Animal and Microsurgery Core (Core B)

项目总结/摘要 动物和显微外科核心（核心B）的目的是建立一个重点设施， 两个项目中的每一个项目的研究人员都有足够的特定基因型动物育种， 标准化的心脏或皮肤移植和用于所提出的实验的耐受诱导程序。 核心B将提供关键服务，使主要研究者及其工作人员能够专注于 实验和科学的努力。集中繁殖和筛选后代将确保动物 所需的基因型可用于拟议的实验。移植由一个集中的 来源将标准化的程序，尽量减少手术强加的差异，在移植使用的两个 项目，并允许为项目1产生一些长期接受者（解决 维持移植耐受性）用于项目2（面对稳定的 由记忆T细胞引起的移植耐受性）以最小化实验成本。此外，购买 供应，如缝合线，麻醉剂和免疫抑制剂将大量完成这两个项目，以减少 成本核心B的具体任务是：a）对两个项目使用的动物进行繁殖和基因分型; B） 在项目负责人及其工作人员的指导下进行同种异体心脏移植; c）准备和注射供体 d）监测移植的脾细胞的搏动; 心脏，移植受体的健康，以及排斥反应的记录时间; e）将移植的动物处死， 在主要研究者及其工作人员的指导下，收集器官进行组织学检查，并分发 用于免疫细胞的表型和功能分析。

Project Summary/Abstract The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides investigators in each of the two projects with sufficient breeding of animals with specified genotypes, and with standardized cardiac or skin transplantation and tolerance induction procedures for the proposed experiments. Core B will provide a key service role that will enable the Principal Investigators and their staff to focus on experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals of required genotypes are available for the proposed experiments. Transplantation performed by a centralized source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and allow some long-term acceptors generated for Project 1 (addressing vulnerabilities to the maintenance of transplantation tolerance) to be used for Project 2 (confronting the barrier to stable transplantation tolerance posed by memory T cells) to minimize experimental costs. In addition, the purchase of supplies, such as sutures, anesthetics and immunosuppressants will be done in bulk for both projects to reduce cost. The specific tasks of Core B will be to: a) breed and genotype animals to be used for both projects; b) transplant heart allografts as directed by the project leaders and their personnel; c) prepare and inject donor splenocytes and administer indicated immunosuppressive therapies; d) monitor the beating of the transplanted hearts, the health of the transplant recipients, and record times of rejection; e) sacrifice transplanted animals as directed by the Principal Investigators and their personnel, and collect organs for histology, as well as to distribute to other project personnel for phenotypic and functional analyses of immune cells.