OneMP Mass Photometer

项目摘要 在这个提议中，德克萨斯西南医科大学的一组有成就的分子科学家 中心要求资金用于质量光度测定装置。具体而言，该请求是针对质量的OneMP模型 质量光度法（MP）是一种比较新的方法，非常有吸引力 我们的用户基础。它利用光的散射作为大分子粘附在水/玻璃界面（即， 标准盖玻片）以测量它们各自的分子量。它通过重组分散的 光与从界面反射的光。处理所得图像以揭示其中的对比度， 时间的函数;对比度又可以与粘附事件的分子量直接相关， MP产生了非常精确的分子量分布，并且它没有标记， 固定化或与基质相互作用。MP使用非常少量的材料，仅消耗数十 皮克/实验。它也非常快，因为优化的实验可以在15分钟或更短的时间内完成。 UTSW有一个强有力的分子研究计划，MP填补了我们许多科学家的需求。一些 将把MP纳入工作流程，这将大大提高他们生产的速度和效率 结构数据对于其中一些研究人员来说，低样本消耗和准确的质量分布 该方法的量化将开辟迄今为止他们无法获得的表征途径。其他人将 用它来检查只能在非常低的样品浓度下探测的问题，例如 非常紧密结合的热力学表征。还有一些人将利用该技术作为一种快速和强大的 与其他方法相辅相成，增强科学严谨性。OneMP将由现有的 高分子生物物理资源，一个成功的核心实验室在UTSW具有良好的跟踪记录， 整合和掌握新的生物物理设备。UTSW对成功的承诺 仪器是强大的，其采购的总体好处是，它将大大提高科学， 创新和大学的教育使命。

Project Summary In this proposal, a group of accomplished molecular scientists at the University of Texas Southwestern Medical Center requests funds for a mass photometry device. Specifically, the request is for the OneMP model of mass photometer manufactured by Refeyn Ltd. Mass photometry (MP) is a relatively new method that is very attractive to our user base. It uses the scattering of light as macromolecules adhere to at a water/glass interface (i.e. on a standard cover slip) to measure their respective molecular masses. It does this by recombining the scattered light with light reflected from the interface. The resulting images are processed to reveal the contrast in them as a function of time; the contrast can in turn be related directly to the molecular mass of the adherence event that gave rise to it. MP yields remarkably accurate molecular mass distributions, and it does so without labeling, immobilization, or interaction with a matrix. MP uses very small amounts of material, consuming only tens of picograms per experiment. It is also very fast, as an optimized experiment can be performed in 15 min or less. UTSW has a vigorous program of molecular research, and the MP fills a need for many of our scientists. Some will incorporate the MP into a workflow that will greatly amplify speed and efficiency with which they can produce structural data. For some of these researchers, the low sample consumption and accurate mass-distribution quantification of the method will open avenues of characterization heretofore unavailable to them. Others will use it to examine questions that can be probed only at very low sample concentration, for example thermodynamic characterization of very tight binding. Still others will utilize the technology as a fast and robust complement to other methods, enhancing scientific rigor. The OneMP will be overseen by the already existing Macromolecular Biophysics Resource, a successful core lab at UTSW with an excellent track record of incorporating and mastering new biophysical equipment. The commitment of UTSW to the success of the instrument is strong, and the overall benefit of its procurement is that it will strongly enhance the scientific, innovation, and educational missions of the University.