ICP-QQQ

项目摘要/摘要： 这项提议的总体目标是获得资金，购买一台三重四极杆质谱仪和一台 新的微波消化器，以取代已有14年历史的安捷伦7500ce电感耦合等离子体质谱仪和MARS5微波炉。这些资金 将用于购买安捷伦8900 ICP-QQQ系统，该系统可以与我们现有的安捷伦1260相结合 高效液相系统将致力于支持NIH资助的需要定量金属分析的调查人员， 包括金属形态。已经确定了一组调查人员(6名主要调查人员，5名次要调查人员)，他们的研究 (75.1%由美国国立卫生研究院资助)将从使用所要求的仪器进行的分析中受益匪浅。踪迹 约翰霍普金斯大学彭博社环境卫生与工程系金属实验室 公共卫生学院拥有包括医学院在内的医学校园内唯一的电感耦合等离子体质谱 (SOM)和公共卫生学院。TML一直在提供准确、灵敏和可靠的痕量金属 2006年以来与健康相关的研究分析。这一共享资源为资助的调查人员提供了 技术专长和获得用于痕量金属定量分析的大型设备。JHU TML是 由美国国立卫生研究院资助的赠款大量使用，支持从接触电子烟中的金属到研究 (R01)，磁热疗法(MHT)对浸润性胶质母细胞瘤和肝脏的评价(R01) 癌症肿瘤(R01)，砷暴露对流感免疫反应的评估(R01)，砷和 美洲印第安人社区的其他金属暴露(R01)，以及其他。将会受益的研究 这一仪器将对医学和公共卫生学科产生巨大影响。有了ICP- QQQ、高效液相、微波消解和单粒子分析能力，我们的实验室将继续推进 将基础生物医学研究转化为治疗和治疗，并将通过以下方式促进预防 减少不同人群暴露于环境污染物的战略。基本数量和基本数量 需要痕量金属分析服务的临床研究人员经历了快速增长。结果, 现有的电感耦合等离子体质谱仪器目前已满负荷使用。新的仪器设备也将受益 目前没有得到NIH资助的研究，为NIH拨款收集数据的试点项目，以及学生研究 项目。JHU TML的电感耦合等离子体质谱仪的能力也将为远程生物医学研究目标做出贡献 约翰·霍普金斯大学的。这种仪器将有助于研究了解基本的细胞毒性， 感染的危险因素，开发重要的抗癌新治疗策略，并推动我们的 了解中风机制、心脏病、癌症存活率和免疫反应。如本文件中所述 应用，获得这一设备将使这项技术可供大量调查人员使用， 继续实现痕量金属实验室、环境卫生和工程部、 彭博公共卫生学院、约翰霍普金斯大学和整个生物医学界。

Project Summary/Abstract: The overall goal of this proposal is to acquire funds to purchase a triple quadrupole mass spectrometer and a new microwave digestor to replace a 14-year old Agilent 7500ce ICP-MS and a MARS5 microwave. The funds will be used to purchase an Agilent 8900 ICP-QQQ system that can be coupled with our existing Agilent 1260 HPLC system which will be dedicated to support NIH-funded investigators requiring quantitative metal analysis, including metal speciation. A group of investigators (6 major, 5 minor) has been identified whose research (75.1% NIH-funded) will greatly benefit from analyses performed with the requested instrumentation. The Trace Metals Lab (TML) in the Department of Environmental Health and Engineering at Johns Hopkins Bloomberg School of Public Health houses the only ICP-MS in the medical campus, which includes the School of Medicine (SOM) and the School of Public Health. The TML has been providing accurate, sensitive, and reliable trace metal analysis for health-related research since 2006. This shared resource provides funded investigators with technical expertise and access to large equipment for quantitative analysis of trace metals. The JHU TML is used heavily by NIH-funded grants supporting research that ranges from exposure to metals from e-cigarettes (R01), evaluation of Magnetic Hyperthermia Therapy (MHT) against infiltrating glioblastoma (R01) and liver cancer tumors (R01), assessment of arsenic exposure on immune responses to influenza (R01), Arsenic and other metal exposures in American Indian communities (R01), among others. The research that will benefit from this instrumentation will have enormous impacts on the disciplines of Medicine and Public Health. With ICP- QQQ, HPLC, microwave digestion, and Single Particle analysis capabilities, our lab will continue to advance the translation of basic biomedical research to treatments and cures, and will also contribute to prevention through strategies for reducing exposure to environmental pollutants for diverse populations. The number of basic and clinical investigators requiring analytical services of trace metals has experienced a rapid growth. As a result, usage of the existing ICP-MS instrument is currently at full capacity. The new instrumentation will also benefit studies that are not currently NIH funded, pilot projects to collect data for NIH grants, and student research projects. The ICP-MS capability of the JHU TML will also contribute to the long-range biomedical research goals of the Johns Hopkins University. This instrumentation will benefit research to understand basic cellular toxicity, risk factors for infection, develop important new therapeutic strategies against cancer, and advance our understanding of stroke mechanisms, heart disease, cancer survival, and immune responses. As outlined in this application, acquisition of this equipment will make this technology available to a large number of investigators, continuing with the goals of the Trace Metals Lab, the Department of Environmental Health and Engineering, the Bloomberg School or Public Health, the Johns Hopkins University, and the Biomedical community as a whole.