Single Cell Core
Single Cell Core
批准号:
10419868
负责人:
Peter Alan Sims
金额:
$29.29万
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
未结题
起止时间:
2017-01-01 至 2027-02-28
关键词:
2019-nCoVActivities of Daily LivingAddressAdultAgeAntibodiesAntigensB-Cell Antigen ReceptorBar CodesBlood CirculationCell CountCellsCytomegalovirusDNAEpitopesFlow CytometryFundingGenomic LibraryGenomicsHumanImmuneImmune responseImmunologic ReceptorsImmunologyImmunophenotypingIndividualInfantInfluenzaJointsLibrariesLinkMapsMembrane ProteinsMessenger RNAMicrofluidicsOrgan DonorPhenotypePopulationPreparationProteomeProtocols documentationQuality ControlReportingResearch PersonnelResolutionResourcesRestSARS-CoV-2 antigenSamplingSiteSorting - Cell MovementSpecificityStainsSurfaceT cell receptor repertoire sequencingT-Cell ReceptorT-LymphocyteTechnologyTissue DonorsTissue SampleTissuesVaccinesViralVirusantigen-specific T cellscomparativecost effectiveexperimental studyindexingmultiple omicsprotein expressionprotein profilingsingle cell analysissingle cell technologysingle-cell RNA sequencingtooltranscriptome
中文摘要
单电池芯:项目总结
单细胞核心将为单细胞多组学分析提供最先进的实验技术。两
用于免疫表型分析的常规scRNA-seq的主要局限性包括缺乏与免疫表型之间的相关性。
mRNA水平和表面蛋白表达以及缺乏来自免疫受体序列的克隆同一性。
核心将提供同时分析mRNA、免疫受体序列、
和单个细胞的表面蛋白表达。对于细胞数量有限的实验,我们将
还提供scPLATE-seq,一个用于索引排序和scRNA-seq的全自动化平台,
细胞数量少。鉴于重点是纵向和横向分析病毒特异性免疫反应,
组织,将免疫表型如表面蛋白表达,转录组,
具有抗原特异性的TCR序列也是高度期望的。这些信息将使我们能够确定
在病毒特异性T细胞群体中每个细胞的表型亚群和克隆身份,以锚病毒特异性T
从不同的个体,年龄和组织部位的细胞到我们的参考图谱,并研究其功能
单细胞分辨率的容量。核心将提供可扩展的技术,
和T细胞受体序列与单个细胞的靶向mRNA和表面蛋白质谱(TetTCR-1)。
seqHD)。我们将为广泛的病毒和疫苗开发和优化条形码四聚体池
包括CMV、流感病毒和SARS-CoV-2进行联合分析,T细胞受体抗原识别,克隆分型,
和免疫表型。
英文摘要
SINGLE CELL CORE: PROJECT SUMMARY
The Single Cell Core will provide state-of-the-art experimental technology for single-cell multi-omic profiling. Two
major limitations of conventional scRNA-seq for immunophenotyping include the lack of correlation between
mRNA levels and surface protein expression and the lack of clonal identity from immune receptor sequence.
The Core will provide access to technologies for simultaneous profiling of mRNA, immune receptor sequence,
and surface protein expression from individual cells at scale. For experiments with limited cell numbers, we will
also provide scPLATE-seq, a fully automated platform for index sorting and scRNA-seq that is cost-effective for
low cell numbers. Given the focus on analyzing virus-specific immune responses longitudinally and across
tissues, the ability to associate immunophenotypes such as surface protein expression, the transcriptome, and
TCR sequence with antigen specificity is also highly desirable. This information will allow us to determine the
phenotypic subset and clonal identity of each cell in a virus-specific T cell population, to anchor virus-specific T
cells from different individuals, ages, and tissue sites to our reference map, and to investigate their functional
capacities with single-cell resolution. The Core will provide scalable technology for associating antigen specificity
and T cell receptor sequence with targeted mRNA and surface protein profiles of individual cells (TetTCR-
seqHD). We will develop and optimize barcoded tetramer pools for a broad range of viruses and vaccines
including CMV, influenza, and SARS-CoV-2 for joint analysis T cell receptor antigen recognition, clonotype,
and immunophenotype in individual T cells.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Advanced Single Cell Technology Innovation Core
-
批准号:10729389
-
项目类别:
-
资助金额:$14.25万
-
财政年份:2023
-
负责人:Peter Alan Sims
-
依托单位:
The Bioinformatics and Single Cell Analysis Core
-
批准号:10443136
-
项目类别:
-
资助金额:$16.94万
-
财政年份:2022
-
负责人:Peter Alan Sims
-
依托单位:
The Bioinformatics and Single Cell Analysis Core
-
批准号:10612961
-
项目类别:
-
资助金额:$17.0万
-
财政年份:2022
-
负责人:Peter Alan Sims
-
依托单位:
Single Cell Core
-
批准号:10594524
-
项目类别:
-
资助金额:$37.45万
-
财政年份:2017
-
负责人:Peter Alan Sims
-
依托单位:
Large-Scale Integration of Single Cell RNA-Seq and High-Content Imaging for Analyzing Drug Response in Cancer
-
批准号:9035846
-
项目类别:
-
资助金额:$31.61万
-
财政年份:2016
-
负责人:Peter Alan Sims
-
依托单位:
Transcriptomics and Repertoire Profiling
-
批准号:10176370
-
项目类别:
-
资助金额:$48.18万
-
财政年份:2013
-
负责人:Peter Alan Sims
-
依托单位:
Transcriptomics and Repertoire Profiling
-
批准号:10426134
-
项目类别:
-
资助金额:$47.4万
-
财政年份:2013
-
负责人:Peter Alan Sims
-
依托单位:
A Microfluidic System for Image-Guided RNA-Seq of Single Cells in Glioblastoma
-
批准号:9129717
-
项目类别:
-
资助金额:$17.28万
-
财政年份:2012
-
负责人:Peter Alan Sims
-
依托单位:
A Microfluidic System for Image-Guided RNA-Seq of Single Cells in Glioblastoma
-
批准号:8424800
-
项目类别:
-
资助金额:$17.22万
-
财政年份:2012
-
负责人:Peter Alan Sims
-
依托单位:
Multiplexed, Single Molecule Protein Identification for Single Cell Proteomics
-
批准号:8549924
-
项目类别:
-
资助金额:$19.4万
-
财政年份:2012
-
负责人:Peter Alan Sims
-
依托单位:
Multiplexed, Single Molecule Protein Identification for Single Cell Proteomics
-
批准号:8413642
-
项目类别:
-
资助金额:$21.9万
-
财政年份:2012
-
负责人:Peter Alan Sims
-
依托单位:
A Microfluidic System for Image-Guided RNA-Seq of Single Cells in Glioblastoma
-
批准号:8549246
-
项目类别:
-
资助金额:$17.28万
-
财政年份:2012
-
负责人:Peter Alan Sims
-
依托单位:
A High-Throughput Molecular Profiling Core for the CaST Center
-
批准号:9976482
-
项目类别:
-
资助金额:$24.23万
-
财政年份:--
-
负责人:Peter Alan Sims
-
依托单位:
A High-Throughput Molecular Profiling Core for the CaST Center
-
批准号:9752994
-
项目类别:
-
资助金额:$23.59万
-
财政年份:--
-
负责人:Peter Alan Sims
-
依托单位:
A High-Throughput Molecular Profiling Core for the CaST Center
-
批准号:10021246
-
项目类别:
-
资助金额:$1.14万
-
财政年份:--
-
负责人:Peter Alan Sims
-
依托单位:
海外基金