OneMP Mass Photometer

OneMP 质量光度计

• 批准号: 10177547
• 负责人: Chad Brautigam
• 金额: $ 16.46万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177547
• 关键词: Adherence; Binding; Biophysics; Complement; Consumption; Data; Devices; Event; Funding; Glass; Image; Immobilization; Label; Light; Measures; Medical center; Methods; Mission; Modeling; Molecular; Photometry; Process; Research; Research Personnel; Resources; Sampling; Scientist; Speed; Structure; Technology; Texas; Thermodynamics; Time; Universities; Water; base; biophysical tools; experimental study; innovation; instrument; light scattering; macromolecule; molecular mass; programs; success

Project Summary In this proposal, a group of accomplished molecular scientists at the University of Texas Southwestern Medical Center requests funds for a mass photometry device. Specifically, the request is for the OneMP model of mass photometer manufactured by Refeyn Ltd. Mass photometry (MP) is a relatively new method that is very attractive to our user base. It uses the scattering of light as macromolecules adhere to at a water/glass interface (i.e. on a standard cover slip) to measure their respective molecular masses. It does this by recombining the scattered light with light reflected from the interface. The resulting images are processed to reveal the contrast in them as a function of time; the contrast can in turn be related directly to the molecular mass of the adherence event that gave rise to it. MP yields remarkably accurate molecular mass distributions, and it does so without labeling, immobilization, or interaction with a matrix. MP uses very small amounts of material, consuming only tens of picograms per experiment. It is also very fast, as an optimized experiment can be performed in 15 min or less. UTSW has a vigorous program of molecular research, and the MP fills a need for many of our scientists. Some will incorporate the MP into a workflow that will greatly amplify speed and efficiency with which they can produce structural data. For some of these researchers, the low sample consumption and accurate mass-distribution quantification of the method will open avenues of characterization heretofore unavailable to them. Others will use it to examine questions that can be probed only at very low sample concentration, for example thermodynamic characterization of very tight binding. Still others will utilize the technology as a fast and robust complement to other methods, enhancing scientific rigor. The OneMP will be overseen by the already existing Macromolecular Biophysics Resource, a successful core lab at UTSW with an excellent track record of incorporating and mastering new biophysical equipment. The commitment of UTSW to the success of the instrument is strong, and the overall benefit of its procurement is that it will strongly enhance the scientific, innovation, and educational missions of the University.

项目摘要 在这项提案中，德克萨斯大学西南医学分校的一群有成就的分子科学家 该中心要求为质量光度测量设备提供资金。具体地说，该请求是针对OneMP MASS模型的 质量光度法(MP)是Refeyn有限公司生产的光度计，是一种比较新的方法，非常有吸引力 到我们的用户群。它利用光的散射，因为大分子附着在水/玻璃界面上(即 标准盖片)来测量它们各自的分子质量。它通过重新组合分散的 具有从界面反射的光的光。对生成的图像进行处理，以显示其中的对比度 时间的函数；对比度可以直接与粘着事件的分子质量有关，该粘着事件 导致了它的出现。MP产生了非常准确的分子质量分布，而且它没有标记， 固定化，或与基质相互作用。MP使用的材料非常少，仅消耗数十 每个实验的皮卡数。它也非常快，因为优化的实验可以在15分钟或更短的时间内完成。 UTSW有一个强有力的分子研究计划，MP填补了我们许多科学家的需求。一些人 将MP合并到一个工作流程中，从而极大地提高他们生产的速度和效率 结构数据。对于其中一些研究人员来说，低样本消耗和准确的质量分布 该方法的量化将开辟迄今为止他们无法获得的表征途径。其他人会的 使用它来检查只有在很低的样本浓度下才能探测到的问题，例如 非常紧密结合的热力学表征。还有一些人将利用这项技术作为一种快速和强大的 与其他方法相辅相成，增强科学严谨性。OneMP将由现有的 大分子生物物理资源，UTSW成功的核心实验室，有着良好的记录 吸收和掌握新的生物物理设备。UTSW对成功的承诺 仪器强大，它的采购的总体好处是它将有力地提高科学性， 创新，和大学的教育使命。

项目成果

Ηigh-resolution structure of mammalian PI31-20S proteasome complex reveals mechanism of proteasome inhibition.

• DOI: 10.1016/j.jbc.2023.104862
• 发表时间: 2023-07
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Hsu, Hao-Chi;Wang, Jason;Kjellgren, Abbey;Li, Huilin;Demartino, George N.
• 通讯作者: Demartino, George N.

Structural insights into the assembly of the agrin/LRP4/MuSK signaling complex.

• DOI: 10.1073/pnas.2300453120
• 发表时间: 2023-06-06
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Xie T;Xu G;Liu Y;Quade B;Lin W;Bai XC
• 通讯作者: Bai XC