Animal and Microsurgery Core (Core B)

核心摘要 动物和显微外科核心（核心B）的目的是建立一个重点设施， 两个项目中的每一个项目的研究人员都有足够的特定基因型动物繁殖， 标准化的心脏或皮肤移植和用于所提出的实验的耐受诱导程序。 核心B将提供关键服务，使主要研究者及其工作人员能够专注于 实验和科学的努力。集中繁殖和筛选后代将确保动物 所需的基因型可用于实验。由集中来源进行的移植将 标准化程序，最大限度地减少两个项目所用移植物的手术差异， 允许为项目1（研究新的T细胞机制在强大的耐受性）产生一些长期受体 用于项目2（研究公差的保持），以尽量减少实验成本。此外，本发明还提供了一种方法， 采购物资，如缝合线，麻醉剂和免疫抑制剂将在批量完成， 项目，以降低成本。核心B的具体任务是：a）对用于以下目的的动物进行繁殖和基因分型 两个项目; B）按照项目负责人及其工作人员的指示进行心脏移植; c）准备 并注射供体脾细胞和施用指定的免疫抑制疗法; d）监测 移植的心脏，移植受体的健康状况，以及排异反应的记录时间; e）牺牲 按照主要研究者及其工作人员的指示进行移植动物，并收集器官用于 组织学，并分发给其他项目人员进行免疫表型和功能分析 细胞

CORE SUMMARY The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides investigators in each of the two projects with sufficient breeding of animals with specified genotypes and with standardized cardiac or skin transplantation and tolerance induction procedures for the proposed experiments. Core B will provide a key service role that will enable the Principal Investigators and their staff to focus on experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals of required genotypes are available for experiments. Transplantation performed by a centralized source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and allow some long-term acceptors generated for Project 1 (studying novel T cell mechanisms in robust tolerance) to be used for Project 2 (studying the maintenance of tolerance) to minimize experimental costs. In addition, the purchase of supplies, such as sutures, anesthetics and immunosuppressants will be done in bulk for both projects to reduce cost. The specific tasks of Core B will be to: a) breed and genotype animals to be used for both projects; b) transplant heart allografts as directed by the project leaders and their personnel; c) prepare and inject donor splenocytes and administer indicated immunosuppressive therapies; d) monitor the beating of the transplanted hearts, the health of the transplant recipients, and record times of rejection; e) sacrifice transplanted animals as directed by the Principal Investigators and their personnel, and collect organs for histology, as well as to distribute to other project personnel for phenotypic and functional analysis of immune cells.