymultiple omicsposttranscriptionalresponsesingle cell analysissingle cell technologysingle-cell RNA sequencingtechnological innovationtooltranscriptome sequencingtumortumor microenvironment
项目摘要
PROJECT SUMMARY
The proposed Cancer Systems Therapeutics (CaST) Center focuses on understanding and targeting molecularly
distinct yet co-existing cell states and transitions that establish and maintain tumors and tumor
microenvironments at a systems level. These goals demand analytical methods with single-cell resolution. The
Advanced Single Cell Technology Innovation Core (ASCeTIC) will provide state-of-the-art technology for single-
cell genomic analysis, multiplexed single-cell molecular perturbation, and in situ validation to CaST investigators.
We will offer a suite of tools including multi-omic methods for joint single-cell RNA/ATAC-seq, single-cell
imaging/RNA-seq (SCOPE-seq), and single-cell CRISPRi/RNA-seq (CROP-seq). Recognizing the importance
of spatial profiling for both in situ validation and studies of cell-cell interactions in the tumor microenvironment,
we will also offer immunohistochemical analysis, RNA-FISH, and cyclic immunofluorescence (cycIF), which can
simultaneously quantify tens of protein epitopes in individual cells in a single tissue section.
项目总结
拟议的癌症系统治疗(CAST)中心专注于了解和靶向分子
建立和维持肿瘤和肿瘤的不同但共存的细胞状态和转变
系统层面的微环境。这些目标需要具有单细胞分辨率的分析方法。这个
先进的单电池技术创新核心(ASSITIC)将为单电池提供最先进的技术
细胞基因组分析,多重单细胞分子扰动,以及对CAST研究人员的现场验证。
我们将提供一套工具,包括用于联合单细胞RNA/atac-seq、单细胞的多组学方法。
成像/RNA-SEQ(SCOPE-SEQ)和单细胞CRISPRI/RNA-SEQ(CROP-SEQ)。认识到
用于肿瘤微环境中细胞-细胞相互作用的原位验证和研究的空间分布,
我们还将提供免疫组织化学分析、RNA-FISH和循环免疫荧光(CycIF),这可以
同时量化单个组织切片中单个细胞中的数十个蛋白质表位。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Peter Alan Sims其他文献
Peter Alan Sims的其他文献
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{{ truncateString('Peter Alan Sims', 18)}}的其他基金
The Bioinformatics and Single Cell Analysis Core
生物信息学和单细胞分析核心
- 批准号:
10443136 - 财政年份:2022
- 资助金额:
$ 14.25万 - 项目类别:
The Bioinformatics and Single Cell Analysis Core
生物信息学和单细胞分析核心
- 批准号:
10612961 - 财政年份:2022
- 资助金额:
$ 14.25万 - 项目类别:
Large-Scale Integration of Single Cell RNA-Seq and High-Content Imaging for Analyzing Drug Response in Cancer
大规模整合单细胞 RNA 测序和高内涵成像来分析癌症药物反应
- 批准号:
9035846 - 财政年份:2016
- 资助金额:
$ 14.25万 - 项目类别:
A Microfluidic System for Image-Guided RNA-Seq of Single Cells in Glioblastoma
用于胶质母细胞瘤单细胞图像引导 RNA 测序的微流体系统
- 批准号:
9129717 - 财政年份:2012
- 资助金额:
$ 14.25万 - 项目类别:
A Microfluidic System for Image-Guided RNA-Seq of Single Cells in Glioblastoma
用于胶质母细胞瘤单细胞图像引导 RNA 测序的微流体系统
- 批准号:
8424800 - 财政年份:2012
- 资助金额:
$ 14.25万 - 项目类别:
Multiplexed, Single Molecule Protein Identification for Single Cell Proteomics
单细胞蛋白质组学的多重、单分子蛋白质鉴定
- 批准号:
8549924 - 财政年份:2012
- 资助金额:
$ 14.25万 - 项目类别:
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