Leica Stellaris 8 Confocal Microscope

Leica Stellaris 8 共焦显微镜

• 批准号: 10176769
• 负责人: CHRISTIAN I HONG
• 金额: $ 59.81万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176769
• 关键词: 3-Dimensional; Address; Aging; Biomedical Research; Cells; Collection; Communities; Confocal Microscopy; Contract Services; Data Set; Ensure; Financial Support; Funding; Grant; Human Resources; Image; Image Enhancement; Laboratories; Maintenance; Medicine; Microscope; Microscopy; Organoids; Process; Productivity; Proteins; Publications; Request for Applications; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Scientist; Secure; Services; Speed; Stains; Structure; System; Technology; Time; Tissues; United States National Institutes of Health; Universities; college; cost; experience; experimental study; fluorescence imaging; imaging capabilities; imaging facilities; improved; instrument; instrumentation; interest

PROJECT SUMMARY/ABSTRACT This proposal requests funding for a Leica Stellaris 8 confocal microscope system to be housed and used in the University of Cincinnati (UC) Live Microscopy Core (LMC). The scientists at UC College of Medicine (CoM) have greatly benefited from the LMC providing imaging services that have propelled biomedical research in the CoM. In the past two years, the LMC served 56 laboratories funded by 35 NIH grants, which resulted in more than 25 publications. The productivity and efficiency of the LMC, however, have been gradually diminished over the past three years due to: 1) the aging of our confocal microscopes and 2) lack of technical advancements of imaging capabilities. Currently, the LMC operates two confocal microscopes, Zeiss LSM510 NLO and Zeiss LSM710 (purchased in 2004 and 2010, respectively), both of which have experienced increasing maintenance issues and downtime, resulting in substantial inconveniences and delays in numerous research projects. Additionally, these older instruments have technical limitations in speed, sensitivity, resolution, and versatility. The NIH-funded investigators at UC have a strong need for advanced live imaging capabilities, and require instrumentation to: 1) perform long time lapse fluorescence imaging experiments in live cells, tissues, and 3D organoid cultures, 2) collect 3D fluorescence images with sufficient resolution to localize proteins and structures of interest in stained cells, tissues, and organoids, and 3) simultaneously detect numerous fluorescent markers with efficient spectral separation. To mitigate this critical need, this application requests funds to acquire an advanced confocal microscope system, the Leica Stellaris 8, to supplement the existing microscopes in the LMC. This new instrument will serve two major purposes: add capacity to our currently over-utilized confocal systems, and enable new imaging experiments that cannot be achieved with the existing technology. Specifically, this new microscope will greatly improve the viability of samples for long time lapse imaging, enhance resolution and sensitivity to detect small subcellular details, and improve imaging speed for efficient collection of large 3D data sets and capturing fast dynamic processes in live samples. The Leica Stellaris 8 system will be installed within the current central imaging facility and will be operated by the facility technician with 15 years of experience in all aspects of confocal microscopy experimentation and analysis. This application is supported by substantial interest, need, and financial support at the University, and will serve a large NIH-funded scientific community. We have secured funding guarantees from the CoM and UC Office of Research to ensure the instrument is covered under service contract at all times, and a financial plan with backup departmental funds for ongoing operational and personnel costs to operate the microscope on a day-to-day basis. The requested instrument is ideally suited to address both the technical specifications and capacity issues for the 8 NIH-funded Major Users on this grant.

项目总结/摘要 该提案要求为Leica Stellaris 8共聚焦显微镜系统提供资金，该系统将在 辛辛那提大学（UC）实时显微镜核心（LMC）。加州大学医学院（CoM）的科学家们 极大地受益于LMC提供的成像服务，推动了CoM的生物医学研究。 在过去的两年里，LMC为56个实验室提供服务，这些实验室由35个NIH赠款资助， 出版物。然而，LMC的生产力和效率在过去逐渐下降 三年，由于：1）我们的共焦显微镜老化和2）缺乏成像技术的进步 能力的 目前，LMC操作两个共焦显微镜，蔡司LSM 510 NLO和蔡司LSM 710 （分别于2004年和2010年购买），这两台机器的维护问题越来越多， 停机时间，导致大量的不便和延误，在许多研究项目。此外，这些 较旧的仪器在速度、灵敏度、分辨率和多功能性方面具有技术限制。nih资助的 加州大学的研究人员强烈需要先进的实时成像能力，并需要仪器：1） 在活细胞、组织和3D类器官培养物中进行长时间间隔荧光成像实验，2） 收集具有足够分辨率的3D荧光图像，以在染色的细胞中定位感兴趣的蛋白质和结构。 细胞、组织和类器官，以及3）同时检测具有高效光谱特性的多种荧光标记物。 分居 为了缓解这一关键需求，该申请要求资金购买先进的共聚焦显微镜 Leica Stellaris 8是LMC现有显微镜的补充。这一新工具将有助于 两个主要目的：增加我们目前过度使用的共焦系统的容量，并实现新的成像 这是现有技术无法实现的。具体来说，这种新的显微镜将大大 提高长时间间隔成像的样品的可行性，提高分辨率和灵敏度，以检测小的 亚细胞细节，并提高成像速度，以有效收集大型3D数据集和快速捕获 活样本中的动态过程。Leica Stellaris 8系统将安装在目前的中央 成像设施，并将由在共聚焦成像的各个方面具有15年经验的设施技术人员操作。 显微镜实验和分析。这一申请得到了大量兴趣、需求和 在大学的财政支持，并将服务于一个大型NIH资助的科学界。我们已经获得 来自CoM和UC研究办公室的资金保证，以确保仪器在服务范围内 合同，并制定财务计划，为持续的业务和人员提供备用部门资金 日常操作显微镜的费用。所要求的文书非常适合处理 这两个技术规格和能力的问题，为8个NIH资助的主要用户在此赠款。

项目成果

Inhibition of CSPG receptor PTPσ promotes migration of newly born neuroblasts, axonal sprouting, and recovery from stroke.

• DOI: 10.1016/j.celrep.2022.111137
• 发表时间: 2022-07-26
• 期刊: CELL REPORTS
• 影响因子: 8.8
• 作者: Luo, Fucheng;Wang, Jiapeng;Zhang, Zhen;You, Zhen;Bedolla, Alicia;Okwubido-Williams, Feargod;Huang, L. Frank;Silver, Jerry;Luo, Yu
• 通讯作者: Luo, Yu