ICP-QQQ

项目概要/摘要： 该提案的总体目标是获得资金，以购买三重四极质谱仪和 新的微波热敏电阻，以取代14岁的安捷伦7500 ce ICP-MS和MARS 5微波。资金 将用于购买Agilent 8900 ICP-QQQ系统，该系统可与我们现有的Agilent 1260 HPLC系统将专门用于支持NIH资助的需要定量金属分析的研究者， 包括金属形态。已经确定了一组研究人员（6名主要研究人员，5名次要研究人员），他们的研究 (75.1% NIH资助）将大大受益于使用所要求的仪器进行的分析。微量 约翰霍普金斯彭博大学环境健康与工程系金属实验室（TML） 公共卫生学院拥有医学院唯一的ICP-MS，其中包括医学院 (SOM)和公共卫生学院TML一直提供准确、灵敏、可靠的痕量金属 自2006年以来，与健康相关的研究。这一共享资源为受资助的调查人员提供了 技术专长和获得大型设备进行微量金属定量分析。JHU TML是 大量用于NIH资助的资助赠款，从接触电子烟中的金属， (R01)，评价磁热疗（MHT）对浸润性胶质母细胞瘤（R 01）和肝脏的作用 癌症肿瘤（R 01）、砷暴露对流感免疫反应的评估（R 01）、砷和 美国印第安人社区的其他金属暴露（R 01）等。这项研究将有助于 这些仪器将对医学和公共卫生学科产生巨大的影响。关于ICP- QQQ、HPLC、微波消化和单颗粒分析功能，我们的实验室将继续推进 将基础生物医学研究转化为治疗方法，并通过以下方式促进预防： 减少不同人群接触环境污染物的战略。基本数量和 需要痕量金属分析服务的临床研究者经历了快速增长。因此，在本发明中， 现有ICP-MS仪器的使用目前已满负荷。新的仪器也将受益于 目前没有NIH资助的研究，为NIH赠款收集数据的试点项目，以及学生研究 项目JHU TML的ICP-MS能力也将有助于实现长期生物医学研究目标 约翰霍普金斯大学。这种仪器将有助于研究了解基本的细胞毒性， 感染的危险因素，开发重要的新的癌症治疗策略，并推进我们的 了解中风机制、心脏病、癌症存活率和免疫反应。正如本文所述， 应用，购置这一设备将使这一技术提供给大量的调查人员， 继续与微量金属实验室，环境健康与工程系， 布隆伯格学院或公共卫生，约翰霍普金斯大学，以及整个生物医学界。

Project Summary/Abstract: The overall goal of this proposal is to acquire funds to purchase a triple quadrupole mass spectrometer and a new microwave digestor to replace a 14-year old Agilent 7500ce ICP-MS and a MARS5 microwave. The funds will be used to purchase an Agilent 8900 ICP-QQQ system that can be coupled with our existing Agilent 1260 HPLC system which will be dedicated to support NIH-funded investigators requiring quantitative metal analysis, including metal speciation. A group of investigators (6 major, 5 minor) has been identified whose research (75.1% NIH-funded) will greatly benefit from analyses performed with the requested instrumentation. The Trace Metals Lab (TML) in the Department of Environmental Health and Engineering at Johns Hopkins Bloomberg School of Public Health houses the only ICP-MS in the medical campus, which includes the School of Medicine (SOM) and the School of Public Health. The TML has been providing accurate, sensitive, and reliable trace metal analysis for health-related research since 2006. This shared resource provides funded investigators with technical expertise and access to large equipment for quantitative analysis of trace metals. The JHU TML is used heavily by NIH-funded grants supporting research that ranges from exposure to metals from e-cigarettes (R01), evaluation of Magnetic Hyperthermia Therapy (MHT) against infiltrating glioblastoma (R01) and liver cancer tumors (R01), assessment of arsenic exposure on immune responses to influenza (R01), Arsenic and other metal exposures in American Indian communities (R01), among others. The research that will benefit from this instrumentation will have enormous impacts on the disciplines of Medicine and Public Health. With ICP- QQQ, HPLC, microwave digestion, and Single Particle analysis capabilities, our lab will continue to advance the translation of basic biomedical research to treatments and cures, and will also contribute to prevention through strategies for reducing exposure to environmental pollutants for diverse populations. The number of basic and clinical investigators requiring analytical services of trace metals has experienced a rapid growth. As a result, usage of the existing ICP-MS instrument is currently at full capacity. The new instrumentation will also benefit studies that are not currently NIH funded, pilot projects to collect data for NIH grants, and student research projects. The ICP-MS capability of the JHU TML will also contribute to the long-range biomedical research goals of the Johns Hopkins University. This instrumentation will benefit research to understand basic cellular toxicity, risk factors for infection, develop important new therapeutic strategies against cancer, and advance our understanding of stroke mechanisms, heart disease, cancer survival, and immune responses. As outlined in this application, acquisition of this equipment will make this technology available to a large number of investigators, continuing with the goals of the Trace Metals Lab, the Department of Environmental Health and Engineering, the Bloomberg School or Public Health, the Johns Hopkins University, and the Biomedical community as a whole.