SkyScan 1276: Multiscale Micro-CT SystemLaboratory

SkyScan 1276：多尺度微型 CT 系统实验室

• 批准号: 10177395
• 负责人: Sean P Marrelli
• 金额: $ 36.76万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177395
• 关键词: 3-Dimensional; Address; Anesthesia procedures; Animals; Biological; Breathing; Caliber; Charge; Computer software; Computers; Contract Services; Data Storage and Retrieval; Detection; Dimensions; Electrocardiogram; Faculty; Fees; Funding; Heart Rate; Image; Institution; Isoflurane; Measurement; Medical center; Monitor; Movement; Mus; Neurology; Physiologic Monitoring; Radiation Dosage; Radiation Dose Unit; Radiation exposure; Rattus; Research Personnel; Research Support; Resolution; Resources; Respiration; Rodent; Roentgen Rays; Scanning; Secure; Shapes; Specimen; Speed; System; Temperature; Texas; United States National Institutes of Health; Wages; base; cost; equipment acquisition; foot; high resolution imaging; image reconstruction; imaging capabilities; improved; in vivo; in vivo imaging; instrument; medical schools; meter; microCT; operation; programs; sensor

ABSTRACT We are requesting funds to purchase a Bruker SkyScan 1276 High-Resolution In Vivo X-Ray Microtomograph (micro-CT scanner). This instrument will directly support the research programs of 18 investigators (Users), including 11 NIH-funded Users (with 16 different projects). The S10 Users represent faculty from 6 different departments within the McGovern Medical School at UTHealth (MMS) and one from a neighboring institution. The four Major Users represent three different departments within the MMS. The requested instrument would be the first of its kind in the MMS, and thus a valuable new resource for performing high resolution in vivo imaging of rats and mice. This state-of-the-art micro-CT scanner offers the ability to perform in vivo or ex vivo measurements at resolution up to 2.8 um. The dimensions of the scanner can accommodate specimens up to 160 mm (bore diameter) with a scanning field of view of 80 mm. The majority of the applications will include mice and rats, capitalizing on the instrument’s high image resolution – essential for many of the applications with these smaller animals. Another key application of this instrument will be longitudinal in vivo imaging. Features unique to the SkyScan 1276 instrument allow for significant reduction of radiation exposure, thus permitting repeated imaging with minimal biological effect from X-ray exposure. The instrument is equipped with an anesthesia system (isoflurane) and integrated physiological monitoring (including a breathing sensor, ECG, temperature stabilization, and body movement detection). The monitoring system allows for gated acquisition based on respiration or heart rate for improved in vivo resolution. Peak X-ray energy is adjustable from 20 to 100 kV and a proprietary spatial beam shaper reduces radiation dose up to 5 fold. An integrated meter keeps track of animal radiation dosage. The instrument is supplied with a powerful computer for acquisition and processing, capable of high-speed image reconstruction (NRecon 3D) and with ample data storage. Offline analysis is available on any computer through free viewer and analysis software from Bruker. The footprint of the instrument is small, requiring only 6 feet of a standard bench. The Department of Neurology has designated a space for the instrument (room MSB 3.508), which is secure and convenient to MMS users. The Institution has committed to support 88% of the service contract cost (to 5 years) and salary of a 50% dedicated technician to operate and maintain the SkyScan 1276. The instrument will be operated on a very low fee-for-use basis ($15-45/hr) to recover operation costs. Usage for NIH-funded projects will be >75%, with at least 10% of peak usage remaining available for non-S10 Users. To support junior investigators or un-funded investigators, up to 10% usage will be provided free of charge. In summary, we are requesting funds to purchase an instrument which will address the absence of high resolution in vivo micro-CT imaging capabilities for rodents at the MMS. The instrument will serve as a state-of-the-art resource to the S10 Users, investigators throughout the MMS, and even neighboring institutions on the Texas Medical Center campus.

抽象的 我们要求资金购买Bruker Skyscan 1276体内X射线仪的高分辨率 （Micro-CT扫描仪）。该工具将直接支持18位研究人员（用户）的研究计划 包括11个由NIH资助的用户（有16个不同的项目）。 S10用户代表来自6个不同的教师 Uthealth（MMS）的McGovern医学院内的部门，来自邻近机构的部门。 四个主要用户代表MMS中的三个不同部门。请求的仪器将是 MMS中的第一个，因此是在体内成像中执行高分辨率的宝贵新资源 老鼠和老鼠。这款最先进的微型CT扫描仪具有在体内或Ex Vivo进行的能力 分辨率高达2.8 um时的测量。扫描仪的尺寸可以容纳标本 160毫米（直径），扫描视图为80毫米。大多数应用程序将包括小鼠 和老鼠，利用该乐器的高图像分辨率 - 对于许多应用程序至关重要 较小的动物。该仪器的另一个关键应用将是体内成像中的纵向。功能独特 到Skyscan 1276仪器可以大大减少辐射暴露，从而可以重复 X射线暴露的成像具有最小的生物学作用。该仪器配备了麻醉 系统（异氟烷）和集成的物理监测（包括呼吸传感器，ECG，温度 稳定和身体运动检测）。监视系统允许基于 改善体内分辨率的呼吸或心率。峰X射线能量可从20到100 kV调节，并且 专有的空间束塑形器可将辐射剂量降低到5倍。集成的仪表跟踪动物 辐射剂量。该仪器提供了一台功能强大的计算机，用于获取和处理，能够 高速图像重建（NRECON 3D）和充足的数据存储。离线分析可用于 通过Bruker的免费观看器和分析软件的任何计算机。乐器的足迹很小， 仅需要6英尺的标准长凳。神经病学系指定了一个空间 仪器（MSB 3.508室），对MMS用户来说是安全且方便的。该机构已致力于 支持服务合同成本的88％（至5年）和50％专用技术人员的薪水，并 维护Skyscan 1276。该仪器将以非常低的费用基础（$ 15-45/hr）进行操作 收回运营成本。 NIH资助的项目的使用量将> 75％，至少剩余的10％ 可用于非S10用户。为了支持初级调查人员或未资助的调查人员，最多使用10％ 免费提供。总而言之，我们要求资金购买一种将解决的工具 在MMS处啮齿动物的体内微分离学成像能力缺乏高分辨率。乐器将 作为S10用户，整个MMS甚至附近的调查人员的最先进资源 德克萨斯州医疗中心校园的机构。