ICP-QQQ

ICP-MS/MS

• 批准号: 10177184
• 负责人: Ana Maria Rule
• 金额: $ 34.95万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177184
• 关键词: 14 year old; American Indians; Arsenic; Biomedical Research; Clinical Investigator; Communities; Coupled; Data; Digestion; Discipline; Electronic cigarette; Environmental Engineering technology; Environmental Health; Environmental Pollutants; Equipment; Evaluation; Exposure to; Funding; Glioblastoma; Goals; Grant; Health; Heart Diseases; High Pressure Liquid Chromatography; Immune response; Inductively Coupled Plasma Mass Spectrometry; Infection; Influenza; Magnetism; Malignant Neoplasms; Malignant neoplasm of liver; Medical; Medicine; Metal exposure; Metals; Minor; Pilot Projects; Population Heterogeneity; Prevention; Public Health; Public Health Schools; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Risk Factors; Services; Stroke; Students; System; Technical Expertise; Technology; Toxic effect; Trace metal; Translations; United States National Institutes of Health; Universities; cancer survival; equipment acquisition; experience; hyperthermia treatment; instrument; instrumentation; mass spectrometer; medical schools; microwave electromagnetic radiation; novel therapeutic intervention; particle; rapid growth; school health; tumor

Project Summary/Abstract: The overall goal of this proposal is to acquire funds to purchase a triple quadrupole mass spectrometer and a new microwave digestor to replace a 14-year old Agilent 7500ce ICP-MS and a MARS5 microwave. The funds will be used to purchase an Agilent 8900 ICP-QQQ system that can be coupled with our existing Agilent 1260 HPLC system which will be dedicated to support NIH-funded investigators requiring quantitative metal analysis, including metal speciation. A group of investigators (6 major, 5 minor) has been identified whose research (75.1% NIH-funded) will greatly benefit from analyses performed with the requested instrumentation. The Trace Metals Lab (TML) in the Department of Environmental Health and Engineering at Johns Hopkins Bloomberg School of Public Health houses the only ICP-MS in the medical campus, which includes the School of Medicine (SOM) and the School of Public Health. The TML has been providing accurate, sensitive, and reliable trace metal analysis for health-related research since 2006. This shared resource provides funded investigators with technical expertise and access to large equipment for quantitative analysis of trace metals. The JHU TML is used heavily by NIH-funded grants supporting research that ranges from exposure to metals from e-cigarettes (R01), evaluation of Magnetic Hyperthermia Therapy (MHT) against infiltrating glioblastoma (R01) and liver cancer tumors (R01), assessment of arsenic exposure on immune responses to influenza (R01), Arsenic and other metal exposures in American Indian communities (R01), among others. The research that will benefit from this instrumentation will have enormous impacts on the disciplines of Medicine and Public Health. With ICP- QQQ, HPLC, microwave digestion, and Single Particle analysis capabilities, our lab will continue to advance the translation of basic biomedical research to treatments and cures, and will also contribute to prevention through strategies for reducing exposure to environmental pollutants for diverse populations. The number of basic and clinical investigators requiring analytical services of trace metals has experienced a rapid growth. As a result, usage of the existing ICP-MS instrument is currently at full capacity. The new instrumentation will also benefit studies that are not currently NIH funded, pilot projects to collect data for NIH grants, and student research projects. The ICP-MS capability of the JHU TML will also contribute to the long-range biomedical research goals of the Johns Hopkins University. This instrumentation will benefit research to understand basic cellular toxicity, risk factors for infection, develop important new therapeutic strategies against cancer, and advance our understanding of stroke mechanisms, heart disease, cancer survival, and immune responses. As outlined in this application, acquisition of this equipment will make this technology available to a large number of investigators, continuing with the goals of the Trace Metals Lab, the Department of Environmental Health and Engineering, the Bloomberg School or Public Health, the Johns Hopkins University, and the Biomedical community as a whole.

项目摘要/摘要： 该提案的总体目标是筹集资金购买三重四极杆质谱仪和一台 新型微波消解器取代了使用了 14 年的 Agilent 7500ce ICP-MS 和 MARS5 微波。资金 将用于购买 Agilent 8900 ICP-MS/MS 系统，该系统可与我们现有的 Agilent 1260 结合使用 HPLC 系统将致力于支持 NIH 资助的需要定量金属分析的研究人员， 包括金属形态。已确定一组研究人员（6 名专业，5 名副修），他们的研究 （75.1% 由 NIH 资助）将极大地受益于使用所需仪器进行的分析。踪迹 约翰霍普金斯大学彭博环境健康与工程系金属实验室 (TML) 公共卫生学院拥有医学院内唯一的 ICP-MS （SOM）和公共卫生学院。 TML 一直提供准确、灵敏且可靠的痕量金属 自 2006 年以来对健康相关研究的分析。这一共享资源为受资助的研究人员提供了 技术专业知识和使用大型设备进行痕量金属定量分析的能力。 JHU TML 是 NIH 资助的赠款大量使用，支持从接触电子烟中的金属等研究 (R01)，磁热疗 (MHT) 对浸润性胶质母细胞瘤 (R01) 和肝脏的评估 癌症肿瘤 (R01)、砷暴露对流感免疫反应的评估 (R01)、砷和 美洲印第安人社区 (R01) 等的其他金属暴露。受益的研究 该仪器将对医学和公共卫生学科产生巨大影响。与 ICP- QQQ、HPLC、微波消解和单颗粒分析能力，我们实验室将不断推进 将基础生物医学研究转化为治疗和治愈方法，也将有助于通过预防 减少不同人群接触环境污染物的策略。基本数量和 需要痕量金属分析服务的临床研究人员经历了快速增长。因此， 现有 ICP-MS 仪器的使用目前已满负荷。新仪器也将受益 目前未获得 NIH 资助的研究、为 NIH 资助收集数据的试点项目以及学生研究 项目。 JHU TML 的 ICP-MS 能力也将有助于实现长期生物医学研究目标 约翰霍普金斯大学的。该仪器将有利于了解基本细胞毒性的研究， 感染的危险因素，开发重要的新癌症治疗策略，并推进我们的研究 了解中风机制、心脏病、癌症生存和免疫反应。如本文所述 应用程序，购买该设备将使该技术可供大量研究人员使用， 继续实现微量金属实验室、环境健康与工程系、 布隆伯格学院或公共卫生学院、约翰·霍普金斯大学以及整个生物医学界。

项目成果

Residential proximity to metal emitting industries and toenail metal concentration in the US Gulf States.

美国海湾国家的住宅靠近金属排放工业和趾甲金属集中区。

• DOI: 10.21203/rs.3.rs-3210942/v1
• 发表时间: 2023
• 期刊: Research square
• 作者: Lin,JoyceJy;Werder,Emily;Lawrence,KaitlynG;Jackson2nd,WBraxton;Sandler,DaleP;Dickerson,AishaS;Engel,LawrenceS;Rule,AnaM
• 通讯作者: Rule,AnaM