Animal and Microsurgery Core (Core B)

核心摘要 动物和显微外科核心(核心B)的目的是建立一个重点设施，提供 两个项目中每个项目的调查人员都有足够的动物繁殖指定的基因类型和 为拟议的实验标准化心脏或皮肤移植和耐受诱导程序。 核心B将提供一个关键的服务角色，使首席调查员及其工作人员能够专注于 实验和科学方面的努力。对后代的集中繁育和筛选将确保动物 所需的基因类型可用于实验。由集中来源进行的移植将 标准化程序，将两个项目使用的移植物之间的手术造成的差异降至最低 允许为项目1生成一些长期受体(研究强健耐受中的新T细胞机制) 用于项目2(研究公差的维持)，以最大限度地减少实验成本。此外, 购买缝合线、麻醉剂和免疫抑制剂等用品将批量进行。 降低成本的项目。核心B的具体任务将是：A)饲养和为用于 两个项目；b)在项目领导及其人员的指导下移植同种异体心脏；c)准备 和注射捐赠者的脾细胞并实施指定的免疫抑制治疗；d)监测 移植的心脏，移植受者的健康，以及创纪录的排斥次数；e)牺牲 按照首席调查员及其工作人员的指示移植动物，并收集器官用于 组织学，以及分发给其他项目人员进行免疫表型和功能分析 细胞。

CORE SUMMARY The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides investigators in each of the two projects with sufficient breeding of animals with specified genotypes and with standardized cardiac or skin transplantation and tolerance induction procedures for the proposed experiments. Core B will provide a key service role that will enable the Principal Investigators and their staff to focus on experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals of required genotypes are available for experiments. Transplantation performed by a centralized source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and allow some long-term acceptors generated for Project 1 (studying novel T cell mechanisms in robust tolerance) to be used for Project 2 (studying the maintenance of tolerance) to minimize experimental costs. In addition, the purchase of supplies, such as sutures, anesthetics and immunosuppressants will be done in bulk for both projects to reduce cost. The specific tasks of Core B will be to: a) breed and genotype animals to be used for both projects; b) transplant heart allografts as directed by the project leaders and their personnel; c) prepare and inject donor splenocytes and administer indicated immunosuppressive therapies; d) monitor the beating of the transplanted hearts, the health of the transplant recipients, and record times of rejection; e) sacrifice transplanted animals as directed by the Principal Investigators and their personnel, and collect organs for histology, as well as to distribute to other project personnel for phenotypic and functional analysis of immune cells.