An innovative instrument cluster for the integrative behavioral analysis of mouse mutants with perturbed neuronal connectivity

用于对神经元连接受到干扰的小鼠突变体进行综合行为分析的创新仪器组

• 批准号: 10176986
• 负责人: Bogdan Beirowski
• 金额: $ 17.32万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-15 至 2022-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176986
• 关键词: Animals; Applications Grants; Automation; Behavior; Behavioral; Biological Markers; Characteristics; Custom; Disease Progression; Disease model; Distress; Equilibrium; Equipment; Funding; Gait; Gene Mutation; Grant; Impairment; Information Technology; Ink; Measurement; Methods; Modeling; Monitor; Morphologic artifacts; Mus; Mutant Strains Mice; Nerve Degeneration; Neuroglia; Neurons; Pattern; Phenotype; Reproducibility; Research Personnel; Sensory; Statistical Data Interpretation; System; Systems Analysis; axonal degeneration; base; computerized; data acquisition; gait examination; innovation; instrument; myelination; neurobehavioral; spatiotemporal; tool; treatment group

Project Summary/Abstract: This S10 SIFAR grant application seeks funds to acquire an integrated instrument cluster to allow the sensitive, reliable, and unbiased neurobehavioral phenotyping of a range of mouse mutants from five major users modeling neurodegenerative conditions. These conditions arise from gene mutations in glial cells resulting in aberrant myelination and axon degeneration. The synergistic instrument cluster from Noldus Information Technology comprises the Noldus CatWalk XT, the Noldus ErasmusLadder, and the Noldus EthoVision XT open-field analysis system with custom configuration for simultaneous observation of up to eight animals. The CatWalk XT is a highly sensitive video-based automated gait analysis tool quantifying spatiotemporal characteristics of gait patterns along with paw print intensities and ambulatory velocities of mice. The ErasmusLadder is an automated system for the assessment of balance, coordination and locomotor capacity in mice. The EthoVision XT open-field video-tracking system allows automated monitoring and statistical analysis of activity, numerous ambulatory parameters, and stereotypic behavior in freely moving mice in batch-acquisition mode. The five investigators currently use conventional neurobehavioral methods (e.g., ink-based footprint, rotarod, pole-climb analysis) that are particularly laborious, susceptible to artefacts, prone to high variability, inherently subjective, and distressing to animals. The complementary instrument cluster will minimize these shortcomings, while in parallel increasing throughput via the automation and computerization of data acquisition. The equipment will also synergize with another instrument cluster accessible to the investigators for the specific analysis of sensory impairment. All instruments together will allow the investigators to comprehensively study sensorimotor alterations in their neurodegenerative mouse disease models. This will allow for the identification of more reliable neurobehavioral biomarkers, which will facilitate the measurement of disease progression and comparision of treatment groups. The base grant projects that support this proposal will greatly benefit from the additional capabilities of the tripartite instrument cluster as well as from enhanced rigor, reproducibility, and transparency.

项目摘要/摘要： 此S10 SIFAR赠款申请寻求资金，以收购一个集成的仪表组，以 允许对一系列小鼠进行敏感、可靠和无偏见的神经行为表型分析 来自五个主要使用者的突变体模拟神经退行性疾病。这些条件 源于胶质细胞的基因突变，导致异常的髓鞘形成和轴突 退化。来自NOLDUS信息技术的协同式仪表组 包括NOLDUS T型台XT、NOLDUS Erasmus梯子和NOLDUS ethoVision 用于同时观测UP的定制配置的XT开场分析系统 给了八只动物。T型台XT是一种高度敏感的基于视频的自动步态分析 步态模式时空特征与爪印强度的量化工具 以及小鼠的活动速度。ErasmusLader是一种自动系统，用于 评估小鼠的平衡、协调和运动能力。EthoVision XT 开场视频跟踪系统允许自动监控和统计分析 自由活动的小鼠的活动性、众多的步行参数和刻板行为 批量采集模式。这五名调查人员目前使用的是传统的神经行为 特别费力的方法(例如，基于墨迹的足迹、旋转杆、杆子爬升分析)， 容易受到人工制品的影响，易于高度多变性，固有的主观性，并令人痛苦地 动物。互补性组合仪表将最大限度地减少这些缺点，而在 通过数据采集的自动化和计算机化并行增加吞吐量。 该设备还将与可访问的另一个组合仪表协同工作 调查人员对感觉障碍的具体分析。所有乐器加在一起将 使研究人员能够全面研究患者的感觉运动改变 神经退行性疾病小鼠模型。这将允许识别更多 可靠的神经行为生物标志物，这将有助于疾病的测量 治疗组的进展和比较。支持这一点的基本赠款项目 提案将大大受益于三方组合仪表组的额外能力 以及增强的严密性、再现性和透明度。